EMAGE mouse gene expression spatial database

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EMAGE:31299

Abhd11 abhydrolase domain containing 11 ( MGI:1916008)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000237_01	euxassay_000237_02	euxassay_000237_03	euxassay_000237_04	euxassay_000237_05

euxassay_000237_06	euxassay_000237_07	euxassay_000237_08	euxassay_000237_09	euxassay_000237_10

euxassay_000237_11	euxassay_000237_12	euxassay_000237_13	euxassay_000237_14	euxassay_000237_15

euxassay_000237_16	euxassay_000237_17	euxassay_000237_18	euxassay_000237_19	euxassay_000237_20

euxassay_000237_21	euxassay_000237_22	euxassay_000237_23	euxassay_000237_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 03 04

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 18 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 18 19 20 21 22 23

tail dorsal root ganglion

weak

regional

weak expression: see section 08 09 10 11 12 14 15 16 17 18 19 20 21 22

hindgut

strong

regional

strong expression: see section 15 16 17 18 19

foregut-midgut junction

strong

regional

strong expression: see section 14 15 16 17 18 19 20 21 22

anal canal

moderate

regional

moderate expression: see section 19

stomach

moderate

regional

moderate expression: see section 05 06 07 08 09 10

nasal septum

moderate

regional

moderate expression: see section 07 08 10 11 12 15 16 17 18 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07

midgut

strong

regional

strong expression: see section 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T208
Entity Detected:	Abhd11, abhydrolase domain containing 11 ( MGI:1916008)
Sequence:	sense strand is shown >T208 GTGGGCATGCTCCGCTGGGCGCGAGCGTGGAGGGTCCCCCGTGGGGTACTCGGTGCCTCCTCTCCCAGGC GTTTGGCGGTTCCTGTCACGTTCTGTAGCAGCCGCAGTAGTGGCCAAGAAAACGCCGATCTGAGGTCAGC CAAGGGCACTGGGAGGGTTGTTCTCCACTGGTCCACTTCGCACGCGGCTTAGCTGGCCCCGCCTCTCCTT TGGCCTCGCCCCTTTTTTCTTTCACTCTCTCCTCCCCCTTACTCCATGGTGGCCCCCACCTTCCAGGCCG CTGCCGCTGTCTTATAATCTTCTAGATGGAGACGCGACACTCCCAGCCATCGTCTTTTTGCATGGGCTCT TCGGCAGCAAAACCAACTTCAACTCCCTCGCCAAGGCAATGGTTCAGAGGACTGGCCGGAGGGTGCTGAC GGTGGATGCCCGGAACCACGGTGACAGCCCCCACAGTCCAGACGCAAGCTATGAG
Notes:	The probe template was PCR amplified from IMAGE:2648746 using vector specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2648746 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000237 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source