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EMAGE:31288

Pycard PYD and CARD domain containing ( MGI:1931465)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31288 EMAGE:31288 EMAGE:31288 EMAGE:31288 EMAGE:31288
euxassay_008184_01 euxassay_008184_02 euxassay_008184_03 euxassay_008184_04 euxassay_008184_05
EMAGE:31288 EMAGE:31288 EMAGE:31288 EMAGE:31288 EMAGE:31288
euxassay_008184_06 euxassay_008184_07 euxassay_008184_08 euxassay_008184_09 euxassay_008184_10
EMAGE:31288 EMAGE:31288 EMAGE:31288 EMAGE:31288 EMAGE:31288
euxassay_008184_11 euxassay_008184_12 euxassay_008184_13 euxassay_008184_14 euxassay_008184_15
EMAGE:31288 EMAGE:31288 EMAGE:31288 EMAGE:31288 EMAGE:31288
euxassay_008184_16 euxassay_008184_17 euxassay_008184_18 euxassay_008184_19 euxassay_008184_20
EMAGE:31288 EMAGE:31288 EMAGE:31288 EMAGE:31288
euxassay_008184_21 euxassay_008184_22 euxassay_008184_23 euxassay_008184_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
thymus primordium
weak weak
regionalweak expression: see section 12 13 14 15 16 17
heart ventricle
moderate moderate
spottedmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18
rectum
moderate moderate
regionalmoderate expression: see section 14
urethra of male
weak weak
regionalweak expression: see section 13 14 15
midgut
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17 18 19 20 weak expression: see section 08 09 10 11 12
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T198
Entity Detected:Pycard, PYD and CARD domain containing ( MGI:1931465)
Sequence:sense strand is shown

>T198
ATGCCATCCTGGACGCTCTTGAAAACTTGTCAGGGGATGAACTCAAAAAGTTCAAGATGAAGCTGCTGAC
AGTGCAACTGCGAGAAGGCTATGGGCGCATCCCACGCGGGGCCCTGCTGCAGATGGACGCCATAGATCTC
ACTGACAAACTTGTCAGCTACTATCTGGAGTCGTATGGCTTGGAGCTCACAATGACTGTGCTTAGAGACA
TGGGCTTACAGGAGCTGGCTGAGCAGCTGCAAACGACTAAAGAAGAGTCTGGAGCTGTGGCAGCTGCAGC
CAGTGTCCCTGCTCAGAGTACAGCCAGAACAGGACACTTTGTGGACCAGCACAGGCAAGCACTCATTGCC
AGGGTCACAGAAGTGGACGGAGTGCTGGATGCTTTGCATGGCAGTGTGCTGACTG
Notes:The probe template was PCR amplified from IMAGE:2648391 using vector specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2648391 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_008184 same experiment
 EMAGE:30539 same embryo
 EMAGE:29452 same embryo
 EMAGE:31279 same embryo
 EMAGE:31712 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS