EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31422

Rab39b ( MGI:1915040)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006878_01	euxassay_006878_02	euxassay_006878_03	euxassay_006878_04	euxassay_006878_05

euxassay_006878_06	euxassay_006878_07	euxassay_006878_08	euxassay_006878_09	euxassay_006878_10

euxassay_006878_11	euxassay_006878_12	euxassay_006878_13	euxassay_006878_14	euxassay_006878_15

euxassay_006878_16	euxassay_006878_17	euxassay_006878_18	euxassay_006878_19	euxassay_006878_20

euxassay_006878_21	euxassay_006878_22	euxassay_006878_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 13 14 17 18 19 weak expression: see section 12

facial vii ganglion

strong

regional

strong expression: see section 05 06 07 20 21

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

cervical ganglion

moderate

regional

moderate expression: see section 10 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 17 18

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 09 18

thoracic ganglion

moderate

regional

moderate expression: see section 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7941
Entity Detected:	Rab39b, ( MGI:1915040)
Sequence:	sense strand is shown >T7941 GTGCGTGGTGCTGATGCTGCTGCCATTTCATCACCTTTGCGAGCGCAGCATCCATCCCTTCGTTCCCCCA GCGCCTGGGCCTACCTTGCTTCAGGCTACCAGGCCAGTGATGTTCTCGCGGCCGATCTAGAAACGGGTTG ATCTGTGCTAAGGCCTTGGTGGGGCGCCCCCTCCCGCCTCCCCAGGCCCGGGCCGAAGAGTCCTGCCAGG CCACTGCCATGGAGGCCATCTGGCTGTACCAGTTCCGGCTCATTGTCATCGGCGATTCCACGGTGGGCAA GTCCTGCCTGATCCGCCGCTTCACCGAGGGCCGCTTTGCTCAGGTTTCAGATCCCACCGTGGGGGTAGAT TTTTTCTCCCGTTTGGTGGAGATCGAGCCAGGAAAACGCATCAAGCTCCAGATCTGGGATACAGCGGGTC AAGAGAGGTTCAGATCCATCACTCGCGCCTACTACAGGAATTCAGTAGGTGGTCTTCTCTTATTTGACAT TACCAACCGCAGGTCCTTCCAGAATGTCCATGAGTGGTTAGAAGAGACCAAAGTACACGTTCAGCCCTAC CAAATTGTATTTGTTCTGGTGGGTCACAAGTGTGACCTGGATACACAGAGGCAAGTGACTCGCCACGAGG CCGAGAAACTGGCTGCTGCATACGGCATGAAGTACATTGAGACGTCAGCCCGAGATGCCATTAATGTGGA GAAAGCCTTCACAGACCTGACAAGAGACATATATGAGCTGGTTAAAAGGGGGGAGATTACAATCCAGGAG GGTTGGGAAGGGGTGAAGAGTGGATTTGTACCAAATGTTGTTCATTCTTCAGAAGAGGTTATCAAATCAG AGAGGAGATGTTTGTGCTAGTTAACCCTTCCATTTTCAAAACATACTCTCCCACTTGAACTTAAAAGTTA TCAAAATAAATAGAATCCTTATGTGACTGAAGAGAACTCAACGTTTGGATAATAAATGAGCCTTCTTCCT AGGCCTGGATCTCTGACAGGCTCATGTGTGGGTAGATAGGGAGCCTGGGTGCTGTGACTGAGGGACTAAA TTGCTGACAGGGGCTGGACCAGACCTTGTTTTGTGGGCCAGGCTCAGCAATGGCTGCTCTGTGTGCCCTT TGTGGACCACATATTAAGGGTCTGGGTATTTGAAAGTTAGGTTCCAGGAAAACACCTACAGACAGATAAA CAGTTCCTTGGTATTACAACTTTTCAGACATGGTGACCATAAAGAAACATATAGAAAGAAATCAAGTATC AATTGGGGACAATTAAGATAACTGAAAAAAAAAAGAGTATGATATCAACTTATTCAATGATAATACCGTG CCAGATGTGTGCTGGGTTTTATACAAACACATCAAGCCACTTCTAACAGTTCAACTGTAAAATGAAAAGC CCATATGAATCAACTATTTGGTAAAGCTCTTTCAAGGTCTAGCATTCTAGTAAAACTGCTGTGAATATGA AAAGGGAAGTCATTCACATATTGTTGTTATCACATTTGAGAAAAGTGTACACTGGCATGATAAAGTTGCC AACTATGTGAAAGAAATTCTTCCATGTAAAATGATCATGTAAGGAGAAAAAGGAAACACTGTAACTCAAA GGCCACATTATTTCCCCTCTAATCATACCTGGACTTTCACTGCATCAGGGAACCATAATCCCGAAGTAAG TATACTGCTTGCTTCTTCAGATACCTGACAACGACAACACAGAGATTGTCAACAAACAAAAACCCTAAGT CAAGCCCTTGGTCATTGGTTCTATTCTGAGTATTGGCTCAACACGTAGTCTTGGAATCCTTAAAAATTAT TCATATTAATTTATTTCACTCATTTGATCACCATAATAGGACTGTGAGATTTTTGATCCCATTTTACATA ATAAGAAAGCAAGAACCAAGCCTACCTGATACCTAGCTTTTTTCACTGGATCATTATTCATATGAATAAG TGATTAATTTATTAACCTTTGGAGACATGTGACTATAAATAAATATTCTCTACATGATAGAATATGTTTT CCTTGTTTTACAGCTCAGCTATAATCTTGCAGGAATCTAGAAATGAATAAAAGGTAAAGGCCACTCAGGT ATTATTGACAAGAGGGCATTAAATAAGGGATTAACTGCACAACTGAAAACACATTAAAACTGTATGGGTT ATAAATGTTCTTCTCACAAACACTATGAATATTTTATTAAAAGGAACCAAATACAACAATGGGGAGTGGG TTGGGGATAAAGTACTCACCTCACAATGACCTTGACTACCTGTCTGCTATGTACCTACATTGGGCTCCTG TCTACCCGAGAGAGTCAGCTCTTAATTCTCAGGTGAACAGACAGCTAACCACATCAGCAAATTAGAATGG CTGAGATGAAAAATTCATTATCTTCTTAAAAAGAAGTGATGCTTTAAATTTCTTTTTGGATTGTAGCCAG TTCAGCTAGGCAAAAGCCATACTTTTGACAGTAAGAAAACTATTCTGATTAGAGTCAAGTTTCTCATATA TACATTGTGGTAGAAAGAATGGCAAATTATATTTGCAATATCCTGTTCGAGTTCAGAATGAATATGTGTG AATCTGGGAATTTGTTTCAGCTAAAATAACCCAAATAATAAAAATTATGAAAGGTTGTTTAGCCACCTTT GCTCCAACTTGTATAAACAAGTCATTTTGGACCAATTCACATTATCAATTGCAGATAAATGATCATTCAT TCTATAATCATTTCACGTATTTTAAATTGCCAGATTCTTGGTTGTAGAAGGCAAATACAACCTTTTTTGT GGGTTAACACAAAATTTCAACAATGATACACATGTCTAGATTGTTCTAAAACCCAGGATTATCCAGTGGA TAAATAAGAGTTGATTAGACTCTTGTTGACTCAAGGGGGCAGGGGTAGAAATAAAACAGGTCTACAGTTT TCTTGACGAAAATACATTATATACTTTGCCATTCCTGGCTTTTAAAAGTGGGTTTCAAAGTACCCAGCAT AAATGCAGCAGAGGTGGCTGAACTTGACTTCTTCCATAAGTTTTAAAAAAATCTCAAAAATCCTTTTAGT AGAATTCAGTGCTGTTGTCACTGCAATAAGTGGGTTTTAGACAATAAATGTGAAAAAATCAGGTGTTCAT AAAATACTAAGGATAATGCATTAGAAGCTATTTTACTACATAGGTAAAGAAAAGATTAACTGTAACAACG ATCACAACTGCATGTTGGATTAGATTGTCTTATATTCCAGAACAAAATGTACTGTATACTTTGCCTCAAT TTGTTGTACACTGTAATGAAATGTGAAAAATTCTTTATTTAGCTGTATGTGAATGAAAATATGCTTGTAT TTAGTAAAATGGTTGATCATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6517891 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6517891 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006878 same experiment
	EMAGE:29384 same embryo
	EMAGE:31394 same embryo
	EMAGE:31453 same embryo
	EMAGE:31445 same embryo
	EMAGE:31434 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source