EMAGE mouse gene expression spatial database

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EMAGE:31294

Flot1 ( MGI:1100500)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000221_01	euxassay_000221_02	euxassay_000221_03	euxassay_000221_04	euxassay_000221_05

euxassay_000221_06	euxassay_000221_07	euxassay_000221_08	euxassay_000221_09	euxassay_000221_10

euxassay_000221_11	euxassay_000221_12	euxassay_000221_13	euxassay_000221_14	euxassay_000221_15

euxassay_000221_16	euxassay_000221_17	euxassay_000221_18	euxassay_000221_19	euxassay_000221_20

euxassay_000221_21	euxassay_000221_22	euxassay_000221_23	euxassay_000221_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

strong

homogeneous

strong expression: see section 07 08 09 10 13 14 15 19 20 21 22 24

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 08 09 10 19 20 21 23 24

facial vii ganglion

strong

homogeneous

strong expression: see section 03 04 05 06 16 17

vagus x ganglion

strong

homogeneous

strong expression: see section 05 06 07 08 14 15

trigeminal v ganglion

strong

homogeneous

strong expression: see section 03 04 05 06 07 08 09 13 14 15 16 17 18

nasal cavity respiratory epithelium

moderate

single cell

moderate expression: see section 09

nasal cavity olfactory epithelium

moderate

single cell

moderate expression: see section 10 13 19 20 23 24

midbrain mantle layer

strong

regional

strong expression: see section 10

ventral grey horn

strong

homogeneous

strong expression: see section 10 13 19 20 22 23 24

vomeronasal organ epithelium

moderate

homogeneous

moderate expression: see section 20 23

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 06 14 15 16

pons mantle layer

strong

single cell

strong expression: see section 06 07 10 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T423
Entity Detected:	Flot1, ( MGI:1100500)
Sequence:	sense strand is shown >T423 GTCTGTTGCTGCAACAGGGTGCGGCCGGGTGGGGGGCGCAAGTGCAGCTTCCACTAAGCCGGGTGTCCGA GCAGTCCCCACCCCGTTCTGGGGTTCTGCGGGCCTCCGCGCTTCTCAAGACCCCGGTGGGGTTGCTTACC GGGACGTTCCGGAAGCTGCAGCTTCAACCATGTTTTTCACTTGTGGCCCAAATGAGGCCATGGTGGTCTC CGGGTTCTGCAGGAGCCCCCCAGTCATGGTGGCCGGAGGCCGAGTGTTTGTCCTACCCTGCATTCAGCAA ATCCAGAGGATCTCTCTCAACACACTGACCCTCAATGTCAAGAGTGAAAAGGTTTATACCCGCCACGGGG TCCCCATCTCAGTCACGGGCATTGCCCAGGTGAAAATCCAGGGCCAGAACNAGGAGATGCTGGCAGCTGC CTGCCAGATGTTCCTGGGGAAGACAGAGGCGGAGATTGCCCACATTGCCCTGGAGACACTGGAAGGCCA
Notes:	The probe template was PCR amplified from IMAGE:3158071 using vector specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3158071 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000221 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source