EMAGE mouse gene expression spatial database

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EMAGE:31264

Senp5 SUMO/sentrin specific peptidase 5 ( MGI:2443596)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002886_01	euxassay_002886_02	euxassay_002886_03	euxassay_002886_04	euxassay_002886_05

euxassay_002886_06	euxassay_002886_07	euxassay_002886_08	euxassay_002886_09	euxassay_002886_10

euxassay_002886_11	euxassay_002886_12	euxassay_002886_13	euxassay_002886_14	euxassay_002886_15

euxassay_002886_16	euxassay_002886_17	euxassay_002886_18	euxassay_002886_19	euxassay_002886_20

euxassay_002886_21	euxassay_002886_22	euxassay_002886_23	euxassay_002886_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 16 17 18 19

facial vii ganglion

moderate

regional

moderate expression: see section 04

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

cervical ganglion

weak

regional

weak expression: see section 09 17

cervico-thoracic ganglion

weak

regional

weak expression: see section 11 17

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 18 19

thoracic ganglion

weak

regional

weak expression: see section 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T185
Entity Detected:	Senp5, SUMO/sentrin specific peptidase 5 ( MGI:2443596)
Sequence:	sense strand is shown >T185 CCACAAGCTTCAGACTTTCCCATGAAGTTCAGTGGGGAGAGCCAAAGTCCAGGTGACAGTGGCAAGACTG TGGTCTTGAACAAACATAGAAAGCGAGTTTGTCATGGCTGCTACCAAGGGCTAGAGCACCACAGGAACAG GAGACCCCTGATTCCAAAGCAGTTCCAGCTTAACCAACACCGAAGAGTCAGAGCGTCTCTGATGATGTAT GAGAAACTATCCATGATTAGATTTCGGTATAGGATTTTCAGATCCCAGCACTTCAGAACGAAAAGCAGAG TTTGCAAGCTAAGAAAGGCCCAGCGAAGTTGGGTACAGAAGGTCACTGGGGACCATCAAGAGAACCTTAG GGATAATAACACTGAGGGTGACAATTGCAATCCAGTCCCTTCCCTAGAACCTAAAGATCCTTGTCGGTGT CAGCCATACTTTCCAGACATGGACAGCAGTGCTGTGGGGAAGGGAAAAAACTGTCACGTGCCTGATGGTC ACACTA
Notes:	The probe template was PCR amplified from IMAGE:2647150 using vector specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2647150 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002886 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source