EMAGE mouse gene expression spatial database

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EMAGE:31313

D5Ertd593e ( MGI:1261768)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000472_01	euxassay_000472_02	euxassay_000472_03	euxassay_000472_04	euxassay_000472_05

euxassay_000472_06	euxassay_000472_07	euxassay_000472_08	euxassay_000472_09	euxassay_000472_10

euxassay_000472_11	euxassay_000472_12	euxassay_000472_13	euxassay_000472_14	euxassay_000472_15

euxassay_000472_16	euxassay_000472_17	euxassay_000472_18	euxassay_000472_19	euxassay_000472_20

euxassay_000472_21	euxassay_000472_22	euxassay_000472_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

weak

regional

weak expression: see section 03 04 05 09 10 11 12 13 14 20 21 22 23

meckel's cartilage

moderate

regional

moderate expression: see section 22 23 weak expression: see section 04 11 12

lower jaw molar

weak

regional

weak expression: see section 13 14 16 20 21

upper jaw molar

weak

regional

weak expression: see section 13 14 16 20 21

ventral grey horn

weak

regional

weak expression: see section 02 15

chondrocranium

weak

regional

weak expression: see section 04 10 11 12 20 22 23

nasal capsule

weak

regional

weak expression: see section 01 02 08 14 15 16 17 18 20 21

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3813
Entity Detected:	D5Ertd593e, ( MGI:1261768)
Sequence:	sense strand is shown >T3813 TGGCCTCGAGGCCAGATTCGGCACGAGGNNTCAGCCCTGCTGGTCGGCGGGGGTCTAGCTGGAGCGCTCA TCCTGTGGCTGCTGCGGGGAGACTCTGGGGCCCCGGGGAAAGACGGGGTTGCGGAGCCGCCGCAGAAGGG CGCACCTCCTGGGGAGGCTGCGGCCCCGGGAGACGGTCCGGGTGGTGGTGGCAGTGGCGGCCTGAGCCCT GAACCTTCCGATCGGGAGCTGGTCTCCAAAGCAGAGCATCTTCGAGAAAGCAACGGACATTTGATTTCTG AGAGCAAAGATCTTGGTAACCTGCCGGAAGCACAGCGGCTGCAGAATGTTGGAGCAGACTGGGTCAATGC CAGAGAGTTTGTTCCTGTTGGGAAGATTCCAGACACACACTCCAGGGCCGACTCTGAAGCGGCAAGAAAT CAAAGCCCAGGATCTCATGGAGGAGAATGGAGACTCCCCAAAGGACAAGAAACAGCTGTCAAAGTAGCTG GCAGTGTGGCCGCAAAGCTGCCCTCCAGCAGCCTGCTTGTGGACAGAGCTA
Notes:	The probe template was PCR amplified from IMAGE:404084 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:404084 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000472 same experiment
	EMAGE:29706 same embryo
	EMAGE:30053 same embryo
	EMAGE:31300 same embryo
	EMAGE:29723 same embryo
	EMAGE:29646 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source