EMAGE mouse gene expression spatial database

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EMAGE:31201

Tubb3 tubulin, beta 3 class III ( MGI:107813)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002698_01	euxassay_002698_02	euxassay_002698_03	euxassay_002698_04	euxassay_002698_05

euxassay_002698_06	euxassay_002698_07	euxassay_002698_08	euxassay_002698_09	euxassay_002698_10

euxassay_002698_11	euxassay_002698_12	euxassay_002698_13	euxassay_002698_14	euxassay_002698_15

euxassay_002698_16	euxassay_002698_17	euxassay_002698_18	euxassay_002698_19	euxassay_002698_20

euxassay_002698_21	euxassay_002698_22	euxassay_002698_23	euxassay_002698_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

strong

regional

strong expression: see section 09 10 11 12 13 19 20 21

not examined

regional

not examined expression: see section 01 02 03 04 24

neural retina

moderate

regional

moderate expression: see section 01 02 03 04 05 24

trigeminal v ganglion

strong

regional

strong expression: see section 05 06 07 08 09 19 20 21 22 23 24

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13 15 16 17 18 19

cervical ganglion

moderate

regional

moderate expression: see section 10 11 19

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 08 09 10 19 20 21 22

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 18

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 09 10 20 21

thoracic ganglion

moderate

regional

moderate expression: see section 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5303
Entity Detected:	Tubb3, tubulin, beta 3 class III ( MGI:107813)
Sequence:	sense strand is shown >T5303 GCTGTCCGCCTGCCTTTTCGTCTCTAGCCGCGTGAAGTCAGCATGAGGGAGATCGTGCACATCCAGGCCG GCCAGTGCGGCAACCAGATAGGGGCCAAGTTCTGGGAGGTCATCAGCGATGAGCACGGCATAGACCCCAG CGGCAACTATGTAGGGGACTCAGACCTGCAGCTGGAGCGCATCAGCGTATACTACAATGAGGCCTCCTCT CACAAGTATGTGCCCAGGGCCATTCTGGTGGACTTGGAACCTGGAACCATGGACAGTGTTCGGTCTGGCG CCTTTGGACACCTATTCAGGCCCGACAACTTTATCTTTGGTCAGAGTGGTGCTGGCAACAACTGGGCCAA AGGGCACTATACGGAGGGCGCGGAGCTGGTGGACTCAGTCCTAGATGTCGTGCGGAAAGAGTGTGAGAAT TGTGACTGCCTGCAGGGCTTCCAGCTGACACACTCACTGGGTGGGGGCACAGGCTCAGGCATGGGCACAC TGCTCATCAGCAAGGTGCGTGAGGAGTACCCCGACCGCATCATGAACACCTTCAGCGTGGTGCCTTCACC CAAAGTGTCGGACACTGTGGTGGAGCCCTACAACGCCACCCTGTCCATCCACCAGCTAGTGGAGAACACA GACGAGACCTACTGCATCGACAATGAAGCCCTCTACGACATCTGCTTCCGCACCCTCAAGCTGGCCACAC CCACCTATGGGGACCTCAACCACCTTGTGTCTGCCACCATGAGTGGAGTCACCACCTCCCTTCGATTCCC TGGTCAGCTCAATGCCGACCTCCGCAAGCTGGCTGTGAACATGGTGCCGTTCCCACGTCTCCACTTCTTC ATGCCCGGCTTCGCCCCACTTACAGCCCGGGGCAGCCAGCAGTACCGTGCCCTGACGGTGCCTGAGCTCA CGCAGCAGATGTTCGATGCCAAGAACATGATGGCTGCCTGTGACCCGCGCCACGGTCGCTACCTGACCGT GGCCACTGTCTTCCGTGGGCGCATGTCTATGAAGGAGGTGGACGAGCAGATGCTGGCCATCCAGAGTAAG AACAGCAGCTACTTCGTGGAGTGGATCCCCAACAACGTCAAGGTAGCCGTGTGTGACATCCCACCCCGTG GGCTCAAAATGTCATCCACCTTCATTGGCAACAGCACGGCCATCCAGGAGCTGTTCAAACGCATCTCGGA GCAGTTCACAGCCATGTTCCGGCGCAAGGCCTTCCTGCACTGGTACACGGGCGAGGGCATGGATGAGATG GAGTTCACCGAGGCCGAGAGCAACATGAATGACCTGGTGTCCGAGTACCAGCAGTACCAGGACGCCACTG CGGAGGAGGAGGGGGAGATGTATGAAGATGATGACGAGGAATCGGAAGCCCAGGGGCCCAAGTGAAGTTG CTCGCAGCTGGGGTGTGGGGCCAAGTGGCAGCCAGGGCCAAGACAAGCAGCATCTGTCCCCCCCAGAGCC ATCTAGCTACTGACACTGCCCCCAGCTTTGCTTCTCACCAGCTCATTAGGGCTCCCAGGTTAAAGTCCTT CAGTATTTATGGCCACCCCCACTCCATGTGAGTCCACTTGGCTCTGTCCTCCCCATTTTAGCCACCTCTG TATTTATGTTGCTTATTCGTCTGTTTTTATGGTTTGTTTTGTTTTTTTACTGGGTTGTGTTTATATTCGG GGGGAGGGGTATACTTAATAAAGTTACTGCTGTCTGTCAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5363050 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5363050 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002698 same experiment
	EMAGE:30209 same embryo
	EMAGE:30230 same embryo
	EMAGE:30252 same embryo
	EMAGE:30248 same embryo
	EMAGE:30247 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source