EMAGE mouse gene expression spatial database

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EMAGE:31447

Zfp354c zinc finger protein 354C ( MGI:1353621)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005516_01	euxassay_005516_02	euxassay_005516_03	euxassay_005516_04	euxassay_005516_05

euxassay_005516_06	euxassay_005516_07	euxassay_005516_08	euxassay_005516_09	euxassay_005516_10

euxassay_005516_11	euxassay_005516_12	euxassay_005516_13	euxassay_005516_14	euxassay_005516_15

euxassay_005516_16	euxassay_005516_17	euxassay_005516_18	euxassay_005516_19	euxassay_005516_20

euxassay_005516_21	euxassay_005516_22	euxassay_005516_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9586
Entity Detected:	Zfp354c, zinc finger protein 354C ( MGI:1353621)
Sequence:	sense strand is shown >T9586 GTTCTCTGGGGCTTCTCCCGGTGGCGGGGCTGCTCGCGGCGTTTTGGGGGGCTCCTGGTGGAGGTGATCC TGACTGTGCTAGGAGAGAGCGTCTGGGGCCGACATCCTCGAGACTGCTCGTGCTGAGAGTGGTGGGAGCG AGCGCCGGCGTCCGCATATTTGGTCAGCACTCGGCTCTCCTTTGGCAGAAAAACTAAGAAGGGAAATGGC TGTGGATTTGCTGGCTGCCCGAGGCACAGAGCCTGTGACCTTCAGGGATGTGGCCGTGTCCTTCAGCCAG GATGAGTGGTTGCACCTGGACCCTGCTCAGAGAAGCCTGTACCGAGAGGTGATGCTGGAGAACTACAGCA ACCTGGCCTCGCTGGGGTTTCAGGCTTCAATACCTCCTGTGATTGGCAAGCTGCAAAAAGGGCAAGATCC CTGCATGGAAAGAGAAGCCCCTGAAGACACCTGCCTAGATTTCGAGATTTGGCCAGAGATAGAAGCATTG CCACCCAAACAAGATGTTCTTACTAAAGAGACATCACATGGGCTGATAAAGAATGGATCCACTAAGTGTG TTTACTGGAAAATCAGCTTTGGAGAGCTTGTGAAAACTGAGTGCCGAGATATAGCACAGGAGCAAGAAAA GAAAGTGCATGGGCCAGGGGCAGAGTCACCCAAGGAAACCACCAGTGAAGACGGAACTCCCACGGGCTTC GAGCCGGAGAAACCCTTGTTCATTAGTAAAGCACTTGTCTCACAAGAGGGTGATCCTACAGAAAGTGTTC CTGCTACGTATCACACTTCAGAGAAAGATTTACCACAGGATTTTGATCTAATGAGAAGCTTTCAAATGTA CCCAGGACAAAAACCTCATGTCTGCAGTGAATGTGGCAAGGGTTTCACCCAAAGCCTTCATCTTCTAGAG CACAAGAGGCTTCACACTGGGGAGAAGCCGTACAAATGCAGCGAGTGTGGGAAGAGCTTCAGCCATAGAT CCTCCCTCCTGGCTCACCAGAGAACCCACACCGGAGAGAAGCCGTACAAGTGCAGCGAGTGTGAGAAAGC CTTCGGCAGCAGCTCCACTCTCATCAAACACCTGAGGGTGCACACCGGAGAGAAACCTTACCGCTGCCGG CAGTGTGGGAAGGCCTTCAGCCAGTGTTCCACCCTCACTGTACACCAGAGGATTCACACTGGGGAAAAGC TCTATAAATGTGCCGAGTGTGACAAGGCCTTTAACTGCAGAGCAAAGCTCCACAGACATCAGAGAATCCA CACAGGGGAGAAGCCATATAAATGTGCTGAGTGTGGGAAGGGCTACAGTCAGTTTCCATCCCTAGCCGAA CACCAGAGACTCCATACTGGCGAGCAGCTTTGCCAGTGTTTGCAATGTGGGAGAACCTTTACACGTGTGT CTACCCTTATCGAACACCAGAGAATTCATACTGGACAGAAACCATATCAATGCAACGAATGTGGGAAGAC TTTCAACCAGTATTCCTCCTTCAATGAACATCGGAAGATTCATACCGGGGAGAAACTTTACACATGTGAA GAATGTGGGAAAGCCTTTGGCTGTAAATCAAACCTTTATCGGCATCAGAGGATCCATACTGGAGAGAAAC CCTATCAGTGTAACCAGTGTGGAAAGGCCTTCAGCCAGTATTCATTCCTAACTGAACACGAGCGAATCCA TACTGGAGAGAAGCTCTACAAGTGTATGGAGTGTGGGAAAGCCTATAGTTACAGGTCAAACCTTTGTAGA CACAAAAAAGTCCACCTTAAGGAAAGGCTGTATAAATGGAAAGAATATGGGACACCCTTTATCTATGGCT CATCCCTTACTCCATATCAGAAATTTCTGAAAGGAGACAAGCCTGAGAACTTTAATTCATCGCTGTGATC CAAGACAGCAACCAGAAGAGTAGTGAGTAGAATGCAAATCAAAATAGAGTCATCTTTTTTACTTGTACAG CAAATAGTTACCACTAGTCATGGACAGTGGATGCACAAAGAATGCTGACATCAGCAATTTAAGGACTATT ACAAACAATGTTAAAAATTCGTAGAAAAAGTTTGAAAGGCCTCATTTAAAAAACAAAGCGCATGAGTAAC TGTTGCATATAAGCATTGTTTCATGAGAGGGCCATCTCTTTCTAAGAGCTTCTGTGTTATGATTTTTCTT TTTAAGATAGTCTTTGATGTAGTTAATAATGTGAAGAATCGTGGGGACTCAGTCCCTAAAATGGTGAGCT GTTATAGGGAGAAGTCCTTTTCATAAGGAGCCACTACAGAGGAAAAGAATACTTTGACTTGTTGTGTATC AACAATAAAGCTCTTTATAAATTGCCCCTTATACAACATACCTGTGTGTATGTATGCATTTATATTCAGA TCTACACATAAAGGACTTTTATGTATATATGGCCATGTCACCTAACAATGGGGATACAGTTAGAAAAGGC TTCATTAGGCACTTTCATGTTTATAGGAACATCATTGCATTTGCATAGCCCTAGATAGTCTGGGTCAGCC AATCAATGTGATCTCTTGAGGTGATCAACATACCATAATTTAAAAAAAAAGTTTTTTGAGGTTATAATTA CAACATTTCTCCCTTCCCTTTCTCCAAACCCTCCTATACACTCCTCCCCACTCTCCTTCAAATCCATGGC CTCTTTTCTTACTAATTGTTATGCATGCATATATCCATACTTTTTTATAGTAAGGGTTTTTATTGATTGG CTTGTTTATTTTAGATAGAGTTTCACTATGTAGCCTAAGATGGCCTTGAACTCATTATGTAGAGCAGGCT GGTTTTGAACTTGCAGTCTTCCTGCATCAGCCTCCCACGTACTGGGATCAGAGGCATGAGCCATCATGCT TGGTGTAAATTTTATTAGTAGAATGTGTATACTCTAAGGATAAAAGTATAGTATAGCAAATGAGTAAGCT GGTAATGTAGTTTGTCACCATCACCATGTGTCATGTACTGTATATAATTGCGTGTGCCGTGATGTCTGGC TGCCTGCACAGAGGGTTTGTTTGCACAAATGGGCAATGCATTGTGCCTAAAGTGCTATAATAGTTAAAGA GTCACGACAGCTCTTATGTAATCGGACAAGTAGGAATTTTCCAGACCAGCTGTAGTCTGATGAGACCACT CTAACATATGTTGTCATTGGCTAAAACATCCTCATAGGCAAATGGCTGTATGCACACACTCACATATATG TACATACATGTGTGTGCCAAAGTGGATACATAACACATGCAAAGAGTTTAGGACTTTTTTTTCTCTCCAT AGCCCTTAATGATTAAAAAAGACTCAGTTTACTAATTTAGAATTTACTGTTTCAAATTAAAAATTTCCCT CAGGGGCGACTTCTTTCTGACTCCCCTATATCTGTAGTGGGCTCTGGTGTAGGATCTAGCTGAATAGAGT CTTACAGTACTATAGAAGTTGGTCTGAGCAGACAGGTGGGCAGTCTGTCATCCAAACAATATATTACGTA TTGTAGAAGGCCATTGTTAACAAGAGTCTCCTGCTATGAACCTGTTTGCATTTTAGAATCAGAGAAACTT TTAATTTATAACCTTTTAAGAATCGAGGTCTTCAGCTCTGATAATCTATGATAGTTATAGGTTCATTTCT ATCCTCAAGGCTCAAGAACACTAATTGTTTTGTAACCTGTTCAAATATTTTTGCTAGTAATGTAATCAGT TATCTTTAGTTTCTCCTAGCTTTGGTCTTAGTAGTGGAGTTGAGAACATGCCCCCTTCCTTCTATCTCTG ATTCTATACCTGCTTCTATAACTACTTAAATTCTCAAAGCCAGCGACAGCCCAGATGGAGGTATCGCTTT GGTATGTTTCCGTGTGTGGTTGCCTTTTTATTACACAGCTTTTAATATTTTTTTCCTTGTAGTGCTGACG ACTGAATCCAGGGCCTTGTGCATGCTCAGGAGGTGCTCTCCCACTGAGCTGTGTCTGCAGCCCTTAGGAT TTCAGTATAAAGTTGCAGATGAATATTTTATATTAGACAATCTACTCATTACCTTAATATTTTATAACTA CCCTAGACCTAGTTCCTTTTATCTTTGACTTATCCCACTGGTTTGTCACTTTAGAATTACTCCAGGAGCA TTAATCAGACCCTTCTGTCTGAGCAAAATATTAGAATTAAGCAGGCATTCAGCACTTAATTGACTTCTTT TGTCTCATCAGTAGTTGTATGTGTTTAAATGTATTTGCAGGAAAAATAAGACTTGAAGGGAGCCTCTGAG TGTCTTCTTGTCCCTAGTGAGCATGCTCAGTGCCCAGTGTTTGATTCACAAATGCCATTTGCCTCCAGAG GGACTCAGGCCTCCTGGAAGAAGTGAACAGTGGCACGTTTGCAGTGAGAGGGGAAGGCAAGTGCACAGAG AGGATAGTTCTGTGTTAGGACATCATTTTAGGGTTATTGATATGAGTAGACACAGGGACTAACTTGAAGG TGCATCCTTTGATCAAACTTTAAGACACTTCAAACAAAATACTCATGACAGGGTTGGATATAATATTTTC TAAAGGTTATGGGGGGAAAAGCTTTTTATAACATTTGAAGAAAAGCATCCTTTAAATATATACAAAGCAA TAACAAAAGCCTTTTAGCAACTGTCATTGTCATTAAATCTAGCAAAAAGTTGATTGATGGTAAAGCCTAT TTCATGAAAGACAATTAAGGAACATTTCCTCATCCCGAGAGTACAGCTCTGAGTCGCAAGAGACACATCT GCTTTCATCCTGAGATCTGGCAGGCAGAAGTCCCCTCCTGAGCAGCAGCCACTGTAGCTTCTCATGAGGC CGCAGAAGCACATTTTACTATTTATGACATACTGCCAAAACCAGTTAGCGAGCATCTATGGAAATCTCTT CTCTAGACATACCTTTCTGCTTACAAAGAATTGAGAGTAACATGTTAGATTATATGGTAATCTAGAGTTT GAGACATTGGAGATGATAATTAAGTTGTACTTTTCAAATAGCCAATATGTTGGAACAAAAAGATGCCTGA GACAGCCACATGCAAGGTGTGATCTTTTATTGAGAAATAAAGTCAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30654724 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30654724 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005516 same experiment
	EMAGE:30483 same embryo
	EMAGE:30481 same embryo
	EMAGE:30476 same embryo
	EMAGE:30353 same embryo
	EMAGE:29534 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source