EMAGE mouse gene expression spatial database

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EMAGE:31454

Ctsf ( MGI:1861434)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000062_01	euxassay_000062_02	euxassay_000062_03	euxassay_000062_04	euxassay_000062_05

euxassay_000062_06	euxassay_000062_07	euxassay_000062_08	euxassay_000062_09	euxassay_000062_10

euxassay_000062_11	euxassay_000062_12	euxassay_000062_13	euxassay_000062_14	euxassay_000062_15

euxassay_000062_16	euxassay_000062_17	euxassay_000062_18	euxassay_000062_19	euxassay_000062_20

euxassay_000062_21	euxassay_000062_22	euxassay_000062_23	euxassay_000062_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

moderate

homogeneous

moderate expression: see section 10 11 12 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2157
Entity Detected:	Ctsf, ( MGI:1861434)
Sequence:	sense strand is shown >T2157 CTCGAGCCAGAATTCGGACGAGGGTGANTCCGGGCGAGCAGCTTCGACCTCGCTATGGCGCCCCTACTGC AGCTTCTGTGGCTGCTGACACTGCTCTCGACTGTGGCCCTGAGCCCCGTCCCCGCCAAGCCCTGGGCAGA TGACGAGCAGGCCTGGAACTTGTCGTCCCAGGAGCTGCTGGCCCCCGCCCGCTTCGCGCTGGACATGTAC AATTACGGCCGTGCTGCGGGGACAAGGGCCGTGCTAGGAGCGGTGCGCGGACGCGTCCGCCGGGCCGGCC AGGGCTCTCTGTTCTCCCTGGAAGCCACACTAGAGGAGCCACCTTGCAATGATCCCCTGGTGTGCCCACT GCCTGAGACGAAGAAAACAGTGCTCTGCAGTTTTGAAGTCCTGGAAGAGCTAAAAGAACACTTGCTGCTG AGGAGGGACTGTAGCCCAGTGAATGCCAAGGTCACAGAGTTTAGAAATGCGACTTTCAGCTCATTCCTTC CATTGTTGGACAAGGATCCCCTGCCCCAGGACTTCTCTGTG
Notes:	The probe template was PCR amplified from IMAGE:875900 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:875900 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000062 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source