EMAGE mouse gene expression spatial database

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EMAGE:31434

Nqo2 NAD(P)H dehydrogenase, quinone 2 ( MGI:104513)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

adenohypophysis

strong

regional

strong expression: see section 12 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8212
Entity Detected:	Nqo2, NAD(P)H dehydrogenase, quinone 2 ( MGI:104513)
Sequence:	sense strand is shown >T8212 TAGACACTGGCTGTGTCACATTGTGCGTGTGTTCCCTCCAGGAGGCAGAGGTCCCTGGCTGCATCACCAG CCACTCACTTCAAACCAGAGCCCAGCTAAATAACCTTTCTTGCTGTTTCTTGAACTCTCCAGAGTGCTAC AATAACCAAGGAGAGTAACAGTACCTGAAACTTTTGGCTGTTATACCAAATCAGAGAATCTATCTCCTCC AACATGGCAGGTAAGAAAGTGCTCATCGTCTATGCACACCAAGAACCCAAGTCCTTCAATGGGTCCCTGA AGAAAGTGGCTGTTGAAGAACTGAGCAAGCAGGGATGCACAGTCACTGTGTCTGATTTATATAGCATGAA CTTTGAGCCAAGGGCCACAAGAAATGATATCACTGGTGCCCCCTCTAATCCTGACGTCTTCAGTTATGGG ATAGAAACCCATGAAGCCTACAAGAAGAAAGCTCTGACCAGTGATATATTTGAAGAACAGAGAAAGGTGC AAGAAGCTGATCTTGTGATATTTCAGTTTCCACTATACTGGTTCAGCGTTCCAGCAATCCTAAAAGGTTG GATGGATAGGGTGCTGTGCCGAGGGTTTGCCTTTGATATCCCAGGCTTTTATGACTCTGGTTTTCTCAAG GGTAAATTAGCTCTCCTTTCCTTAACCACGGGAGGTACAGCGGAGATGTACACAAAAGATGGGGTCAGTG GAGATTTCCGGTACTTCCTGTGGCCACTTCAGCATGGTACACTGCACTTCTGTGGATTTAAAGTCCTTGC CCCCCAGATCAGTTTTGGTCTTGATGTTTCATCAGAAGAAGAAAGGAAAGTGATGCTGGCATCATGGGCC CAGCGGCTGAAGAGCATCTGGAAGGAAGAACCCATCCACTGCACACCCCCTTGGTACTTCCAAGAGTAAC ATTTTGTGCTCTGAGTACAGCTGACAAGCAACACAGTGAGAGACCTACAGCATGTGCAAAGAGAAGGTGG TGTTGTATCCTGAGATGTATTTAACAGTGCCCACCAATGAGTGTCTTCAGTTTAATACAACTAGTTCAGA TATTTCAAAAGTCAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1225122 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1225122 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006873 same experiment
	EMAGE:29384 same embryo
	EMAGE:31394 same embryo
	EMAGE:31422 same embryo
	EMAGE:31453 same embryo
	EMAGE:31445 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source