EMAGE mouse gene expression spatial database

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EMAGE:31394

Timp4 ( MGI:109125)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006892_01	euxassay_006892_02	euxassay_006892_03	euxassay_006892_04	euxassay_006892_05

euxassay_006892_06	euxassay_006892_07	euxassay_006892_08	euxassay_006892_09	euxassay_006892_10

euxassay_006892_11	euxassay_006892_12	euxassay_006892_13	euxassay_006892_14	euxassay_006892_15

euxassay_006892_16	euxassay_006892_17	euxassay_006892_18	euxassay_006892_19	euxassay_006892_20

euxassay_006892_21	euxassay_006892_22	euxassay_006892_23	euxassay_006892_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

midbrain ventricular layer

strong

regional

strong expression: see section 11 12 13 14 15 moderate expression: see section 10 16

hypothalamus ventricular layer

strong

regional

strong expression: see section 12 moderate expression: see section 11

ventral grey horn

strong

single cell

strong expression: see section 12 13 14 15 16 weak expression: see section 17

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 11 12 13 14 15

not examined

regional

not examined expression: see section 04 05 06

pons ventricular layer

strong

regional

strong expression: see section 07 08 11 13 14 15 moderate expression: see section 09 10 16 17 18

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 04 05 06 07 08 11 12 14 15 19 20 21 moderate expression: see section 09 10 16 17 18

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12 moderate expression: see section 11 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8190
Entity Detected:	Timp4, ( MGI:109125)
Sequence:	sense strand is shown >T8190 GTTTGTTACGACAGGCAGACCCCACGAGCTCAGCTGGGGCTCTGCAGTGTCATGCCCTGGAGTCCCCTGG CTGCGCTGAGCTGGGCGCTGGTGCTGAGGCTGCTGGCTTTGCTGTGGCCCCCTGGACGGGGTGAGGCGTG CAGCTGTGCGCCTGCGCACCCCCAGCAGCACTTCTGCCACTCGGCTCTAGTGATACGGGCCAAAATATCC AGTGAGAAGGTAGTCCCTGCCAGCAAAGACCCTGCTGACACTCAAAAACTGATCCGGTATGAAATCAAAC AGATAAAGATGTTTAAAGGGTTCGAGAAGGCCAAGGATATTCAGTATGTCTACACGCCATTTGACTCTTC CCTCTGTGGTGTGAAGCTAGAAACCAACAGTCACAAGCAGTATCTTTTGACTGGCCAGATTCTCAGTGAT GGAAAAGTCTTCATCCATCTGTGCAACTACATTGAGCCCTGGGAGGACCTGTCCTTGGTGCAGAGGGAGA GCCTGAATCATCACTACCACCAGAACTGTGGCTGCCAAATCACCACTTGCTATGCAGTGCCATGTACCAT CTCAGCCCCCAATGAGTGTCTCTGGACGGACTGGCTGCTGGAACGGAAGCTCTATGGGTACCAGGCCCAG CACTATGTCTGCATGAAGCATGTTGATGGCATCTGCAGCTGGTACCGAGGCCACCTACACCTCCGGAAGG AGTACGTTGACATCATCCAGCCCTAGTAGGGACCAGGAACCACATCCTTGGAAGCATTCTGAAGACCAAG CCAGTTCTCTGTGGTCCTTTGACCATCACCACCTGCTGTGTTGCTGCCAGATAATGGAAAATGACAAGTG GATGGCCTGGCCAGCAAATGGAGGCTGTGGGGCATGTGACAGCTTGTGTCTGCTGTTCACATCTCCAGCT CACAACTTGTCAAGGGTTGGAAAGGTATCTTATCTGCCTTGCCCTCAGCCCCTCTTCCCTGTCTCCCAAA CCCTGTTAGTCTAGCTTATACCTATTACTGAGAGTTTTTATTCTGGCTTTGTTTAACTGGCTTTTCTTAG CTCATATGATAAGGGAGAAGCTGTGACTGACTGTCATAAAGTTGTACAACCTTGCGATGTGTGCTATGGT AGGTGGGCTGACTGTATCCTGAGTGGGCTGTTTGGCTGAAGTGAAAAGAACACACTAAAGCTCCAGATCT CCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:875422 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:875422 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006892 same experiment
	EMAGE:29384 same embryo
	EMAGE:31422 same embryo
	EMAGE:31453 same embryo
	EMAGE:31445 same embryo
	EMAGE:31434 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source