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EMAGE:31475

Nek1 ( MGI:97303)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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euxassay_014225_01 euxassay_014225_02 euxassay_014225_03 euxassay_014225_04 euxassay_014225_05
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euxassay_014225_11 euxassay_014225_12 euxassay_014225_13 euxassay_014225_14 euxassay_014225_15
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 05 06 07 08 12 13 14
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 07 13
facial vii ganglion
moderate moderate
single cellmoderate expression: see section 03 04 05 17 18
trigeminal v ganglion
moderate moderate
single cellmoderate expression: see section 01 02 03 04 05 06 15 16 17 18 19
ventral grey horn
moderate moderate
regionalmoderate expression: see section 07 08 09 11
glossopharyngeal ix ganglion
moderate moderate
single cellmoderate expression: see section 04 05 06 14 15 16
thoracic ganglion
weak weak
regionalweak expression: see section 10 11
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38708
Entity Detected:Nek1, ( MGI:97303)
Sequence:sense strand is shown

>T38708
CAGGTGACGAATACAGTGAGGAGGAAGAGTCTGTTTTAAAAAGCAGCGATGTGGAGCAGACAGCAAGAGG
GACAGATGCCCCAGACGAGGAGGACAACCCCAGCAGCGAAAGCGCCCTGAACGAGGAATGGCACTCAGAT
AATAGTGACGCTGAGACCACTAGTGAATGTGAATATGACAGTGTCTTTAACCATTTAGAGGAACTAAGAC
TTCACTTGGAGCAAGAAATGGGCTTTGAAAAGTTCTTTGAGGTTTATGAGAAAGTAAAGGCTATTCATGA
GGATGAAGATGAAAATATTGAAATTTGTTCAACAATAGTTGAGAATATTTTGGGCAATGAGCACCAGCAT
CTCTATGCCAAGATTCTGCATTTAGTCATGGCAGATGGAGCCTATCAGGAAGATAATGATGAATAATCCT
CAGGACATTCTTTAATAGTCAACTGTAAGAACACATTTGAACTTGGCTCATAATACAAGCTTCCTGGGAA
ATAGAGATTATTTATGAGAAATACCAGATTTAAGATGGGCATGCTTATTGCATGAAAAAAATTAAGAAGT
GTGATATTTTTCCTCACGTTTTATCTACCTTATTTTGTTTATTGGATTCTTCAGCTAACTCTCATAGAAT
GTATGTTTCTTTAAATCACCTCACCAAAGCATTGAGTCAACTGAAAATTTATTGTGCAATAAGGAACTAC
TTTTAGCAACTGTCTTTCTGTGGAAGAGTTGGCTATATCAAAAAAGGCCTGCTTTTGGTGTTTCATT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 109017. Forward Primer - name:109017_F_cDNA_Nek1, sequence:CAGGTGACGAATACAGTGAGGA; Reverse Primer - name:109017_N_SP6_cDNA_Nek1, sequence:AATGAAACACCAAAAGCAGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_014225 same experiment
 EMAGE:31472 same embryo
 EMAGE:30387 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS