EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31476

Oit1 ( MGI:1201784)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002010_01	euxassay_002010_02	euxassay_002010_03	euxassay_002010_04	euxassay_002010_05

euxassay_002010_06	euxassay_002010_07	euxassay_002010_08	euxassay_002010_09	euxassay_002010_10

euxassay_002010_11	euxassay_002010_12	euxassay_002010_13	euxassay_002010_14	euxassay_002010_15

euxassay_002010_16	euxassay_002010_17	euxassay_002010_18	euxassay_002010_19	euxassay_002010_20

euxassay_002010_21	euxassay_002010_22	euxassay_002010_23	euxassay_002010_24	euxassay_002010_25

euxassay_002010_26

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

esophagus

strong

regional

strong expression: see section 10 11 12

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 10 11 12 13 14 16 17 18 19 20

rectum

strong

regional

strong expression: see section 13 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4580
Entity Detected:	Oit1, ( MGI:1201784)
Sequence:	sense strand is shown >T4580 CTAAGAGCAGCCGGCTAGAGAGGGCCAGCACCAGCAACACTACCAACACCAGCACCAGCACCAGCACCAC TCCCATCCTGCCAAGAACCAGAAGGCTGCTCTTCAACAGCCAGGCCAGAAAAGCAGTTGGCTGGGTTAAA GACTCAAGCTATCAACAGCTGATCCATCACCTCTTCAACGCGAAGGCAGTGAAGAGTGAGCCCTGAGCCT CAGGTTAACGCTGACCTGACCGCAACCCATTCTTCACTTCAACAGGATGAGAGTGGCAGGTCTGATCCGT GTTGTGGTCTTCATCTTTACCATAGTCACCATGTGGGTCTTCCTCCGCAGCTACACAAGCTTCAGCAGGA AAACAATTCGTCTGCCACGCTGGCTGGGCATAACCCCCAAGGACATCCAGACCCCGAAGTCCAAGTGTGG GCTCAGCAAGATCTGCCCAAACAACTTTTTTGCATTTAAAATCAGCAGTGGAGCTGCCAATGTGGTGGGC CCCTCCATGTGCTTTGAAGACGAAATTATCATGAGTCCAGTGCGAAACAACGTGGGCAGAGGCCTGAACG TTGCTCTGGTGAACGGATCAACTGGCCAGGTGATGAAAAAGGACAGCTTTGACATGTACTCTGGAGACCC CCAACTCCTGCTGAACTTCCTTACGGAAATTCCAGATAGCACCCTGGTGCTGGTAGCCTCCTATGATGAC CCAGGGACCAAAATGAACGACAAAATCAAGACGCTTTTCTCCAACCTGGGAAGTTCCTACGCCAAGCAGC TGGGCTTCCGGGACAGCTGGGTCTTTGTAGGTGCTAAAGACCTCAAGAGTAAAAGCCCATATGAGCAGTT CTTAAAGAACAACCCAGAAACAAACAAATATGACGGATGGCCGGAGTTGCTAGAGCTCGAGGGCTGTGTG CCACGGAAAGTAATGTAGGGTGGCCTAGGGAGCCAAAGAAATGCCCAACGGACTTGAGAGCAGAACCCAG GAGTGCTGGGTCTATGCTGGCAGGAGCTGACTGGGAAGAAGAAAGAAGCCATATGGTACGCTCTGATCAT CCCCTGCACACAGTGACTCACTTCCCGTCTAAGGAAGCAACAGCCTCCCATTGCCCCATAGTGGCCATCT TGAGGATGGGCGGCCATGGGGTCTGTGGAACCGGCTGTTGGTAGAGAGAACAAGACCTTCCAAGACTGCC ATGGTTGTCTCCAGAAGGACTGGCTGCCTACACTTACTTGAGCAGAATTAAATTCTATTTTTGCTGAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4984632 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4984632 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002010 same experiment
	EMAGE:31725 same embryo
	EMAGE:29890 same embryo
	EMAGE:31441 same embryo
	EMAGE:31865 same embryo
	EMAGE:31204 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source