EMAGE mouse gene expression spatial database

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EMAGE:31233

Jagn1 ( MGI:1915017)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001570_01	euxassay_001570_02	euxassay_001570_03	euxassay_001570_04	euxassay_001570_05

euxassay_001570_06	euxassay_001570_07	euxassay_001570_08	euxassay_001570_09	euxassay_001570_10

euxassay_001570_11	euxassay_001570_12	euxassay_001570_13	euxassay_001570_14	euxassay_001570_15

euxassay_001570_16	euxassay_001570_17	euxassay_001570_18	euxassay_001570_19	euxassay_001570_20

euxassay_001570_21	euxassay_001570_22	euxassay_001570_23	euxassay_001570_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 14 15

submandibular gland primordium

weak

regional

weak expression: see section 05 06 07 08

basisphenoid bone

weak

regional

weak expression: see section 01 24

not examined

regional

not examined expression: see section 15

orbito-sphenoid

weak

regional

weak expression: see section 02 03 04 05 06 17 18 19 20 21 22 23

viscerocranium

weak

regional

weak expression: see section 06 07 08 12 16 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1318
Entity Detected:	Jagn1, ( MGI:1915017)
Sequence:	sense strand is shown >T1318 CCTCGAGACTGTTGGCCTACTGGCGGGCCGAGGTAGCAGGGAAGGGGTTCGCAGGGTGCTTGGCTGGGTG TGTGAGCAGAGGGTCGGCGTTAGGGGCCTTAGTTCTGTACAATGGCGTCTCGGGCAGGCCCGCGAGCGGC CGGCACCGACGGCAGCGACTTTCAGCACCGGGAGCGCGTCGCCATGCACTACCAGATGAGTGTGACCCTC AAGTACGAAATCAAGAAGCTGATCTACGTGCATCTCGTCATATGGCTGCTGTTGGTGGCCAAGATGAGCG TGGGACACCTGAGGCTCTTGTCACATGATCAGGTGGCCATGCCCTATCAGTGGGAATATCCGTATTTGTT GAGCATTGTGCCCTCTGTCTTGGGCCTGCTCTCCTTCCCTCGGAACAACATTAGCTACCTGGTGCTCTCC ATGATCAGCATGGGGCTCTTCTCCATCGCTCCCCTCATTTATGGCAGCATGGAGATGTTCCCTGCGGCAC AGCAACTCTACCGCCATGGCAAGGCCTATCGCTTCCTGTTTGGTTTTTCTGCTGTCTCTGTCATGTACCT GGNGTTGGTA
Notes:	The probe template was PCR amplified from IMAGE:2270146 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2270146 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001570 same experiment
	EMAGE:29441 same embryo
	EMAGE:31315 same embryo
	EMAGE:30368 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source