Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31301

Cobll1 ( MGI:2442894)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31301 EMAGE:31301 EMAGE:31301 EMAGE:31301 EMAGE:31301
euxassay_012201_01 euxassay_012201_02 euxassay_012201_03 euxassay_012201_04 euxassay_012201_05
EMAGE:31301 EMAGE:31301 EMAGE:31301 EMAGE:31301 EMAGE:31301
euxassay_012201_06 euxassay_012201_07 euxassay_012201_08 euxassay_012201_09 euxassay_012201_10
EMAGE:31301 EMAGE:31301 EMAGE:31301 EMAGE:31301 EMAGE:31301
euxassay_012201_11 euxassay_012201_12 euxassay_012201_13 euxassay_012201_14 euxassay_012201_15
EMAGE:31301 EMAGE:31301 EMAGE:31301 EMAGE:31301 EMAGE:31301
euxassay_012201_16 euxassay_012201_17 euxassay_012201_18 euxassay_012201_19 euxassay_012201_20
EMAGE:31301 EMAGE:31301 EMAGE:31301 EMAGE:31301
euxassay_012201_21 euxassay_012201_22 euxassay_012201_23 euxassay_012201_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
weak weak
regionalweak expression: see section 07 08 18 19
thymus primordium
weak weak
regionalweak expression: see section 11 12 13 14 15
stomach fundus
weak weak
regionalweak expression: see section 06 07
adrenal gland
moderate moderate
regionalmoderate expression: see section 09 10 11 17 18 19
vibrissa
weak weak
regionalweak expression: see section 06 07 20 21 22 23
hindgut
weak weak
regionalweak expression: see section 14
stomach
weak weak
regionalweak expression: see section 08 09 10
metanephros
weak weak
regionalweak expression: see section 08 09 10 11 12 17 18 19 20
testis
weak weak
regionalweak expression: see section 07 08 20 21
midgut
weak weak
regionalweak expression: see section 07 08 12 13 14 15 16 18
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37641
Entity Detected:Cobll1, ( MGI:2442894)
Sequence:sense strand is shown

>T37641
CAATGTCCCTTTGTCACCTGTAGATGGAAGTAATAAGAATCCAGCTGCTTCTTACCTAAAGAACTTTCCA
CTTTATAGGCAAGACTCCAACCCCAAGCCAAAACCGTCAAATGAAATCACGAGAGAGTATATACCTAAAA
TTGGAATGACTACTTATAAAATAGTACCTCCGAAATCCCTGGAAATGGCGAAAGACTGGGAATCAGAAGC
AATGGGGAGGAAAGATGACCAAAAGATGCTCCCTGTAGGGCAGAGGCATACGATTGAGAATATGACAGAA
ACTTCCATGCAAACAGAAGTCCCTGCTACTTCTAAAAGCTCACAGCAGCCTCAGCCTGACCTTAAACCAA
AGCCCAGCTCAGGAACAGAGCGTCATTTGCACAGGACTTTGAGCTCACCAACTGGTACCGAGACGAACCC
TCCCAAAGCACCCAGAGTGACTACAGACACTGGCACCATTCCTTTTGCGCCAAATTTGGAAGATATAAAC
AATATTTTGGAGTCAAAATTTAGATCCCGAGCTTCAAATCCCCAGGCCAAACCAAGTTCATTCTTTTTGC
AGATGCAAAAGAGAGCATCAGGCCACTATGTGACATCTGCAGCTGCTAAGAGCGTCCACACTGCCCCTGG
GCCTGCCCCTAAAGAGCCAACAATCAAGGAGGTGCAGAGGGACCCACAGCTTTCTCCAGAACAGCATCCT
TCTTCCTTAAGTGAAAGGACTCACTCTGCTCCGCTGCCCAATATTTCAAAAGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 95674. Forward Primer - name:095674_F_cDNA_Cobll1, sequence:CAATGTCCCTTTGTCACCTGTA; Reverse Primer - name:095674_N_SP6_cDNA_Cobll1, sequence:GCTTTTGAAATATTGGGCAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_012201 same experiment
 EMAGE:31208 same embryo
 EMAGE:31289 same embryo
 EMAGE:31232 same embryo
 EMAGE:31206 same embryo
 EMAGE:29459 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS