EMAGE mouse gene expression spatial database

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EMAGE:31400

Tmprss4 transmembrane protease, serine 4 ( MGI:2384877)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003119_01	euxassay_003119_02	euxassay_003119_03	euxassay_003119_04	euxassay_003119_05

euxassay_003119_06	euxassay_003119_07	euxassay_003119_08	euxassay_003119_09	euxassay_003119_10

euxassay_003119_11	euxassay_003119_12	euxassay_003119_13	euxassay_003119_14	euxassay_003119_15

euxassay_003119_16	euxassay_003119_17	euxassay_003119_18	euxassay_003119_19	euxassay_003119_20

euxassay_003119_21	euxassay_003119_22	euxassay_003119_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 11 12 14 15 16 17 18 19

hindgut

moderate

regional

moderate expression: see section 11 12 13 14 15 16

foregut-midgut junction

moderate

regional

moderate expression: see section 12 16

stomach

strong

regional

strong expression: see section 04 05 06 07 08 09 moderate expression: see section 10

rectum

strong

regional

strong expression: see section 14 moderate expression: see section 13

midgut

strong

regional

strong expression: see section 05 06 07 08 09 moderate expression: see section 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4698
Entity Detected:	Tmprss4, transmembrane protease, serine 4 ( MGI:2384877)
Sequence:	sense strand is shown >T4698 CCACGCGTCCGGGCAGGTGGTAGCTGGCTTGCGGAAGGAAGAGGAAGAAGAGAATGTGGGCCATCAAGGA GCAAGGCCAGCCTTGCACTTGGGCCCCCTCTGCTCAGTGCTGACCAGGGCTTTCTGAGCCGCTTCCTAAT GAGGCTCATTTGAAGACCCCCCCACCCCCCTCCTGCTGTCTTGGGTGGCAGAGCTAGCTCCAGGCTGTAA GAAAATTAGGAGGATTACCAAAGCAGTATGGAGTCAGACAGTGGCCAACCCCTCAACAACCGTGATATTG TTCCCTTTCGCAAACCCCGAAGGCCCCAGGAGACCTTCAAAAAGGTGGGGATCCCCATCATTGCAGTGCT GCTGAGCCTGATAGCCCTCGTGATTGTGGCCCTTCTCATCAAGGTGATTCTGGATAAATACTACTTCATC TGCGGCAGTCCCCTGACCTTCATTCAGAGGGGCCAGTTGTGTGACGGCCACCTTGACTGCGCCTCAGGGG AGGATGAGGAACACTGTGTCAAGGACTTCCCTGAAAAGCCCGGAGTGGCAGTCCGGCTCTCCAAGGACAG ATCCACCCTGCAGGTGCTGGATGCAGCCACAGGGACCTGGGCCTCAGTCTGTTTCGACAACTTCACAGAA GCACTGGCCAAGACAGCCTGCAGACAGATGGGCTATGACAGCCAGCCCGCTTTCAGAGCAGTGGAGATCC GTCCAGATCAGAACCTCCCTGTTGCTCAAGTCACAGGAAACAGCCAGGAACTTCAGGTGCAGAATGGAAG CAGATCCTGCCTCTCAGGCTCCCTGGTTTCCTTGCGCTGCCTTGACTGTGGAAAGAGCCTGAAGACTCCT CGTGTGGTGGGTGGGGTGGAGGCCCCTGTGGATTCTTGGCCGTGGCAGGTCAGCATCCAGTACAACAAGC AGCATGTCTGTGGTGGGAGCATCCTGGATCCCCACTGGATCCTCACAGCAGCCCACTGCTTCAGGAAGTA TCTTGATGTGTCAAGCTGGAAGGTCAGGGCAGGCTCAAACATACTGGGTAACTCTCCATCCTTGCCTGTG GCCAAGATCTTCATCGCTGAACCCAATCCTCTGTACCCCAAAGAGAAGGACATTGCCCTTGTTAAGCTGC AGATGCCACTCACATTCTCAGGCTCAGTCAGGCCCATCTGCCTGCCCTTCTCTGATGAGGTGCTTGTCCC AGCCACACCAGTCTGGGTCATTGGATGGGGCTTTACAGAAGAAAACGGAGGAAAGATGTCTGACATGCTA CTGCAGGCATCAGTCCAGGTCATCGACAGCACACGGTGCAATGCAGAGGATGCCTACGAAGGGGAAGTGA CCGCTGAGATGCTGTGTGCAGGTACCCCACAGGGTGGCAAGGACACCTGCCAGGGTGACAGTGGTGGGCC TTTGATGTACCATTCTGACAAGTGGCAGGTAGTAGGCATCGTGAGCTGGGGCCATGGATGCGGCGGCCCA AGTACTCCTGGAGTGTATACCAAGGTCACTGCCTATCTCAACTGGATCTACAATGTTCGGAAGTCTGAGA TGTAACGCTGCCGTCCCCCACATCCAGAAGCTGCTTCCCTTCAGACCTACCTACGGCATGACCCCTCAAA GTCAGATATGGGACAAGAGCCTCCTTGAACAAACTCTGGTATCCCTGCAGCAAGCAAGGATACATTGCAG AGGTGCCCGGAGTGGAGTCAGATGGGCTAGCTCAGCCACCCCTGCATCTCCCAAACCCTGGGAGACATGT GGCCCATGGGAGTAAATCCAGGACATTGACTCAACTCTCAGAAGTGTTATTCAGTCAAGGAGGCTCTCCC TTCCACTGAAGGAAGGAAAGTCAGCTCTCTCCTGAAAGGCCAGATCACTGGCTGAGTAGATGAGACAAGG GTATGAAAGGCCTTTGCCATCTTCTTTGCCCAGTCCTGAAAGCACTGACGTAAGAGACCAGTCAGTTCTA ATGTAAGGTGTATATTTTAGTCTCAGGGTATTGCAATTGTCACCCCTGTGGTCAATATCATTAAACAGGT ATGAGAATTCGCTGGCATAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5030266 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5030266 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003119 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source