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EMAGE:31309

Ddx41 ( MGI:1920185)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31309 EMAGE:31309 EMAGE:31309 EMAGE:31309 EMAGE:31309
euxassay_000473_01 euxassay_000473_02 euxassay_000473_03 euxassay_000473_04 euxassay_000473_05
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euxassay_000473_06 euxassay_000473_07 euxassay_000473_08 euxassay_000473_09 euxassay_000473_10
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euxassay_000473_11 euxassay_000473_12 euxassay_000473_13 euxassay_000473_14 euxassay_000473_15
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euxassay_000473_16 euxassay_000473_17 euxassay_000473_18 euxassay_000473_19 euxassay_000473_20
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euxassay_000473_21 euxassay_000473_22 euxassay_000473_23 euxassay_000473_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
pancreas
moderate moderate
regionalmoderate expression: see section 09 10 11 15 16 17 weak expression: see section 12
testis
strong strong
spottedstrong expression: see section 09 10 11 15 16 17
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2204
Entity Detected:Ddx41, ( MGI:1920185)
Sequence:sense strand is shown

>T2204
TGGCCTCGAGCCAGATTCGGATCCCCAAGTGGTACCTTCTGCTTGCAGAACAGGTGGTACCTTCTGCTTG
GCCTCCAGCAACAAGGTACCACCTGTTCTGCAAGTGCTGCACTGTGGGGACGAGTCCATGCTGGACATTG
GAGGAGAACGGGGCTGTGCCTTCTGTGGAGGCCTTGGCCATCGGATCACTGACTGCCCCAAACTTGAAGC
TATGCAGACCAAGCAGGTCAGCAACATTGGCCGCAAGGACTACCTGGCCCACAGCTCCATGGACTTCTGA
GCCGTCTGACTTCTCTCTTCTCCCCAAGAGTCCAATGCTAGGACACTGGCCATGTGCACACCAGCACTCA
AGACAAGAAGCCAGCCTCCCGGCCCAGCTGGCCTGGGCAGCCCCAGTGCCCTCAGAATTACTATTTTTGT
TCTCCTCCCCAGCATTAAAGCACAATCCCTTTTCTTGGAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:920594 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:920594 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000473 same experiment
 EMAGE:30296 same embryo
 EMAGE:30278 same embryo
 EMAGE:31221 same embryo
 EMAGE:31236 same embryo
 EMAGE:30279 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS