EMAGE mouse gene expression spatial database

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EMAGE:31397

Rhov ( MGI:2444227)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002055_01	euxassay_002055_02	euxassay_002055_03	euxassay_002055_04	euxassay_002055_05

euxassay_002055_06	euxassay_002055_07	euxassay_002055_08	euxassay_002055_09	euxassay_002055_10

euxassay_002055_11	euxassay_002055_12	euxassay_002055_13	euxassay_002055_14	euxassay_002055_15

euxassay_002055_16	euxassay_002055_17	euxassay_002055_18	euxassay_002055_19	euxassay_002055_20

euxassay_002055_21	euxassay_002055_22	euxassay_002055_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

oral epithelium

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

spinal cord

weak

homogeneous

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13

rectum

strong

regional

strong expression: see section 12 13

urethra of male

strong

regional

strong expression: see section 11 12

brain

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

bladder

moderate

regional

moderate expression: see section 09 10 11

esophagus

moderate

regional

moderate expression: see section 05 06

pancreas

moderate

regional

moderate expression: see section 07 08 11 weak expression: see section 12

submandibular gland primordium

moderate

regional

moderate expression: see section 03 05 11 12 13 14 weak expression: see section 04

thymus primordium

weak

regional

weak expression: see section 05 06 07 08

nasal cavity respiratory epithelium

weak

regional

weak expression: see section 12 15

vibrissa

moderate

regional

moderate expression: see section 05 06 07 19 20 weak expression: see section 04

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 08 09 10 11 12 14 15 17 18

epidermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

naris

moderate

regional

moderate expression: see section 13 14 weak expression: see section 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4616
Entity Detected:	Rhov, ( MGI:2444227)
Sequence:	sense strand is shown >T4616 CCACGCGTCCGCTTGGACAGATTGTCAGCGTCCGGCTGCGGAACCTGCTTCCCCGAGTGCCGCGGAGCCT GGTCCGGGCTGGCCCCTCTGCTACCCCAGGAGCGGACCATGCCGCCGCGGGAGCTAAGCGAGGCCGAGCC ACCGCCTCTCCCGGCCTCGACCCCTCCTCCGCGGCGGCGCAGCGCCCCTCCGGAGCTGGGCATCAAATGC GTGCTGGTGGGCGATGGCGCGGTGGGCAAGAGCAGCCTCATCGTCAGCTACACCTGCAATGGGTACCCCG CGCGCTATCGGCCTACAGCACTAGACACCTTCTCCGTGCAAGTCCTGGTCGATGGAGCCCCTGTGCGAAT CGAGCTTTGGGACACCGCAGGGCAGGAGGACTTTGACCGGCTTCGTTCTCTCTGCTACCCGGACACCGAT GTCTTTCTGGCTTGCTTCAGCGTGGTGCAGCCCAGCTCCTTCCAAAACATAACAGAAAAATGGCTGCCTG AGATCCGCACTCACAACCCCCAAGCACCCGTGTTGCTGGTGGGCACTCAGGCTGACCTAAGGGACGATGT CAATGTACTAATTCAGTTGGACCAAGGAGGCCGGGAGGGCCCAGTACCACAACCCCAAGCCCAGGGTCTG GCTGAGAAGATCCGCGCCTGCTGCTACCTTGAGTGCTCGGCCTTGACGCAGAAGAACTTGAAGGAGGTGT TCGACTCGGCCATTCTCAGTGCGATTGAGCACAAAGCCCGCCTGGAGAAGAAACTGAATGCAAAAGGTGT GCGCACGCTGTCTCGCTGTCGCTGGAAGAAGTTCTTCTGCTTTGTGTGAGCAGCTATGGCAAGAGAGAGG CGGGTGGCCTGAGACTTCTGGACCCCGAGACATCGGGTACTGGCAGGGCCTGGCCAACCCCTGGAACTCA GTTCTCTATTGAACACAGGGCACATGGGCCTCAAAGCTGAACACCCTGGTGAGCCAGGGTGAGCTCTGTC TGTGCAGGGGCTGCCCCATTTGGATTTCTTTGGTCAAGACTCACAGGAAAATCCCAGCACTTTGATTTTC ATGGGATAGCGCCATCAGCGTCAGTGCTGCTGAGCAGCTTGGGATGTAATTCTCAGTTTCTTATCCTGGG GCCACAGGTCAGTTTGGCTGAATGCAGGCCCCTCGGGGTCATCCCCCTCTCCTAGCACAGTGCGAGGATC AAGGAGAACAGTGGGGCATCTTGCAGGGCTGGCGGATGGTCAGCTTGATTTGGAGACGTTTGTGATGGGG TATGAAGAAGAGGGCAGTGAGAGATTGAGAGGGAAAACAGAAAGTGAGGTGAGTGGTGGACAGGAGGCAG ATTAGAGGCCTGGGCCTGGAAGGAGGAAGACAGGAAGAGAGAAGGGTGGACAGCTGTGGAGAAGGCCGAC ACCTGGGCTGCCCTCGAGCCCCAAGGCCAGGGAGGACAGGACTGCTCCCTGGGAAGAACTGGGTCTCAGG GTTCCCCAGGCACTGGCCAAACTGGCTGGGCTAGCAGAGGGGCAGGAAGTGAGAGTTAAGGCCCAGCAAA GGGAGGGGAGGAGCTGCAGGTAGGAGCCTGAAGGAGGAAGGGACAGGGACCGTCCCTTTCCCCAAGGTCA TAGCTTAGAAGGTGAGCTATACAGTCTGCAAATAAAACCTTCCATTTCTCAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5003249 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5003249 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002055 same experiment
	EMAGE:29490 same embryo
	EMAGE:31391 same embryo
	EMAGE:31374 same embryo
	EMAGE:31364 same embryo
	EMAGE:29503 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source