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EMAGE:31441

Ces2 ( MGI:2385905)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31441 EMAGE:31441 EMAGE:31441 EMAGE:31441 EMAGE:31441
euxassay_002007_01 euxassay_002007_02 euxassay_002007_03 euxassay_002007_04 euxassay_002007_05
EMAGE:31441 EMAGE:31441 EMAGE:31441 EMAGE:31441 EMAGE:31441
euxassay_002007_06 euxassay_002007_07 euxassay_002007_08 euxassay_002007_09 euxassay_002007_10
EMAGE:31441 EMAGE:31441 EMAGE:31441 EMAGE:31441 EMAGE:31441
euxassay_002007_11 euxassay_002007_12 euxassay_002007_13 euxassay_002007_14 euxassay_002007_15
EMAGE:31441 EMAGE:31441 EMAGE:31441 EMAGE:31441 EMAGE:31441
euxassay_002007_16 euxassay_002007_17 euxassay_002007_18 euxassay_002007_19 euxassay_002007_20
EMAGE:31441 EMAGE:31441 EMAGE:31441
euxassay_002007_21 euxassay_002007_22 euxassay_002007_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4578
Entity Detected:Ces2, ( MGI:2385905)
Sequence:sense strand is shown

>T4578
GAAACTTGGTCATCTTAGCAGCACAGACTTAGGACTGAGAGCTGATCATGACACGGAACCAACTACATAA
CTGGCTGAATGCTGGGTTCTTCGGGCTGCTGCTTCTTCTTATCCATGTGCAGGGTCAGGACTCACCAGAG
GCCAACCCCATCAGAAACACACATACAGGACAGATCCAAGGCAGCCTTATCCACGTGAAGGACACTAAAG
CTGGTGTCCACACCTTCCTGGGAATCCCCTTTGCCAAACCTCCTGTAGGACCACTGCGATTTGCACCTCC
TGAGGCCCCTGAACCATGGAGTGGTGTGAGAGATGGAACTGCACATCCGGCCATGTGTCTGCAAAATCTT
GACATGCTGAATGAAGCAGGCCTGCCAGATATGAAAATGATGCTGTCTTCCTTCCCTATGTCTGAGGACT
GCCTGTATCTCAACATCTACACCCCAGCCCATGCCCATGAGGGCTCTAACCTGCCTGTGATGGTGTGGAT
CCATGGAGGTGCACTGGTTATAGGAATGGCTTCCATGTTTGATGGATCTCTATTGACAGTCAATGAGGAC
TTGGTGGTTGTTACTATCCAGTATCGTCTGGGTGTCCTGGGCTTTTTCAGCACTGGAGACCAGCATGCCA
GAGGCAACTGGGGATACCTGGACCAAGCAGCTGCCCTACGCTGGGTCCAGCAGAACATCGCTCACTTTGG
AGGCAACCCTGACCGGGTCACTATTTTTGGCGAGTCTGCAGGTGGCACAAGTGTGTCTTCACATGTTGTG
TCCCCCATGTCCCAAGGGCTCTTCCATGGTGCCATCATGGAGAGTGGGGTGGCCCTGCTTCCTGACCTTA
TCTCTGAAACCTCTGAGATGGTTTCCACTACGGTGGCCAAGCTTTCTGGATGTGAGGCTATGGATTCCCA
GGCCCTGGTGCGCTGTCTGAGAGGCAAAAGTGAAGCAGAGATTCTGGCTATTAACAAGGTCTTCAAGATG
ATCCCTGCTGTGGTGGATGGGGAGTTCTTCCCCAGGCATCCCAAAGAGTTGTTGGCATCTGAGGATTTTC
ACCCTGTCCCCAGCATCATTGGTGTCAACAATGATGAGTTTGGCTGGTCCATTCCTGTGGTCATGGGCTC
TGCTCAGATGATAAAAGGAATAACCAGAGAGAACCTGCAGGCTGTTCTGAAGGATACAGCAGTACAAATG
ATGCTGCCTCCTGAGTGTAGTGACCTGCTAATGGAAGAGTACATGGGGGACACTGAGGATGCCCAGACCC
TCCAAATACAGTTCACAGAGATGATGGGAGATTTCATGTTTGTGATCCCTGCACTCCAAGTAGCACATTT
TCAACGCTCCCATGCCCCTGTCTACTTCTATGAGTTCCAACATCCACCCAGCTACTTCAAGGATGTCAGA
CCACCCCACGTGAAGGCTGACCATGCTGATGAGATTCCTTTTGTCTTTGCGTCCTTCTTCTGGGGCATGA
AACTTGACTTCACTGAGGAGGAGGAGCTGCTGAGCAGGAGGATGATGAAGTACTGGGCCAACTTTGCACG
ACATGGGAACCCCAACAGTGAGGGTCTACCCTACTGGCCTGTGATGGACCATGATGAGCAGTACCTGCAA
CTGGACATCCAGCCTGCTGTGGGTCGAGCCCTGAAGGCTGGAAGGCTGCAGTTCTGGACCAAGACTCTGC
CCCAGAAGATCCAGGAGCTAAAGGCTTCTCAGGACAAGCACAGGGAGCTTTAGTACCCTGTATCAAAAAA
AATGTGTTTGGGGTTTAAAGTCAATAGCGCTACAAATTCCATTTATTTATTTTTATTTGTTCCAACACTT
ACATAAGCATCTGTCACCTCAGTCTTTGAGCTTTCATGTATGAAAAATACTCTGTATTACTTATCATTCT
GGCCAAACTTTCATTAAATTCAATAAATTCTCAAATAACACTGTGGAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4984806 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4984806 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002007 same experiment
 EMAGE:31725 same embryo
 EMAGE:29890 same embryo
 EMAGE:31476 same embryo
 EMAGE:31865 same embryo
 EMAGE:31204 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS