EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31140

Psapl1 prosaposin-like 1 ( MGI:1924193)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002938_01	euxassay_002938_02	euxassay_002938_03	euxassay_002938_04	euxassay_002938_05

euxassay_002938_06	euxassay_002938_07	euxassay_002938_08	euxassay_002938_09	euxassay_002938_10

euxassay_002938_11	euxassay_002938_12	euxassay_002938_13	euxassay_002938_14	euxassay_002938_15

euxassay_002938_16	euxassay_002938_17	euxassay_002938_18	euxassay_002938_19	euxassay_002938_20

euxassay_002938_21	euxassay_002938_22	euxassay_002938_23	euxassay_002938_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

strong

regional

strong expression: see section 07 08 24 weak expression: see section 09 10 23

naris

strong

regional

strong expression: see section 14 15 16 18 19 20

rectum

strong

regional

strong expression: see section 16 17

urethra of male

strong

regional

strong expression: see section 15 16 17

bladder

strong

regional

strong expression: see section 15 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1043
Entity Detected:	Psapl1, prosaposin-like 1 ( MGI:1924193)
Sequence:	sense strand is shown >T1043 TCCTCNAGNCTGTTGGCCTACTGNGAGTTTTATAGCGAGAGCCAAGCAGAGGGAGCAGCCAGTGGAACCA TGGGCACTATGCTGTGCGCTCTGATTCTCTGGTCTGGCCTCCTGGGGGCTGCCAGGGCCAGCCCCATCTC AGTTCCCCGTGAGTGTGCCAAGGGTTCGGAGGTATGGTGTCAGGACCTGCAGGCAGCTGCAAAGTGTCGG GCCGTGAGGCACTGCCAGAGTGCTGTCTGGAACAAGCCCACAGTCAAGTCCCTGCCCTGCAGTGTGTGCC AGGATGTGGCCGCGGCTGCTGGCAATGGGGTGAACCCAGGTGCTACAGAGTCGGATATTTTGACATCAGT AATGAAGACCTGTGAGTGGCTTCCAAGCCAGGAGTCTTCAGCTAAGTGCAAGTGGATGGTGAACAACCAC AGCGCAGCTGTCCTGAGCATGCTCAGTGGCGCTCAGGAGACTGACCTGGCTTCTGTGTGCACTGCGCTCA CCCTCTGTGAACCATTACAGAGGCACCTAGCTGAGACTACCTCGGAGAGACCGCTGACCCAGGAGGATGC AAATGAGGTGA
Notes:	The probe template was PCR amplified from IMAGE:2088199 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2088199 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002938 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source