EMAGE mouse gene expression spatial database

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EMAGE:31375

Ddhd1 DDHD domain containing 1 ( MGI:2150302)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003133_01	euxassay_003133_02	euxassay_003133_03	euxassay_003133_04	euxassay_003133_05

euxassay_003133_06	euxassay_003133_07	euxassay_003133_08	euxassay_003133_09	euxassay_003133_10

euxassay_003133_11	euxassay_003133_12	euxassay_003133_13	euxassay_003133_14	euxassay_003133_15

euxassay_003133_16	euxassay_003133_17	euxassay_003133_18	euxassay_003133_19	euxassay_003133_20

euxassay_003133_21	euxassay_003133_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex marginal layer

moderate

homogeneous

moderate expression: see section 10 11 15 16 17 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 18 19

cervico-thoracic ganglion

weak

regional

weak expression: see section 10 17

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 18 19

thoracic ganglion

weak

regional

weak expression: see section 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 15 16 17 18

facial vii ganglion

moderate

regional

moderate expression: see section 04 21

lower jaw molar

moderate

regional

moderate expression: see section 06 07 20

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 10 11 16 17 18 19

cerebral cortex marginal layer

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 11 14 15 16 17 18 19 20

upper jaw molar

moderate

regional

moderate expression: see section 06 07 20

thymus primordium

moderate

homogeneous

moderate expression: see section 11 12 13 14 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22

vibrissa

weak

regional

weak expression: see section 05 06 21

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 08 09 10 11 12 15 16 17 18 19

cervical ganglion

weak

regional

weak expression: see section 09 17

naris

weak

regional

weak expression: see section 12 16

liver lobe

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5399
Entity Detected:	Ddhd1, DDHD domain containing 1 ( MGI:2150302)
Sequence:	sense strand is shown >T5399 ATTGTCTGTCACTGGTAAATTTAGATATTATTTGTCTTTGTTCCTTTCAATAAAAGTAGAGCATATATAT TTGACAGACATTAACTAATTTTTGCTTTACTGGTCTTTTAACTAGGAGTTGCATTTTAGAAAGCATTTAG AATAATGTTTGTCAAGTGCAGTTTCTAAAAGTTAGGGCTAAAAGGCAGAGGCAGAATAGAAAACCTCACT TAAATTTCCTTTGAAGCTCAGTGTTTTTGCTTTCTTAGTTGTCTACCACCTCCCTCCCTTCTCCCTCCCT TCTCTGTTCAAATGGAGAGGATATAAGGAGAGCACAGATGCCACAGGTCTTAAGCGAAAGCGTTCCCAAG CTGTTCACAGTTTCAAGCTGAGTCGGAACCACGTGGACTGGCACAGCATGGATGAAGTCTATCTCTACAG TGACGCAACAACATCCAAAATTGCGAGAACCGTTACCCAAAAACTGGGCTTTTCTAAAGCCTCCAGCAGT GGGACCAGACTGCACAGAGGTTATGTAGAAGAAGCCACACTGGAAGACAAGCCGTCGCAGACTTCTCATA TCGTGTTTGTTGTGCACGGCATTGGACAGAAGATGGACCAAGGAAGAATCATCAAAAACACAGCCATGAT GAGAGAGGCTGCAAGGAAAATGGAAGAAAAGCATTTTTCCAACCATGCAACACATGTTGAATTTCTGCCT GTTGAATGGCGATCAAAGCTTACTCTTGATGGAGACACAGTTGATTCCATCACTCCTGACAAAGTGCGAG GCCTGAGGGACATGCTCAACAGCAGCGCGATGGACATCATGTACTACACCAGCCCACTCTACAGGGATGA GCTAGTTAAAGGCCTTCAGCAAGAGCTGAATCGATTATATTCCCTTTTCTGTTCCCGGAATCCAGACTTT GAAGAGAAAGGGGGTAAAGTCTCAATAGTGTCCCATTCCTTGGGGTGTGTAATCACTTATGACATCATGA TGGGCTGGAATCCAGGTGGACTCTATGAGCAGCTGCTGCAGAAGGAGGAAGAGCTGCCCGACGAGCGGTG GATGAGCTATGAAGAGCGGCATCTTCTTGACGAGCTCTATATAACAAAGCGACGGCTGAGGGAAATAGAA GATCGGCTGCACGGATTGAAGGCGCCATCCATATCACAAACACCTGCCTTAAAATTTAAGGTTGAAAATT TCTTCTGTATGGGATCTCCGCTAGCAGTTTTTTTGGCATTACGCGGCATCCGACCAGGAAATTCTGGAAG TCAAGACCACATTTTACCTAGAGAGATCTGTAACCGCTTACTAAATATTTTTCATCCTACGGACCCAGTG GCCTACAGATTAGAACCGTTGATTTTGAAACACTACAGCAACATTTCTCCAGTCCAGATCCACTGGTACA ACACTTCAAACCCTCTACCTTATGAGCATATGAAGCCAAATTTTCTCAACCCAGCAAAAGAACCTACCTC AGTTTCAGACAGTGAGAACATAGCAGCTATCCCAAGCCCTGTGACCTCCCCCGTCTTATCTCGGCGCCAC TATGGAGAATCTATAACTAACATTGGCAAAGCAAGCATATTGGGGGCTGCTAGCATTGGAAAGGGCCTTG GAGGAATGTTGTTCTCAAGATTTGGACGTTCTTCAGCATCACAGCCGTCTGAGCCATCTAAAGACTCACT GGAAGATGATAAGAAGCCTTCAGCTTCACCCTCTACCACCACCGTGGCAACGCAGACCCTGCCGCACAGT GGCTCCGGCTTCCTCGACTCCGCATATTTCAGACTTCAAGAATCGTTCTTTTATCTCCCACAACTTCTTT TTCCGGAAAATGTAATGCAGAGTAAAGATGATAGCCTCGTGGAACTGGAACACAGGATCGACTTTGAACT CAGAGAAGGCCTTGTGGAGAGCCGCTATTGGTCAGCCGTCACGTCGCACACTGCCTATTGGTCATCCTTG GATGTCGCTCTTTTTCTTTTAACATTCATGTACAAACACGAGCATGATACCGAGGCCAAGCCCAGCCTAG GTTCACTCTGAACTCTTGAAGGACGTGAATGCCCAAAGCTTTTCTGTTAAACATGTGTCAAGATGCAGAG ACTTCAAGGTTCCAGTTTTGTTAAGGGCAAGAACTATTTTAATTTAAAAGCACTTTATTTTATTTTTAAA AGAGAAAAAAACCACATGTTCAGTTCTAAAGGAGTTATTTATGTTTCTGTCGTTTTGATTCTGTCTGGCA GAGCCCTGCAAAGAATCACCAGAGTAGAAGTGTGGTGGGCTTAGGAGAACTGCATGAAGGCAGCCATCAC AGTCCAGGTCTCCAGAGGAGCCTAATGTAGCGTTTTAACCAAAAGGCGTCACAGCACTAACAGCCCTAAG TCCAGTACACTCTGCATAGACTACAAGGTGGCATCATGGTTTCTTGATTCTGTGAAGGCTTCCGTGTCCG CAGTTCCTTTGAAAGAAGAGTATAACAAATTTTAAATGTTCTAAATTTAACTTGTTTTGAAAAACTAATG CTAAAAAGCAATATTTGAACTACTGCACTTATAATTTATAATCTCTACTTATTCAGCATATGAAACATTT TTAATATTTCTTTTAAGCCACACTTATTTTGTATCATGTTGAGGCCCCTTTTTCTCAAAACTCCATCTTT GTAAATGAGCAGATGATCCTGTGACGTACTTTCAGGGTTTTTTTGTTTGTTTGTTTTGGTTTGGTTTTTT CTTTTTGTTTTTTTTGTTGTTGTTTTATTTTTGTTTTCTTTTCTTTTTCATTAAAAACATGGTTTCACAC AGAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5365658 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5365658 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003133 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source