EMAGE mouse gene expression spatial database

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EMAGE:31435

Os9 amplified in osteosarcoma ( MGI:1924301)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003123_01	euxassay_003123_02	euxassay_003123_03	euxassay_003123_04	euxassay_003123_05

euxassay_003123_06	euxassay_003123_07	euxassay_003123_08	euxassay_003123_09	euxassay_003123_10

euxassay_003123_11	euxassay_003123_12	euxassay_003123_13	euxassay_003123_14	euxassay_003123_15

euxassay_003123_16	euxassay_003123_17	euxassay_003123_18	euxassay_003123_19	euxassay_003123_20

euxassay_003123_21	euxassay_003123_22	euxassay_003123_23	euxassay_003123_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 17 18 19 21

cervico-thoracic ganglion

weak

regional

weak expression: see section 10 16

clavicle

strong

regional

strong expression: see section 10

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 18 19

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 16 17 18 19

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 20 21

cornea stroma

strong

regional

strong expression: see section 01 02 03 23 24

meckel's cartilage

weak

regional

weak expression: see section 03 04 05 06 07 08 09 18 19 20 21 22

lower jaw molar

weak

regional

weak expression: see section 05 06 07 08 09 18 19 20 21

vagus x ganglion

weak

regional

weak expression: see section 08 17

upper jaw molar

weak

regional

weak expression: see section 05 06 07 08 09 18 19 20 21

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22

cervical ganglion

weak

regional

weak expression: see section 09 16

liver lobe

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 17 18 19 20 21 22 23 24

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4187
Entity Detected:	Os9, amplified in osteosarcoma ( MGI:1924301)
Sequence:	sense strand is shown >T4187 GCGGAAGATGGCGGCGGAGGTGCTGCTGTCCAGTCTGTTGGGATTGCTATTTCTAGGGCTCCTGTTACCT GCGCGTCTGACGGGCGGTGTCGGGAGCCTGAATTTGGAGGAGCTGAGTGAGATGCGTTATGGCATCCAGA TCTTGCCGTTGCCTGTCATGGGAGGGCAGAGCCAAGCTTCAGACGTGGTGGTTGTGTCTTCAAAGTACAA ACAGCGCTATGAGTGCCGCCTACCAGCTGGAGCTATTCACTTCCAACGTGAAAGAGAGGAGGAGACGCCT GCTTACCAGGGGCCCGGGATCCCTGAATTGTTGAGCCCAATGAGAGATGCCCCTTGTTTGCTGAAGACCA AGGACTGGTGGACATATGAATTCTGTTATGGACGTCATATCCAGCAGTACCATATGGAAGACTCGGAGAT CAAAGGTGACGTCCTCTATCTTGGCCACTACCAGTCCTCCTTCAACTGGGACGACGAAACAGCCAAGGCT TCCAAGCAGCATCGGCTGAAACGCTACCACAGCCAGACCTACGGCAACGGGTCCAAATGTGATCTCAACG GGAAGCCCCGAGAAGCTGAAGTTCGGTTCCTGTGTGACGAGGGTGCGGGCATATCTGGGGACTACATTGA CCGAGTAGATGAACCCGTCTCCTGCTCCTACGTACTGACCATTCGCACGTCAAGGCTCTGCCCGCATCCT CTCCTCCGGCCACCAGCCAGCGCTGCCCCACAGGCCATTCTTTGTCACCCAGCCCTGCAGCCTGATGAGT ACATGGCCTACCTCCAGAGGCAAGCTGAGTCAAAGCAGCATGAAGAGAAAACCACAGAGGAAGTCCAGGA CACAGACCGCCAAGTGTGGAGTGGGAGCAAGGCTGCCGGAGCACCCCCAAAGAAAGAAGATGTCAGCCCA GCCAAGGAAGAGAAGGAATCAGAGCTCTGGAAGCTTCAGGGGCCAGAGGAGCAGGCAGCAGCAAGAGAGG AGGCGCAGGCAGGGGAACAGGACCTGAACCACGAGGCCGCAGCAGATCCAGCTCCAAGCCCTCCCAATGA TTTTCAGAATAACGTGCAGGTGAAACTCATCCGGAGTCCCGCAGACTTGATTCGACTGATTGAGGAGCTG AAAGCTGCAGAAAAGGGGAAGCCAAGCGTAAGGCGGGAGCAGCCTGGAGACGATACCACGGAGGCCCCCC AGAGGGAAGCAGAGGGAACGAAGGCGAAGGGAAAGGGTGGTGAGCCCCCGGGTCTTATGGAAGAGGAGGA TGGTGACGACGAAGAGGAGGAGGAGGAGGAGGAAGAGGACGAGGAGGAGCAGCAGCTCCTGGGAGAGTTC GAGAAGGAGCTGGAGGGGATGTTGCTGCCCTCAGACCGCGAGCGCCTCCGCTCTGAGGTTAAGGCTGGCA TGGAGCGGGAGCTGGAGAACATCATCCAGGAGACAGAGAAGGAACTGGACCCAGAAGGGCTGAGGAAGGA GTCCGAGCGGGAGCAGGCAATATTGGCTCTAACATCCACTCTGGACAAACTCATCAAGAGGCTGCAGGAG AACCAGAGTCCAGAGCTTGTGCAAAAATACAAAAAAAGGAGAGTTGTCCCCCAAAAGCCTCCCCCATCAC CACACCCTACAGGCAAGATTGAGATCAAGATTGTGCGACCCGGGGCTGAAGGTAAGGAAGAGGACACACG CTGGCTGACTGATGAGGATACAAGAAACCTTAAGGAGATTTTCTTCAATATCTTGGTACAGGGAGCCGAA GAGGCCAATAAAGAACGCCAGCGACAGAGTGAGCTGGAGAGCAACTACCGCCGGGTGTGGGGCTCTCCCG GTGGTGAAGACACGGGGGACCTGGATGAATTTGACTTCTGAGACCACCCTGCCAGCCCTCTAAGGATTCC TGCATCTTCCTGACCGGCCGGCTCTGCCTCCTTACACCCCATTCTGGGGCCTTGGAGGCAAAAGGGCAGA TTGGAGCCCAGCACTGGACTTGAGGGGCCCAGCCCCAAGAGTGTATGAGGGTGTGTGGGGACCCATGAGG GTGAGGAGCTGTTGCAGCCAGGCCTATGGGGTCCACTCCCCAAACACACCCTCCTTTGCCCCTTTAGCTT TTTCCTGTCCCCCAACTTCTTAGCACTTAACCCCACTCTACCCAGAGACTTGATGAGACCTTGTGAGTGG TGGTGGCAGGAACAGCAGGGCTGTGCCGGTCCCCGACCCCTCCTCTCCCTATCCTACCTCCATTCCCTGT TGCTGTTTGGGAGCTGTTGCCCCTCCCTTGGAACGCCTACTTCCTGTCTCTGCTGTCTCCCGGTACCTGT GCTTCTGAGCCCAGACTATCTCTTGTCACTGAGGGGATGGGAGACCTTTCATATCACCTCACAAACAATC CCAGCAAAAAGGGTTGGAGATGGAGCACCCCCTTGTTCTTCAGCAATCAGGTTTCTAAATAAAAAACTGG ACCGTCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4487829 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4487829 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003123 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source