EMAGE mouse gene expression spatial database

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EMAGE:31483

Stx18 syntaxin 18 ( MGI:1918366)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003103_01	euxassay_003103_02	euxassay_003103_03	euxassay_003103_04	euxassay_003103_05

euxassay_003103_06	euxassay_003103_07	euxassay_003103_08	euxassay_003103_09	euxassay_003103_10

euxassay_003103_11	euxassay_003103_12	euxassay_003103_13	euxassay_003103_14	euxassay_003103_15

euxassay_003103_16	euxassay_003103_17	euxassay_003103_18	euxassay_003103_19	euxassay_003103_20

euxassay_003103_21	euxassay_003103_22	euxassay_003103_23	euxassay_003103_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

lower jaw molar

moderate

regional

moderate expression: see section 20 weak expression: see section 06 07 08

upper jaw molar

moderate

regional

moderate expression: see section 20 21 22 weak expression: see section 06 07 08

foot skin

moderate

regional

moderate expression: see section 05 06 22 23 24

lower jaw incisor

moderate

regional

moderate expression: see section 12 13 16 17 weak expression: see section 14

upper jaw incisor

moderate

regional

moderate expression: see section 12 13 17 18 weak expression: see section 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4717
Entity Detected:	Stx18, syntaxin 18 ( MGI:1918366)
Sequence:	sense strand is shown >T4717 CCACGCGTCCGCCTCAGCAACCGAGTTCTTGCCGGGGTCGCTAGCTGCTGACGTGTGCCTGGCCGGCTTC AGGCCCTGGGTTGCGGGTCGCTATGGCGGTGGACATCACACTGCTCTTCCGGGCTAGCGTCAAGACAGTG AAGACGCGGAATAAGGCTCTGGGAGTGGCCGTGGGTGGCGGGGCCGATGGCAGCCGGGACGAGCTGTTCC GCAGAAGCCCTCGGCCCAAGGGAGACTTCTCCAGCCGGGCCCGTGAAGTGATTTCTCACATTGGGAAGCT GAGAGATTTTCTCCTGGAGCACAGGAAGGAGTATATTAATGCCTACAGCCACACCATGTCTGACTATGGG AGGATGACAGACACAGAGCGAGACCAGATAGACCAGGATGCTCAGATATTCATAAGGACCTGTTCAGAAG CAATCCAGCAACTCAGAACAGAAGGAGTGTGTAAGCTTTACTCTGAACAGAGAGCCATCCGAGTGAAGCG CGTGGTGGACAAGAAAAGACTATCTAAGCTGGAACCTGAGCCACATACCAAGAGGAAAGACTCCACATCT GAGAAAGCCCCTCAGAACGTTTCCCAGGACTCTGAAGGAAAGCCTGCTGCTGAGGAGCTGCCAGAAAAGC CTTTGGCTGAATCACAGCCCGAACTGGGAACCTGGGGCGATGGCAAGGGTGAAGATGAGCTGTCTCCAGA AGAGATACAGATGTTTGAACAAGAAAATCAGCGACTCATTGGTGAAATGAACAGCTTGTTTGACGAAGTG AGGCAAATCGAAGGGAAAGTCGTTGAAATTTCCAGACTCCAAGAGATATTCACCGAAAAGGTTTTGCAAC AGGAAACTGAGATCGACAGCATTCACCAGTTAGTCGTGGGGGCAACTGAAAATATTAAGGAAGGCAACGA AGACATCCGAGAGGCCATCAAAAACAATGCAGGTTTCCGAGTGTGGATCCTCTTCTTCCTCGTGATGTGT TCCTTTTCCTTGCTCTTCCTCGACTGGTACGACAGCTAGCCCACCAGGGCTGTGTGCCTGCGCCTGCGCC TGCGCCTGTGCCTTGGACGCACCACGTGCCAGCATGTGTCTCATCGGCTGTGGAGCTGTGTAAGGTCCAA GGGCTGGGAAAACACCGCCCCCAAGCCAACCTTTGGAACCCCTCAGGACCTGGAAATACATGGATTGCAA GTGCCCTTCAGAGTGGAAAATAAAAGGCCATCAGGAGCAGCACCTTCAGGACAGGCCACAAGCATGGTGG GGACTGAGCAGAGCAAGGAAATGGCAGGCCTCCTATCTCCATTACAGGCTTGGGGCCCATCTCCTGCCCT TCCCACGCCCGTAGCTTCAGGGGAGAACATGCCTAATGTTGCTAAAGTCATCAGTTCTTATTTCCTTGTG GCATCTAATGTGTGCTGATATATGGCCATTTTGTCATAGACAATAATTCACATCTGCCTTGTCGAGCTAA TAAATGCAATTACAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5035350 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5035350 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003103 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source