EMAGE mouse gene expression spatial database

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EMAGE:31222

2610042L04Rik (2610042L04Rik)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003758_01	euxassay_003758_02	euxassay_003758_03	euxassay_003758_04	euxassay_003758_05

euxassay_003758_06	euxassay_003758_07	euxassay_003758_08	euxassay_003758_09	euxassay_003758_10

euxassay_003758_11	euxassay_003758_12	euxassay_003758_13	euxassay_003758_14	euxassay_003758_15

euxassay_003758_16	euxassay_003758_17	euxassay_003758_18	euxassay_003758_19	euxassay_003758_20

euxassay_003758_21	euxassay_003758_22	euxassay_003758_23	euxassay_003758_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 14 15 16 17 18

facial vii ganglion

weak

regional

weak expression: see section 21 22

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 18 19 moderate expression: see section 17

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

stomach

strong

regional

strong expression: see section 01 02 03 04 05 06 07 moderate expression: see section 08 09 10 11

rib

strong

regional

strong expression: see section 01 02 moderate expression: see section 03 23 24 weak expression: see section 05 06 20 21 22

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 08 18

cochlear duct

strong

regional

strong expression: see section 04 05 06 19 20 21 moderate expression: see section 07 08 09 16 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6972
Entity Detected:	2610042L04Rik, (2610042L04Rik)
Sequence:	sense strand is shown >T6972 GCCCTCTCTCAAGGAATACACCGTTAGAAAATATTGTCCATGTTTTGTACAATTAAACCTATAGGAGAAA GATATTTGCCTCTGTGAGACAGGAAAACCCTGGGAAATTGGAGTGTATGTCACATGAATTGACACAGCTG GTATATTTACTGTTGTGAAACATCTACTACATCCACAGTCCCTAGAGATTTGGGTGCTAACAAAATCTCT TAAGCATGTCAGAGTCCTAACCAACATTCCCCCTCAAAAGCACATGACATCTAATGAAGCAGTAGAATTC TCCAGCTTTGGAAGGAGAGGTTGCCAAAGGGGATCCCACGGGTCCATGAGAGGCTCCAGGAGAGGCACCC CAGAAAAGGACCAAGCTGGGCAGAACTAGTTAACAGCAGGAATGTTTTCTTGGCTGCTCAGGCTATTTCA GAAAGAGAATGGCGATGAAGGAGAGACCAGACCAACAGAGAAGGAAGAGGGAATCCTTTCTCATGAAAAA GGAAGAAGGAAATCATTCTGGAGAAGGCACAGGTCTGCTAGAAATACTTCAACCCAAAATTCCAAAATGA CTAAGAAGAGATCAAAAAGAAATGAACTAGAAGAACTGAAATTGGATATGAGGAAGATCAGCAATGACAT GGAGGAAATGTGTGGAATCCTGAACCTTTACATGTATGAGGATTTGAACTACAGGATGAACACTGAATTC AACATCATTAAATCACAACATGAGAAGACAATGTTGGATATGAATAAAATGATCCAGTCCATAATTGGTT CCATGCAGTATTCCAAGGAACTGATAGAAGATAACTATTCCTACAGCATTAAGGAGGACCACCTCCTCCG TGAGTGCACTCAACTCAACGAAAACATAAGGATATTACTGAATGAGAACAGAAGCCTGCTGGTGGAGCAG GCTGGCCATAAGTGTCCTGTGGGGAAGAAAAAAGGTTCTCTGAGGAGGCCAGCAAGAACATCTGTGTCCC AAGTGCCAAGGAACAGCAGTGTGATATAGTCCAACAGAAAGCAGAACATGGCACAGACCACGACATGATC TCCCTCAAAGAGAAGTGCTGGAGGAAGAGCACTGAGTGTGCACAGGAAATACACCACTGTTGCCTCTCAT CCCTAATAACCATGGCTGTAATGGGCTGTATGCTCCTCTTTTATTTTGTTTCTTTGGTATGAACAGGCCT TAATTTCATCTAGCCTCTGGCCCAGGAAGAGTGCACATTTAAAGGGACTCAGAGAAATGCTGAGACACAT CAAGAGCTGCTGGGCATCCAGGAAGAATCTGAGTGCAAATTTATCGTTTCCTGATGGGTCATCATCAATA ATTACATGGAGATCAGTCAACAAAATTGTAAAACCTTGGATCCAAGTCTACAACATGTGTTCTGCTTTGA CTTGGGAGGCCATATCCTTCAGACCCACACTCCAAAAGGAGAGTGATGCTTAAATTTCTCCTGCAAAGTT TGTTTCCTCCAGGAACTACTTTTCTACTAAGTTGCCAAGGACAGCCACAGGCTGTAAGTCTGTGCTACAA AATGAGCAGACTAAGAATTTTGCTTTGCACAATTTTTGTGGTTTGATTTTGGTTTGAGTTTTGATTAGTT TAGTTATTTGGTTTTTCTTGTTTTCATTCAAAGTTTTGTTATTTATTGGTTATTTATTGTTCTTTTAATT AATTTGATATTTTGATAAGGTTATACACAGTACATATTGACTGTCAGCTTTCAGTTACAATTGAGTACAT TGCATTTTTTCTTATGACTAACACAGTGATCTCCAACTCTTCACTCTAAGAGCCTTGTTATTTCAGGTGT GATCATGAAAGCCCACAGATATCAGACCCAGATGGATCTCTGCACTCTTCATGGGACTTGGGCTCCATAG TTTCTTCTGAGCCAGACTTAACTACAAAGTCCTTCATACATTCAGTATGGAGAGTTTGTCCAACTGTCTG GATAGGAACTTAATGATGGAAAACCTACCCATGCTGCATCGTTGCTGTCAAATATTTAGCTACTGTGAAA ATCCTGTGGATGATGGTGTTGAACGCATTAATGGCAAATACATCAGTTTTTCTGTAATAGCTCTCATTAA ATCAAAGCATAGTCTAAGGGAATAAAAAGCTGTCAGAAAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3987869 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3987869 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003758 same experiment
	EMAGE:31242 same embryo
	EMAGE:29412 same embryo
	EMAGE:31089 same embryo
	EMAGE:31188 same embryo
	EMAGE:29413 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	BrainStars same gene
	ViBrism same gene
Data Source