EMAGE mouse gene expression spatial database

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EMAGE:31346

Haus1 HAUS augmin-like complex, subunit 1 ( MGI:2385076)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003161_01	euxassay_003161_02	euxassay_003161_03	euxassay_003161_04	euxassay_003161_05

euxassay_003161_06	euxassay_003161_07	euxassay_003161_08	euxassay_003161_09	euxassay_003161_10

euxassay_003161_11	euxassay_003161_12	euxassay_003161_13	euxassay_003161_14	euxassay_003161_15

euxassay_003161_16	euxassay_003161_17	euxassay_003161_18	euxassay_003161_19	euxassay_003161_20

euxassay_003161_21	euxassay_003161_22	euxassay_003161_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 17 18 19 20 21

telencephalon ventricular layer

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 12 13 14 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4674
Entity Detected:	Haus1, HAUS augmin-like complex, subunit 1 ( MGI:2385076)
Sequence:	sense strand is shown >T4674 CTTTGAGATTGAGGTGGCGGGAGCGGCGGCTATGGCTGCGCCGGAGGAGAAAGCATTGCAGGTCGCTGAG TGGTTGAAAAAGGTATTTGGAGACCATCCCATCCCACAATATGAGATGAACTCACGGACGACAGAAATTT TATATCACCTTTCAGAACGCAACAGGGTCCGGGACAGGGACATCAGCCTGGTAATAGAGGACTTAAAACA GAAAGCAAGTGAATATGAATCCGAAGCCAAACGTCTTGAAGACTTTCTCATGGAGAGTGTGAATTTTTCA CCTGCCAATCTGTCTAAGTCTGGTTCCCGGTTTCTGAATGCACTGGTAGACAGTGCAATTGCCCTGGAAA TAAAAGATACTTCACTAGCAAGCTTCATCCCCGCAGTGAATGATTTGACGTCTGACCTTTTTCGTACCAA ATCCAAAAGCGAAGAAATGAAGCTTGAACTGGGAAAACTTGAAAAGAACCTGACGGCAACGCTGGTGTTA GAAAAATGTCTACGAGAAGATCTCAAGAAAGCAGAGCTGCAACTGTCTGCAGAAAAGGCCAAGGTTGACA GTCGCCTTCAGAATATGGACTTCCTAAAAGCGAAGGCAGCGGAGTTCCGGTTTGGAATCAAAGCTGCAGA GGAGCAACTTTCAGCCAGAGGCATGGATGCGTCTCTGTCTCATCGGTCACTAGCGGCACTTTCAGAGAAA CTCTCGGAGTTAAAAGAGCAGACTATACCCCTGAAGAAGAAGTTGGAGTCCTATTTAGATTTAATGCCGA GTCCATCTCTTGCTCAACTGAAAATTGAAGAGGCAAAGAGAGAATTAGACGCCATCGAGGCTGAACTTAC CAAGAAAGTAGACATGATGGGCCTGTGAGGACAGCCATGTAGACGTGGTCAGGGGAAGGAGGTGACTGTT CTTAGAGATGATGATGTCTCTATTAGTAAAACAACAGGGTTCGCTTCTGCCTTTAAACTTTGTGGCCTGA ATACAGTTTGCATATGAAAAGGCAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5026575 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5026575 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003161 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source