EMAGE mouse gene expression spatial database

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EMAGE:31464

Nop14 NOP14 nucleolar protein homolog (yeast) ( MGI:1922666)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003109_01	euxassay_003109_02	euxassay_003109_03	euxassay_003109_04	euxassay_003109_05

euxassay_003109_06	euxassay_003109_07	euxassay_003109_08	euxassay_003109_09	euxassay_003109_10

euxassay_003109_11	euxassay_003109_12	euxassay_003109_13	euxassay_003109_14	euxassay_003109_15

euxassay_003109_16	euxassay_003109_17	euxassay_003109_18	euxassay_003109_19	euxassay_003109_20

euxassay_003109_21	euxassay_003109_22	euxassay_003109_23	euxassay_003109_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

strong

regional

strong expression: see section 14 17 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 09 20

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 11 17

spinal cord

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19

bladder

strong

regional

strong expression: see section 15 16

esophagus

moderate

regional

moderate expression: see section 14 15

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 07 22 23

midbrain

moderate

homogeneous

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 09 18

thymus primordium

weak

regional

weak expression: see section 12 13 14 15 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 09 10 20 21 22 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19 20 21 weak expression: see section 10

hindgut

strong

regional

strong expression: see section 13 14 15 16 17 18

cervical ganglion

moderate

regional

moderate expression: see section 10 17

naris

moderate

regional

moderate expression: see section 14 15 17 18 19 20

hindbrain

moderate

homogeneous

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

stomach

strong

regional

strong expression: see section 07 08 09 10 11 moderate expression: see section 04 05 06

forebrain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

urethra of male

moderate

regional

moderate expression: see section 15 16 17

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07

midgut

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19 20

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 17 18 19 20

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 10 11 18 19 20 21

lower jaw incisor

weak

regional

weak expression: see section 12 13 14 17 18

upper jaw incisor

weak

regional

weak expression: see section 13 14 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4660
Entity Detected:	Nop14, NOP14 nucleolar protein homolog (yeast) ( MGI:1922666)
Sequence:	sense strand is shown >T4660 CCAAAGTGTGGCGGAGTGAATGTCATGGGGAAAGCCAAGCGGACTGGGGCGCGGAGGCAGGTCCACAAAG CGCCCACAGGGGCGTCCGGAGGCCCGGCGAAAACTAACCCAAACCCATTTGAAGTGAAAGTCAACCGGCA GAAGTTCCAGATCCTTGGCAGGAAGACGCGCCACGATGTGGGCCTGCCCGGGGTGTCCCGCGCTCGTGCC ATCAGGAAGAGAACCCAAACTTTACTAAAGGAGTACAAAGAAAGGAACAAGTCCAACGTGTTCGCAGACA AACGCTTTGGGGAGTATAATAGCAACATAAGCCCAGAGGAAAAGATGATGAAGAGATTTGCTCTGGAGCA ACAGCGATATCATGAGAAAAAGAACATCTACAACCTAAATGAAGATGAAGAATTGACTCATTATGGCCAG TCCTTGGCTGACATTGAAAAGCATAATGACATTGTGGACAGTGACAGTGATACTGAAGACCGGGGGGCAT TGTCAGCTGAACTGACTGCTTCCCACTTTGGTGGTGGGGTCCACAAGAACAGCTCTCAGAAGGAGGGCGA GGATGGAGACAAGCCAAAGACACGGAAGGAACTGATTGAGGAGCTCATTGCAAAGTCAAAGCAAGAGAAG AGGGAAAGACAGGCTCAGCGAGAAGATGCCCTTGAGCTCACAGAGAAGCTGGACCAAGACTGGAAAGAAA TACAGATTCTCATGTCACGAAAACCTAAGAAGTCAGAAAACAAAGAGAAAAAGGAAAAACCCCAGCCCGA TGAATATGACATGATGGTTCGTGAGCTTGGCTTTGAAATGAAGGCTCAGCCCTCTAACAGGATGAAAACA GAAGAGGAACTAGCAAAGGAGGAGCAAGAGAGGCTCAAGAAGCTGGAGGCTGAAAGACTTCGAAGAATGC TTGGAAAAGATGAACATGAAAATAAAAAGAAACCAAAACATACATCAGCAGATGATTTAAATGATGGCTT TATACTGGATAAAGACGATAGGCGCTTGCTTTCTTACAAGGATGGGAAGATGAACATTGAAGATGTCCAG GAAGAGCAAAGCAAGGAAGCAGATGGCCAAGAGAATGACCAGAAAGAGGGAGAAGATGAGAGTGAGGAAG AGGATGAGAGCCATGAGGACTCAGAAGAGAGTGAGGACCCAGACAGCCACTCAGACCTAGAATCTAATAT AGAGAGTGAGGAGGAAAACGAGACACCAAAGAAGGAGCAACGTCAGACTCCTGGGGGGAAGTTGCCAAAG GATGACCAGAAAGCTCAAAAGGCTGTTGCAGCTGAGCTGCCCTATGTCTTTGCAGCTCCTGAATCCTTTG AGGAGCTGAAGTTTTTATTGTCAGGAAGATCAATGGAAGAGCAACTTTTGGTGGTGGAAAGAATTCAGAA GTGCAACCATCCAAGTCTTGCAGTAGGAAACAAAGCAAAATTGGAAAAATTGTTTGGCTTCCTCTTGCAA TACATAGGAGATTTGGCTATAGACAGCACACCGGACCTCAAGACCATCGATAAGTTGGTTATGCACTTAT ATAGTCTTTGCCAGATGTTTCCTGAGTCTGCAAGTGACTCCTTAAGATTTGTTCTCCGAGATGCCATGCA CGAGATGGAAGAAATGATTGAGACCAAAGGCCGGGCGGCGTTTCCAGGGTTGGATGTGCTCATTTATTTG AAAATCACTGGGCTGTTGTTTCCAACCTCTGATTTCTGGCATCCAGTTGTGACTCCTGCCCTGCTGTGTA TGAGCCAGATGCTTACTAAGTGTCCAGTCATGTCCCTACAAGATGTGATAAAGGGCCTGTTTGTGTGCTG CTTATTCCTGGAGTACGTGTCCCTGTCTCGGAGGTTCATCCCCGAGCTATTCAATTTTCTACTTGGGATT CTTTATATAGCAACTCCAAATACAAAGAGCCAAGGTTCCACTCTGGTGCACCCTTTTAGAGCACTTGGGA AGAACTCAGAATTGCTCGTGGTCTCCGACAAAGCAGATGTGGCCACCTGGCAGAGGGGAAGCCTCCCCTT GCACTGGGCAAATAGACTGAGCACCCTGACAGCGACTGAGGCAAATCACACCAGGCTGTCCTGCGTAGCC TTGTGTTTGAGCCTGATGAAGCACTGCGTGCTCATGTATCAGGCACTGCCGTCCTTCCACGCCATCGTCA GGCCCCATCAAGCCCTCCTGGCGAAGCATCTTGCAGATTGTAGCCTCCCCCAAGAACTTAAGGAGCTGGC TCAAAGCATACTGTCAGCAATGGAAGGCCTGAAGCAGCACTGCCGGCCGCTGGTGTGTGAGAAGAGCAAG CCCGTCCCACTGAAGCAGTTCACCCCCCGGCTAGTCAAAGTGCTTGAGTTTGGACGGAAACAAGGCAGCA GTAAAGAAGAACAGGAAAGGAAAAGACTAATCCACAAACACAAGCGGGAATTTAAAGGCGCCGTCCGAGA GATCCGAAAGGACAATCAGTTCCTGGCCAGGATGCAGCTCTCAGAAATCATGGAAAGGGATGCAGAAAGA AAGCGTAAAGTAAAACAGCTTTTTAATAGCCTGGCCACGCAAGAAGGTGAATGGAAGGCTCTTAAGAGGA AAAAGTTCAAAAAATAAATTGTCTTGTAATGTAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5012070 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5012070 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003109 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source