EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31467

Fbxo21 ( MGI:1924223)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006858_01	euxassay_006858_02	euxassay_006858_03	euxassay_006858_04	euxassay_006858_05

euxassay_006858_06	euxassay_006858_07	euxassay_006858_08	euxassay_006858_09	euxassay_006858_10

euxassay_006858_11	euxassay_006858_12	euxassay_006858_13	euxassay_006858_14	euxassay_006858_15

euxassay_006858_16	euxassay_006858_17	euxassay_006858_18	euxassay_006858_19	euxassay_006858_20

euxassay_006858_21	euxassay_006858_22	euxassay_006858_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

strong

regional

strong expression: see section 01 02 03 22 23 moderate expression: see section 21

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 15 16 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 15

forebrain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

spinal cord

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14 15 16

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 16 17

trigeminal v nerve

moderate

regional

moderate expression: see section 08 15 16

thoracic ganglion

moderate

regional

moderate expression: see section 12 13

dorsal root ganglion

strong

regional

strong expression: see section 07 16 17 moderate expression: see section 08 09 10 11 12 15

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 18 19 20

midbrain

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17

vagus x ganglion

moderate

regional

moderate expression: see section 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 16 17 18 19 20

cervical ganglion

moderate

regional

moderate expression: see section 08 15

hindbrain

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6800
Entity Detected:	Fbxo21, ( MGI:1924223)
Sequence:	sense strand is shown >T6800 ACGGCTTCTTAGGCTCCTATCGCGCGCGCGCCCGCCCGCACAGCGGCCCCCTCAGGTACGCGGACAAGAT GGCGTCGGTAGCGGGGGACAGCGCTATGGAGGTGGTGCCGGCGCTGGCCGAGGAGGCCGCGGCTGAGGCG ACGGGCCCCAGCTGCCTGGTGCAGCTGCCGGGCGAGGTGCTGGAGTACATCCTGTGCAGCGGCTCGCTCA CGGCGCTGGACATCGGCCGCGTGTCCAGCACCTGCCGCCGCCTGCGCGAGGTGTGCCAGAGCAGCGGGCA GGTGTGGAAGGAGCAGTTCCGGGTGAGGTGGCCCTCCCTGATGAAGCACTACAGCCCCACCGACTACGTC AATTGGTTAGAGGAATATAAAGTTCGGCAGAAAGCTGGGTTAGAAGCCCGGAAGATTGTAGCTTCCTTCT CCAAGCGGTTCTTTTCAGAGCACGTCCCCTGTAATGGCTTCAGTGACATTGAAAACCTTGAGGGCCCAGA GATCTTTTTTGAGGATGAGCTGGTGTGTATCCTAAACATGGAAGGAAGGAAAGCTTTGACCTGGAAATAC TACGCAAAGAAAATTCTCTACTACCTGCGGCAGCAGAAAATCCTCAATAACCTCAAGGCGTTCCTGCAGC AGCCCGACGACTACGAGTCCTATCTCGAAGGTGCCGTTTACATCGACCAGTACTGCAACCCACTCTCCGA CATCAGCTTCAGAGACATCCAGGCCCAGATCCACAGCATTGTGGAGCTTGTGTGCAAAACCCTCCGTGGC ATCAACAGCCGCCACCCCAGCCTGACCTTCAGGGCAGGTGAGTCCTCTATGATAATGGAGATAGAACTTC AGAGCCAAGTGCTGGACGCTATCAACTATGTCCTGTATGACCAGCTGAAGTTCAAAGGGAACCGCATGGA CTACTATAATGCCTTGAACCTCTACATGCACCAGGTGTTGACCCGCAGAACAGGAATCCCCATCAGCATG TCTCTGCTCTACCTGACCGTCGCCCGGCAGCTGGGGGTTCCCCTGGAGCCTGTCAACTTCCCAAGCCACT TCCTGCTGCGGTGGTGCCAAGGTGCAGAAGGGGCAACCCTGGACATCTTTGACTACATCTACATAGATGC TTTTGGGAAGGGCAAGCAGCTGACAGTGAAAGAGTGTGAGTACCTGATTGGCCAGCACGTGACGGCAGCA CTCTACGGCGTGGTGAATGTGAAGAAGGTGCTACAGCGGATGGTGGGCAACTTGCTGAGCCTGGGCAAGA GGGAAGGCATTGACCAGTCCTACCAGCTCCTCAGAGACTCCTTGGACCTGTACCTGGCGATGTACCCGGA CCAGGTGCAGCTCCTGCTCCTCCAAGCCAGACTCTACTTCCACCTGGGCATCTGGCCAGAGAAGTCTTTC TGTCTTGTCTTGAAGGTGCTTGACATCCTCCAGCACATCCAGACCCTGGACCCCGGGCAGCACGGGGCGG TGGGCTATCTCGTGCAGCATACCTTAGAGCACATTGAGCGTAAGAAGGAGGAGGTGGGCGTGGAGGTGAA GCTGCGCTCCGAGGAGAAGCACAGGGATGTCTGCTACTCCATCGGGCTTGTGATGAAGCATAAGAGGTAC GGCTACAACTGTGTGATCTACGGCTGGGACCCTACGTGCATGATGGGGCATGAGTGGATCCGAAACATGA ATGTGCACAGCCTGCCTCACGGCCACCATCAGCCCTTCTACAACGTCCTGGTGGAGGATGGCTCCTGCAG ATATGCAGCCCAAGAAAACCTGGAATACAACGTGGAGCCTCAGGAAATCTCACACCCAGATGTGGGGCGC TACTTCTCGGAATTCACAGGCACACACTACATCCCAAATGCAGAGCTGGAGATCCGGTACCCCGAGGACC TGGAGTTTGTGTACGAGACAGTGCAGAATATTTACAGTGCAAAGGAGGACACAGCCGAGTAAACTCAGGG GGTGTCACATCTGTGCTGCTGCTGCTGCTGCTGCTGCTGCTACCTTCTAGGGGACCAGGATTCCTGAAGG ACGTGTGCTGCCCCTGGAGTCAGCACAGGACAGCCACTTCACCAGCAGCCTGGCTGCCTCCCCACTTTAG ATGGTGTGCGCTCCTCCAGCCACAGACAGCGTTGCTCCCCGCTACACTAGTGAATCAGTTGAAAGGCACC GTGTCCCGTGGCATGGCTCGTGTGCTTGTCCCGTGGTGACAGTGTGTGACATTCTGTCCTCTCACCCCCT TCCCATCCCTGTCTCCATCTACCTTGTGTCAGTCTCTCCCGCTGGACAGTCAGTCGGGGCCATGTTTCCT CATCTCTGGAACAAGGTCGCTGAGTCAGGCCTGCCGCCCTCTGCCTTCCGTTAGATTGTACTTGGAGGAG GGCATGGAGGTGGGAGTCAGAATGATCACGGTTCAGGTTTTGCTAATACAGAAACACAGATTTCAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3709413 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3709413 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006858 same experiment
	EMAGE:31161 same embryo
	EMAGE:29382 same embryo
	EMAGE:29367 same embryo
	EMAGE:31404 same embryo
	EMAGE:31205 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source