EMAGE mouse gene expression spatial database

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EMAGE:31200

Rasal1 ( MGI:1330842)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002215_01	euxassay_002215_02	euxassay_002215_03	euxassay_002215_04	euxassay_002215_05

euxassay_002215_06	euxassay_002215_07	euxassay_002215_08	euxassay_002215_09	euxassay_002215_10

euxassay_002215_11	euxassay_002215_12	euxassay_002215_13	euxassay_002215_14	euxassay_002215_15

euxassay_002215_16	euxassay_002215_17	euxassay_002215_18	euxassay_002215_19	euxassay_002215_20

euxassay_002215_21	euxassay_002215_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6018
Entity Detected:	Rasal1, ( MGI:1330842)
Sequence:	sense strand is shown >T6018 CCCACGCGTCCGCCCACGCGTCCGGAAGAGAAGATGCAAGGGGATGTTCGAGGTCCCTCCCTCCACTCGC ATCAGACCCCCAGGGAGCGTGCGCCGCCTGCCGCCCAGAGAGACCCTGCATCCAGCCCACTCGCACCCCA GACCCGGACACGTGGGAACCGGCTGACAGCGTGCTTGGCCCGCAGCTGATGACATGTAGGGTCACCTCCT GGTCCAGCCTCAGAACCTCGGCAGACTCCTTGCCTGGAAAGTTTGGGACTGTGCGACATGGCCAAGAGCG GCTCGCTGAGTATCCGCGTGGTGGAGGGACGAGCACTGCCCGCCAAGGACGTGTCTGGAAGCAGTGACCC CTATTGTCTGGTGAAAGTGGATGACCAAGTGGTGGCCAGGACAGCAACCATCTGGAGGAGCCTGAGCCCC TTTTGGGGGGAGGAGTACACCGTTCACCTTCCATTGGACTTCCACCACCTGGCCTTCTACGTGCTGGATG AGGACACCGTTGGACACGATGACATCATTGGGAAGATCTCATTGAGCAAAGAGGCGATCACAGCCGACCC TCGAGGGATCGACAGCTGGATCAACCTGAGCCGAGTGGATCCAGACGCTGAAGTACAGGGTGAGGTCTGC CTGGATGTGAAGCTATTGGAGGATGCTCGGGGCCGCTGCCTCCGCTGCCACGTGAGACAGGCCAGGGACC TGGCCCCCCGGGACATCTCTGGCACATCGGACCCATTTGCCCGTGTGTTCTGGGGCAACCATAGTTTGGA AACTTCGACCATCAAGAAGACCCGCTTTCCACACTGGGATGAGGTGTTGGAGCTGCGGGAGGCTCCGGGG ACCACGTCCCCGCTGCGAGTGGAACTCTGGGATTGGGACATGGTGGGCAAGAATGACTTCCTGGGTATGG TGGAGTTCACCCCACAGACCCTGCAGCAGAAGCCACCTAATGGCTGGTTCCGGCTCCTGCCCTTTCCTAG AGCTGAGGATTCTGGGGGGAGCCTGGGTGCCCTGCGGCTGAAGGTGCGCCTCACTGAGGACCGGGTCCTG CCTTCCCAGTACTACCAGCCTCTCATGGAACTGCTTCTGGAGTCGGTGCAAGGGCCGGCTGAGGAGGACA CCACCAGCCCCCTGGCTCTGCTAGAGGAGCTGGCCTCTGGGGACTGTCGTCAGGACCTTGCCACCAAGCT GGTGAAGCTGTTCCTGGGCCGGGGCCTGGCCGGGCCCTTTCTAGATTATCTCACAAGGCGTGAGGTGGCT CGAACCAATGACCCCAACACCCTCTTCCGTTCTAACTCCTTGGCATCCAAGTCGATGGAACAGTTCATGA AGCTCGTGGGCATGCGCTACCTGCACGAGGTCCTGAGGCCAGTGATCAGCCGCGTCTTCGAGGAGAAGAA ATATATGGAACTGGACCCTTGCAAGATGGACCTGAACCGCTCTCGGAGGATCTCCTTCAAGGGCACGCCC ACAGAGGAGCAGGTACGGGAGACCAGCCTGGGGCTGCTGACCGGATACCTAGGGTCCGTTGTGGACGCCA TCGTGAGCTCTACAGGGCGCTGCCCACTTGCCTTGCGCTTGGCCTTTAAGCAGCTCCAGCGGTGTGTGGA GAAGCGCTTCTCTGGGATAGAGCATCAGGATGTGAAGTACCTGGCCATCAGTGGCTTCCTCTTTCTGCGG TTCTTTGCGCCTGCCATCCTCACACCGAAACTGTTTGACCTCAGAGACCAGCACGCAGACCCCCAGACCA GCCGTTCCCTGCTGCTGCTCGCCAAGGCTGTGCAGAGCATTGGGAACCTGGGCCAGCAGCTGGGCCAGGG CAAGGAGCAGTGGCTAGCCCCGCTCCATCCCTTCCTGCTGCAGAGCATCTCACGTGTGAGGGACTTCCTG GACCAGCTGGTGGATGTGGACGAGGATGAGGAGGCCGGGGGTCCAGCCTGCGCCCTGGTCCAACCCTCAA CCATTGTTCGAGAAGGCTTCCTGCTGAAGCGCAAGGAGGAGCCTGGAGGCCTGGCCACGCGCTTTGCCTT CAAGAAGCGCTACTTCCGGCTGAGTGGGCGGGACCTCTCCTACTCCAAGACTCCGGAGTGGCAGGTTCAC ACATCCATCCCGCTGTCCTGCATCCGGGCTGTGGAGCATGTGGACGAGGGTGCCTTTCAACTGCCACACG TCATGCAGGTGGTGACACAGGATGGCGCCGGGACGTCACACACCACCTACCTCCAGTGTAAGAACGTGAA TGACCTCAACCAATGGCTGTCAGCCCTGCGCAAAGCCAGTGCCCCCAACCCGGGCAAGCTGGTTGCCTGC CACCCTGGTGCCTTCCGCAGCGGGCGCTGGACCTGCTGCCTCCAGGCTGAGCGCTCAGCTGCTGGTTGTA GCCGCACACACTCAGCCATCACACTGGGAGACTGGAGTGACCCACTGGATCCTGACGCCGAGGCCCAGGC AGTGTATCGCCAGCTGCTCCTGGGGCGGGACCAGCTCAGGCTGAAACTGCTGGAGGATTCCAGCCTGGAC ACAGAAGTGGACCCTGGGAGGGACTCCAGTGCCACAGACGGGCCCTGTGCTGAGGTTCTGGCCCAGCAGA GAGCAGCCACAACCCACCTGCTGCAGGTACTTGAAGACTTGGAGCAAGCCCACGAGGAGTTCCAGAAACG GGGATAGGAGCAAGTGGCCCTCTAGGATGCTGGATGCTGAGAAAGTGCCAGAGCCAGCTGGGGACAGAGC CATGGGGCCTCCCTGAGCATTCTTTGGCCCAGGCATCTTCTTGAGGCCCCCTTCTTCACTGCTTGGGGCC TCCCAGACAAAGCCTGGTCCTGTGTGCTCTGTAGCCAGCCAGGGCATAGGAGCCTTTGGTAGGGCCCAGT CTTGTACTGACCAAACCTCACTTTCTTCTCTGAGCCTGGAGTCTCAGCTTCACTGGACACAGCCCTTCCA ATACCCGTGGCGCCCATCTCACCCCAAGCTGCTGGATACAGCTGTACCTGAACGCCAACCCTGGCGGACT CAGAAAAGCATTCAATAGGCTTGGCCAGGAACTTCCTGTTTTCCACTTCTGCTAGCCACTGTGACCTCAC AGATCCTTCTGTATTCCACTCTCATCTGGCAGCCCCAACCAGGTGTTCTCAGAAGAACGGACTGGGGGCC CAGCATGCCAATTCAGAGCCTCGAAGCCGGACACAGGCTCTTCCTCTCTCTTATACCCTGTATAAAGTGA TGCGTGAGCATGAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3488024 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3488024 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002215 same experiment
	EMAGE:31183 same embryo
	EMAGE:30215 same embryo
	EMAGE:30236 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source