EMAGE mouse gene expression spatial database

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EMAGE:31416

Elmod3 ELMO/CED-12 domain containing 3 ( MGI:2445168)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_003113_11	euxassay_003113_12	euxassay_003113_13	euxassay_003113_14	euxassay_003113_15

euxassay_003113_16	euxassay_003113_17	euxassay_003113_18	euxassay_003113_19	euxassay_003113_20

euxassay_003113_21	euxassay_003113_22	euxassay_003113_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 12 13 14 16 17 18 19 20 moderate expression: see section 10 11

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4182
Entity Detected:	Elmod3, ELMO/CED-12 domain containing 3 ( MGI:2445168)
Sequence:	sense strand is shown >T4182 CCCACCTGAACATGAGTCATGAGTGAAACATCTTGCTCTTTTTTTATTGAGAAAGAATTTCAAGATGGGC AGCTAGAAAATGTCTCTGCTGGGCTCTCTTCATCGTACAAGGACAAGGGTGCACTCATGGCTTTCAGAGG AATTCCTATCTCCGAGCTGACGAACCATGGCATTCTCCAGGCCCTGACGGCAGAAACCAATGGGTGGCAG CCAGGTGTTGTGAGTGAAGAGGTCCTCAGAGCCCAGGAAGAGTGGGAAGTGGTGGACACCATCCATCCAG ACATAGAGAGTGGAGTCCACTGCCAGCAGCCTGGCCAGCTCATCTCCTTCAACGAGGCCCTGGAGCACTT CCAGAGTGTGGACCTTTCCTCTTTCAAGAAAAGAATCCAGCCCACTATTCAAAGGACTGGGCTCGCCGCC CTCCGGCATTGCCTTTTCGGGCCGCCAAAGCTGCACCAGGGTCTTCGGGAAGAAAGAGACTTGGTTCTCA CCATTGCTCAGTGTGGCCTGGACAGCCAAAACCCAACGCATGGCCGAGTGCTCCAGACCATCTACAAGAA GCTGACTGGCTCCAAGTTCGACTGTGCCCTTCACGGAGACCATTGGGAAGACCTGGGCTTCCAGGGAGCA AACCCAGCCACAGACCTCCGGGGGGCAGGCTTCCTGGCCCTCCTGCACCTACTCTACCTGGTCATGGACT CAAAGACGTTTCTGATGGCCCAGGAGATCTTCCGCCTGTCTCATCACCATATCCAGCAATTCCCCTTCTG TTTGATGTCTGTGAACATCACCCGCATTGCCATCCAGGCCTTGAGAGAGGAGTGTCTCTCCAGGGAGTGT AATCGGCGGCAGAAGGTCATCCCGGTGGTGAACAGCTTCTACGCGGCTACTTTCCTTCATCTGGCTCGCG TGTGGAGGACCCAGCAGAAGACTATTTTAGACTCAGGCTTTGTCCTCAAAGACTTGGAAGCGTTGGCCAA GAAGAGCCCAAAACGACTGCTCAAGACCCTGGATGACTACCTCGCCCAGGTGTCAAAGGGACAGACCTCC TTGTTGGAGGCACACAAGCGCCCTGGATCACAGGGACCTCACTCCAGGGATCTCACTTTCACAGGCGTGT GTGACCTGCAGTCTCACTCATTCGAGAGTGCAGGGTTGATCTGACCCACCACGGAGAGACCAGGACAGGC TGGGTCGCTGGGGCTCTCTGAACTAAAGAATCATGTCTGGGCCAAAGCCCTTCGTGCCCCTGCAGATGAG ACTAGGAGGCTCCCGGGTGTGTGTAGTGACACCCACGCCCACATTTCTACATCAGCAGAGCACATTCAGG CAGCTAGAATTACCCCCCCATGCCGTCCTCCAAGCACGGAGTCTTCTGTCCTTCCCAGCTGGACTCCACT TCACAGGGTTGGACTCCTGATATCTCAGTGGAACTGAACAGAGCACAGGGCCAGACAGCCCTATCAGAAT TGCCTACACGTCTGCACCTAGAGGCAGAGACCCTGGAAGCAACAGCCAAGATTCTGACTCCCAACAGCTG TCCCCAGTGTCTGTGGTTGGAATTGGAAGGACAGAGACAGCAATATCTTCAGCCATCAGGGGGCAGCACA ACACAGAAAAAGTGACTGATAGGCAGGTCCTCTCTTCCTCCAGCCACCGATTTTTCAAGACACTTGTTTC CACCCTACCCTTCTCCTCTTTCCCCTCCCTCTCCTTTGCCCACCATTGTTTGTAATCTAGAAATGGGTAC TCGGCTGGCTAGACTAGTCATTGAGCGTCCCACCCCCAACGCCCACAGGCTGGAGCGCCTATGGAGACCA CTACCTATCTACCCTGGCTGAGAGGCAAAGGAAACTCACCCACCAGCAGTTTTAGAATAAAGGAATTGTT GTAATCTCTGAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4487200 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4487200 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003113 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source