EMAGE mouse gene expression spatial database

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EMAGE:31421

Mboat7 membrane bound O-acyltransferase domain containing 7 ( MGI:1924832)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_003110_16	euxassay_003110_17	euxassay_003110_18	euxassay_003110_19	euxassay_003110_20

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View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 10 16 17 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 weak expression: see section 06

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4689
Entity Detected:	Mboat7, membrane bound O-acyltransferase domain containing 7 ( MGI:1924832)
Sequence:	sense strand is shown >T4689 GGGACCTCCTAGAAAGATCGCGACAGGCATCTTGCGGCAGACTGGTGTTCTGGGTACCTTTCTTGCGGTG CTGTAACTCGTACAGCCGCGGCTCTCGGGGCCTGGACCGCGCAGCCCTGCCGGCGCCGTCCAGAACGGGC AGTGCGGGGGCGTGCTGAGCTGGGGAGGCGTGGCGCGAGCCGAGGCGGCCTCGAAAAGGAGCTCCGCAGT TTTCTGGCCCACGGACGGTTCAGACCATGACACCCGAAGAATGGACATATCTAATGGTCCTTCTTATCTC CATCCCTGTTGGCTTCCTCTTTAAGAAAGCTGGACCTGGGCTGAAGAGATGGGGGGCAGCAGCTGTGGGC CTGGGGCTCACCTTATTCACCTGTGGCCCCCACAGTTTGCATTCTCTGATCACCATCTTGGGAACCTGGG CCCTCATTCAGGCCCAGCCCTGCTCCTGCCATGCCCTGGCTCTTGCCTGGACCTTCTCCTATCTCCTCTT CTTCCGAGCCCTCAGCCTGCTGGGCCTGCCCACTCCCACGCCCTTCACCAATGCTGTCCAGCTGCTGTTG ACACTGAAGTTGGTGAGTCTAGCTAGTGAAGTCCAGGATCTGCATCTGGCTCAGAGAAAGGAAATAGCCT CCGGCTTCCACAAGGAGCCTACGCTGGGCCTCCTCCCTGAGGTCCCCTCTTTGATGGAGACACTCAGCTA TAGCTACTGTTACGTGGGAATCATGACAGGCCCATTCTTCCGCTACCGCACCTACCTGGATTGGCTGGAA CAGCCCTTCCCGGAAGCCGTGCCCAGCCTGAGGCCCCTGCTGCGCCGCGCCTGGCCAGCCCCGCTCTTTG GCCTGCTCTTCCTGCTGTCCTCCCATCTCTTCCCACTGGAAGCTGTGCGTGAGGACGCCTTCTACGCCCG CCCGCTGCCCACCCGCCTCTTCTACATGATCCCGGTCTTCTTCGCCTTCCGCATGCGCTTCTACGTTGCC TGGATTGCGGCCGAGTGCGGTTGCATTGCCGCGGGCTTCGGGGCCTACCCTGTGGCTGCCAAAGCCCGGG CCGGGGGCGGCCCCACCCTCCAATGCCCACCCCCTAGCAGTCCGGAGATTGCAGCTTCCCTGGAGTATGA CTATGAGACCATCCGTAACATCGACTGCTATGGCACAGACTTCTGCGTGCGTGTGCGGGATGGCATGCGA TACTGGAACATGACCGTGCAGTGGTGGCTGGCACAGTACATCTACAAGAGCGCACCTTTCTGCTCCTACG TTTTGAGGAGTGCCTGGACCATGCTGTTGAGTGCCTACTGGCATGGCCTCCACCCTGGTTACTACCTAAG CTTCATGACCATCCCGCTGTGCCTGGCTGCTGAGGGCTATTTGGAGTCAGCCTTGCGGAGACACCTGAGC CCCGGGGGCCAGAAAGCCTGGGACTGGGTCCACTGGTTCCTGAAGATGCGTGCCTACGACTACATGTGCA TGGGCTTTGTGCTCCTTTCCATGGCTGACACACTCCGGTACTGGGCCTCCATCTACTTCTGGGTCCACTT TCTAGCCCTGGCCTGCTTGGGGCTGGGGCTGGTTTTGGGTGGGGGCAGCCCCAGCAAGAGGAAGACACCA TCCCAGGCTACCAGCAGCCAAGCGAAGGAAAAGCTCCGGGAAGAGTGAGCTCTGCTGCATTGGCCTGCCT TCCAGTTCAAGCTTTTTTGGGAATTCCATGAACCAGGCTGTTTGTTTGGGGTTTTTGTTTGTTTGTTTGT TTGTTTCCTTTACCCAGCAAGAATCCCTTGTTTGGCTAAGAGCCTGGAGAGGATTCCCTCTTCCCAAATA ATTCCTCTGCCTTCTATTTAAAGCTAAGGTATCCTTCTCTTGGGCTCTCTCAGCATCTTGACCTTTTCAG ACCTTCCTCTGCTAACATCAGGGTATTACTATCCACTCTTGAACCTATTATCTCTGCAACAATCTTCAGA TGTTCAAAAAGCCACACTTCCCAAAAATGCCCTTGCAGGGACCAGTGGTCATCTGGCATCTTAGACAGAC TCCCAGTGGGTCCCCAGTATGGGGGCAGGAACTTCAGGGCCAGGTTCTGGGAGAGGGGAGGGATAGCCTT CTTGTTTTCCTCTTTGTTTTTATCATCACACCAGTGTTTCAGAGACCATGGTCTTACACATGCTGAAGGA GAAGCTAAAATGTGAGAAGCCCCAGGGGAGCTTGCTCTTACAGCAGCTTCTGCCTGAGCCATTTCTGGGC TCCCCATACAACCTACCACCCAGTGTCATCCTTGGCCTGTGACAGGCCAGAATGTATAAAGCTTTCCCAA TAAAGTGTTACACATGCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5028759 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5028759 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003110 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source