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EMAGE:31444

Emd emerin ( MGI:108117)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31444 EMAGE:31444 EMAGE:31444 EMAGE:31444 EMAGE:31444
euxassay_003336_01 euxassay_003336_02 euxassay_003336_03 euxassay_003336_04 euxassay_003336_05
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euxassay_003336_06 euxassay_003336_07 euxassay_003336_08 euxassay_003336_09 euxassay_003336_10
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euxassay_003336_11 euxassay_003336_12 euxassay_003336_13 euxassay_003336_14 euxassay_003336_15
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euxassay_003336_16 euxassay_003336_17 euxassay_003336_18 euxassay_003336_19 euxassay_003336_20
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euxassay_003336_21 euxassay_003336_22 euxassay_003336_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
vibrissa
moderate moderate
regionalmoderate expression: see section 20 weak expression: see section 05 06 21
epidermis
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 weak expression: see section 01 02
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6367
Entity Detected:Emd, emerin ( MGI:108117)
Sequence:sense strand is shown

>T6367
ACAGGCATGCATGACAACTACCACTGCCACCACCAAGTTTTCCTCTAATGAAGTGAGGTCCTCCCACCAG
ACCCCGGTACCCTAAGGGTCAGAGGCAATAGGGCTAGGAGGCTCCAGAGGAAGGTACAGGTAGGCCTACT
GGGCTGTAGAAGGCCAACATTTGCTGCTTCCTTGGCAGGATGAGGTGGGAGACAAGCATTGCCTTAGGCT
AGACCTTGCACCCAACTAGAACACACAAAAAAGCCCCTACCCTCCCCACCCCCAGCAAACACCGATAAGG
CAATCAGCCAAAACTCCTGGTAAGAGCTGCTCTAAAACCAATACCCTTAGGGATCCATCCAGAAGGGGTT
GCCTTCTTCAGCTTGTATGGAATAGTAAACAAAGAGCAGAAAGGCAGCAAAGACCAGGAAGAGTAACAGC
TGGCCCCAGAGTGGGACCTGTCGATCCTGGCCCAGAGCAGCAGCAGGGGCCTGCTTCTCTGGCCGAATGG
CACGTCGGGTAAGCCATGAAGAGGGAGAAGATGAAGAGGAGGACACAGAAGAGGTGGAGGATGTAGGATA
ATATGATAAG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers.. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_003336 same experiment
 EMAGE:29385 same embryo
 EMAGE:29398 same embryo
 EMAGE:31485 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS