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EMAGE:31234

Pi4kb phosphatidylinositol 4-kinase, catalytic, beta polypeptide ( MGI:1334433)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31234
euxassay_003750_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
vertebral axis musculature
moderate moderate
regionalmoderate expression: see section 01
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6305
Entity Detected:Pi4kb, phosphatidylinositol 4-kinase, catalytic, beta polypeptide ( MGI:1334433)
Sequence:sense strand is shown

>T6305
AGCCACTTCTGAGCCTACTCCTAGCCCATCAGGGAATAATGGGGGCTCCCTACTAAGCGTCATCACGGAG
GGGGTCGGGGAACTGTCAGTGATTGACCCTGAGGTGGCCCAGAAGGCCTGCCAGGAGGTACTGGAGAAAG
TCAAGCTTTTGCATGGAGGTGTAGCCATCTCTAGCAAAGGCACCCCGCTGGAGTTGGTTAATGGGGATGG
TGTGGACAATGAAATCCGTTGCCTAGATGATCCACCTGCCCAGATCAGGGAGGAAGAAGATGAGATGGGG
GCTGGTGTGGCCTCTGGCACAGCCAAAGGAGCAAGACGACGACGACAGAACAACTCAGCCAAACAGTCTT
GGCTCCTGAGGCTGTTTGAGTCAAAACTATTTGACATCTCTATGGCTATTTCATACTTGTATAACTCCAA
GGAGCCTGGAGTGCAAGCCTACATTGGCAACCGGCTCTTCTACTTTCGCAATGAGGATGTGGACTTCTAT
TTGCCCCAGTTGCTTAACATGTATATCCACATGGATGAGGATGTGGGCGATGCCATTAAGCCCTACATAG
TCCACCGCTGTCGCCAGAGCATCAACTTTTCCCTCCAGTGTGCCCTGTTGCTTGGGGCCTACTCTTCAGA
CATGCACATTTCCACTCAGCGACACTCGCGAGGGACCAAGTTACGGAAGCTAATCCTCTCAG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers.. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_003750 same experiment
 EMAGE:31093 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS