EMAGE mouse gene expression spatial database

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EMAGE:31123

Neurod6 neurogenic differentiation 6 ( MGI:106593)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005190_01	euxassay_005190_02	euxassay_005190_03	euxassay_005190_04	euxassay_005190_05

euxassay_005190_06	euxassay_005190_07	euxassay_005190_08	euxassay_005190_09	euxassay_005190_10

euxassay_005190_11	euxassay_005190_12	euxassay_005190_13	euxassay_005190_14	euxassay_005190_15

euxassay_005190_16	euxassay_005190_17	euxassay_005190_18	euxassay_005190_19	euxassay_005190_20

euxassay_005190_21	euxassay_005190_22	euxassay_005190_23	euxassay_005190_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

not examined

regional

not examined expression: see section 17

spinal cord mantle layer

moderate

regional

moderate expression: see section 12 weak expression: see section 10 11 13 14 15

inner ear

weak

regional

weak expression: see section 06 07 17 18 20

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 10 11 12 17

telencephalon mantle layer

moderate

regional

moderate expression: see section 05 06 07 17 18 19 20 weak expression: see section 01 02 03 04 08 09 10 11 12 13 14 15 21 22 23 24 not examined expression: see section 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8840
Entity Detected:	Neurod6, neurogenic differentiation 6 ( MGI:106593)
Sequence:	sense strand is shown >T8840 CTAGAGGCTCCAGGAGACGATGCGACACTCAGCCTGAAAAGATTTGGAAGATCCAAAATGAAAACTGATT ATTGAATGAAATTAAAACCTAAGGTAAATTAAGGTTAAAGAACCATGTTAACACTACCGTTTGACGAGTC TGTCGTAATGCCCGAATCCCAGATGTGCAGAAAGTTTGCTAGACAATGTGAGGACCAGAAACAAATTAAG AAACCAGAGAGCTTTCCAAAACAAGTTGTCCTTCGAGGAAAGAGCATTAAAAGGGCCCCTGGAGAAGAAA CCGAGAAAGAAGAGGAGGAAGAAGACAGAGAGGAAGAAGATGAGAATGGCTTGTCCAGAAGGAGGGGGCT CAGGAAAAAAAAGACCACCAAACTACGACTGGAAAGGGTCAAGTTCAGGAGACAGGAAGCTAATGCGCGC GAGAGGAACCGGATGCACGGCCTCAATGATGCTCTGGACAATTTGCGAAAAGTGGTCCCCTGTTACTCTA AAACCCAAAAACTGTCCAAAATAGAAACTTTACGACTGGCCAAAAATTACATCTGGGCACTTTCTGAAAT TCTGAGGATTGGCAAGAGACCGGATCTGCTCACGTTCGTCCAAAACTTATGCAAAGGTCTTTCCCAGCCA ACTACAAACTTGGTGGCAGGCTGCTTACAGCTCAACGCCAGAAGTTTCCTGATGGGTCAGGGTGGGGAGG CTGCCCACCACACAAGGTCACCCTACTCCACATTCTACCCACCCTACCACAGCCCTGAGCTGGCCACTCC CCCAGGGCATGGGACTCTTGATAATTCCAAGTCCATGAAACCCTACAATTACTGCAGTGCATATGAATCC TTCTATGAAAGTACCTCCCCTGAGTGTGCCAGCCCTCAGTTTGAAGGTCCCTTAAGTCCTCCCCCAATTA ACTATAATGGGATATTTTCCCTGAAGCAAGAAGAAACCTTGGACTATGGCAAAAATTACAATTATGGCAT GCATTACTGTGCAGTGCCACCCAGGGGTCCCCTTGGGCAGGGTGCCATGTTCAGGTTGCCCACCGACAGC CACTTCCCTTACGACTTACATCTGCGCAGCCAATCTCTCACTATGCAAGATGAATTAAATGCAGTTTTTC ATAATTAATGAGGAAAATGAAAATAAACAGTGGTCATTCACCTCCCACTCTAATTAAGGCAAAGCAGATG CTTGTGGGCTGCGTAATTGGCACAACTCTATCTAAGGTGTTTACTAGTTTCTGAAGTGTGTCTCAAAGAT TGTGACCATTTTCTATGTCATAATAAATCCCTTTTCGTATGAGAACTTCCTTTCCTTCCCTCTTGTCTGT ATCACACTGTGATTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTACTGGCAGAATATTT CTTTCTTGTTTTAGTTTCTTTCAAATTCACTTAATTTGTTTGAACAAGGTGTCTAAGATGTTGCTGAATA AAGACATGCACACAGCATACTTCAATGTCTATTTCAGTTGTACAGTTATGATGAAAATGCATGTTATAAA AATCAGATGAGTAAAATGTGTTTATAATTACTAGGATTCATATATGTATCTCTGAAATTTTAGTTTTTAA AATATTAAGAGCTAACCATGAAATTAAAAGGTGCATTTGGGGATGCACAACGGTATCAAAAGCTATGCAA TTTTCTGTTTATTAGGGACAAAAATAAGTGTATTCAGTTGGTAACAACTATTCCTCTTCAAGCATTTTCA GAGGAGGAAACACGGTATTTGGGGGAGGTTATCAGTGTCATAATTTGGGGACAATTAATTCAATCATGAA GAAAAAAAATATTAGCACTTGTTTTGTATTGTTCAGGATTTTTCTGTACAGGTTTGTTACAGTGTATAAT TGTGTTTTCCATCCTACAGTTTAAAAGCAATTAAACATAGATATTTCCACTTAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6411826 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6411826 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005190 same experiment
	EMAGE:29196 same embryo
	EMAGE:29244 same embryo
	EMAGE:29225 same embryo
	EMAGE:29246 same embryo
	EMAGE:29227 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source