EMAGE mouse gene expression spatial database

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EMAGE:31220

Hsd3b4 ( MGI:96236)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000488_01	euxassay_000488_02	euxassay_000488_03	euxassay_000488_04	euxassay_000488_05

euxassay_000488_06	euxassay_000488_07	euxassay_000488_08	euxassay_000488_09	euxassay_000488_10

euxassay_000488_11	euxassay_000488_12	euxassay_000488_13	euxassay_000488_14	euxassay_000488_15

euxassay_000488_16	euxassay_000488_17	euxassay_000488_18	euxassay_000488_19	euxassay_000488_20

euxassay_000488_21	euxassay_000488_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 17 18 19 moderate expression: see section 01 02 10 11 12 13 14 15 16 20 weak expression: see section 03 04 05 06 07 08 09 22

midbrain ventricular layer

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 21

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 10 11 weak expression: see section 05 06

metencephalon rest of alar plate ventricular layer

strong

homogeneous

strong expression: see section 11 18 19 20 moderate expression: see section 01 02 03 10 12 13 14 weak expression: see section 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1452
Entity Detected:	Hsd3b4, ( MGI:96236)
Sequence:	sense strand is shown >T1452 CCTCGAGNCTGTTGGCCTACTGGTCTTTCTCTTTCCTCAGCCTGTGCATCACTTCCTCTGAGTACCTTAT ATCACATCTGCACCCTCAACTATCTGATTCCTGTGTTGACCATGCCTGGGTGGAGCTGCCTGGTGACAGG AGCAGGAGGGTTTCTTGGCCAGAGGATTGTCCGAATGTTGGTGCAGGAGGAAGAGCTGCAGGAGATCAGA GCCCTGTTCAGGACCTTCGGTCGAAAACAGGAAGAGGAATTGTCCAAGCTGCAGACAAAGACCAAGGTGA CAGTACTGAAGGGAGACATCCTGGATGCCCAGTGCCTGAAGAGAGCCTGCCAGGGCATGTCTGCTGTCAT CCACACCGCTGCTGCTATTGACCCCCTTGGTGCCGCTTCCAGACAGACCATCCTAGATGTCAATCTGAAA GGTACTCAGCTCCTACTGGATGCTTGTGTGGAAGCCAACGTGCCAACATTCATCTACAGCAGCTCAGTGC TTGTGGCTGGACCAAATTCCTACAAGGAGATCATCCTGAATGCCCATGAGGAGGAGCATCATGAAAGCAC ATGGTCTAACCCATACCCATACAG
Notes:	The probe template was PCR amplified from IMAGE:2520050 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2520050 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000488 same experiment
	EMAGE:31218 same assay
	EMAGE:31219 same assay
	EMAGE:31219 same embryo
	EMAGE:31218 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source