EMAGE mouse gene expression spatial database

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EMAGE:31449

Abcd4 ( MGI:1349217)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_012296_11	euxassay_012296_12	euxassay_012296_13	euxassay_012296_14	euxassay_012296_15

euxassay_012296_16	euxassay_012296_17	euxassay_012296_18	euxassay_012296_19	euxassay_012296_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 weak expression: see section 03 04 05 06

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30457
Entity Detected:	Abcd4, ( MGI:1349217)
Sequence:	sense strand is shown >T30457 AGGCACTGACCTTGCTGGCCGTGACACTTATTGTCCTCAACTCCACCCTGAAGAGCTTTGACCAGTTCAC CTGCAACCTGCTGTACGTGAGCTGGAGGAAGGACCTCACGGAGCACCTGCACCACCTGTACTTCCGGGCC CGCGTGTACTACACCCTCAACGTGCTGCGGGACGACATTGATAATCCGGACCAGCGAATCAGCCAGGATG TGGAGCGATTCTGCCGCCAGCTCAGCAGCGTGACCAGCAAGCTGATCATTTCCCCCTTCACTCTTACCTA CTACACTTACCAGTGCTTCCAAAGCACAGGCTGGCTCGGGCCTGTGAGCATCTTTGGATATTTCACCGTG GGGACCATGGTGAACAAAACTTTGATGGGGCCCATCGTGACGAAGCTGGTGCAGCAGGAGAAGCTGGAGG GGGATTTTAGGTTCAAGCACATGCAGATTCGAGTGAATGCAGAGCCTGCGGCTTTCTATAGAGCTGGGCT CGTCGAGCACATGAGGACAGACCGCAGGCTGCAGAGACTCCTTCAGACCCAGAGAGAGCTGATGTCCAGG GAGCTCTGGCTGTACATTGGTATCAACACCTTTGACTATCTGGGCAGCATCCTGAGTTATGTCGTCATCG CTATCCCCATTTTCAGTGGGGTCTATGGAGATTTGAGCCCCACAGAGCTTAGCACCCTGGTCAGCAAGAA TGCCTTTGTGTGCATCTACCTCATCAGCTGCTTCACCCAGCTCATTGACCTGTCCACCACACTCTCGGAT GTTGCCGGTTACACACACAGGATTGGAGAGCTTCAGGAGGCCCTGCTGGACATGTCCCGAAAATCACAAG ACTGTGAAGCCCTAGGCGAGAGTGAGTGGGACTTGGACAAAACCCCAGGGTGTCCAACAACAGAGCCATC AGACACAGCTTTTCTGCTCGATCGGGTTTCCATATTGGCCCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:30012556), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58797. Forward Primer - name:058797_F_IRAV106_c10_Abcd4, sequence:AGGCACTGACCTTGCTGG; Reverse Primer - name:058797_R_SP6_IRAV106_c10_Abcd4, sequence:GGGGGCCAATATGGAAA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_012296 same experiment
	EMAGE:32066 same embryo
	EMAGE:29298 same embryo
	EMAGE:29287 same embryo
	EMAGE:30991 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source