EMAGE mouse gene expression spatial database

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EMAGE:31486

Aph1b ( MGI:3522097)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005760_01	euxassay_005760_02	euxassay_005760_03	euxassay_005760_04	euxassay_005760_05

euxassay_005760_06	euxassay_005760_07	euxassay_005760_08	euxassay_005760_09	euxassay_005760_10

euxassay_005760_11	euxassay_005760_12	euxassay_005760_13	euxassay_005760_14	euxassay_005760_15

euxassay_005760_16	euxassay_005760_17	euxassay_005760_18	euxassay_005760_19	euxassay_005760_20

euxassay_005760_21	euxassay_005760_22	euxassay_005760_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 17 18 19 weak expression: see section 06 07 08

cerebral cortex

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 03 04 05 06 07

spinal cord

moderate

regional

moderate expression: see section 07 08 09 10 11 12

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

trigeminal v nerve

moderate

regional

moderate expression: see section 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9373
Entity Detected:	Aph1b, ( MGI:3522097)
Sequence:	sense strand is shown >T9373 CGCCACCCAGCCCCGCCCCGTATCCAGGACCCGCTGTCCATTGGGCACTGCAGGGCCCTCGGGCCCCCGC GACTGGCTCCCGAGGTCGTCATGACGGCTGCGGTGTTCTTTGGTTGCGCCTTCATCGCCTTCGGGCCCGC GCTCGCTCTTTATGTCTTCACCATCGCCACCGACCCTTTGCGAGTCATCTTCCTCATCGCCGGTGCTTTC TTCTGGTTGGTGTCTCTCCTGCTGTCATCCGTTTTTTGGTTCCTAGTGAGAGTCATTACTGACAACAGAG ATGGACCAGTACAGAATTACCTGCTCATCTTCGGAGTGTTGCTCTCTGTCTGTATCCAAGAGCTGTTCAG GCTCGCATATTATAAGCTGTTAAAAAAAGCCAGTGAGGGTTTGAAAAGCATAAACCCTGAGGAGACAGCA CCCTCGATGCGACTGTTGGCCTATGTTTCTGGCTTGGGCTTTGGAATCATGAGTGGAGTGTTTTCCTTTG TGAACACCCTGTCTAACTCCTTGGGGCCAGGCACAGTGGGCATTCATGGTGATTCTCCTCAGTTCTTCCT TAATTCAGCTTTCATGACACTGGTCGTCATAATGCTGCACGTGTTCTGGGGCGTTGTGTTCTTTGATGGC TGTGAGAAGAATAAGTGGTACACCCTCCTCACGGTTCTCCTGACGCATCTGGTGGTGTCCACCCAGACTT TTCTAAGTCCTTATTATGAAGTGAATCTGGTAACAGCATATATAATCATGGTGCTCATGGGCATCTGGGC ATTTTATGTTGCCGGAGGCAGCTGCCGAAGCTTGAAACTCTGCCTGCTCTGCCAAGACAAGGATTTTCTT CTTTACAACCAACGCTCCAGATAACCTCTGACCATCAGCACCTCTAAGCAGCAGCCTGTGTCTTTAGAGG AAGCACAACTGGGCCCTGTTCTAAAAAGACCCCTGTATCTTGGAACTTGGAAGCCACAGAACTGTCCAGC CTGGAACTGTCCTGTGCTTTCATGCAGCAGCCTCTGGAAGAGCTGCAGTGTAGCCTGCAGCCTGGCTGCA CATTTGAAGCATCTGGGGAAACAAGGATTGATGCTTCTGGGCTTCTCCAGACTTGATTATCCTGGATGTG CAGGACAGCTGAGAGTCACTGGGGTCCAATGGGGAGTTCGAGAAAACACCCAAAAGCTGTTCAGTGTTGG TCTTCTAAACCAACTTCAGCTGAGCAAGTAAATATCTGAATGTGGTAACCAGCGTAGCCCCTGTCTTTTT ATGTACTGATGTCACAGACTCCAGGGCTGTTCTGTCTGTGGATGGGTTTGCTAGCCTTCACCAGCATCAT AGGCTGTGCCTTTAAATGCAGTAACCTACACTGGCATTATAACTTAGCCAAGGTAAGGTCCATGTAGAAC CTGTATTTTTCTCATTATTTTATCAATTTTAAGTTTTTCTTCCTCAGGCACTAGATTATTTATTTGAGTA TAAATTCAGGCTCTTTAGGATTAATTATATGCTGACTTCCTTGGGAGAGTCTAAAATTATGTGCTGAATT CCTTGGAAGAAATTAGCAGTGCAATTTCTTTGGATTCCGTAAGTTTATTCATTATAAAATACTAACAGCA ACATAGATTCCTCAGTTTTAAGCTGCTGTGTGTGCTGTCTGAAACTGGCTTGTCTAGGGAAAACTCACCT TTTTTATCTCTGAGAAGCTGGGCAGGGAGATGAATGATGGACCTTTGGGAGATCTTCTCTTTGTGAGAGT GTAGCTAAACCACAAAGTAGTGTGACATTGACACTTCTCATTTGCTACTGTGACACCAAACAGTGCCATC ACCCTAATGGTACAGGGTAAAGTTGGAGATAGACCAAATTTCAAGCTGGCATGGAACTTTTCATCTTCAG CAGTTCGCCTGGGTTCTGGTTACTGTGAGACAAACAGCATTCCCTTTTCAGGACTACGCAAGGGCTTTGT TAGTCTTCCAGATAAGGCCTCTGGCTTCCTCTGTAAGAGGAATGCTACTGCATTGGGAGTCTGCTGTGGA GACTGTTCCCAATCCAAGTCTTTTGGGAACAGAGTGTAAAGTAGCAATGACCTCTTGGTGGAAGTGATGC CTTGGTGATCCCAGCACACATGTGCACTGTCTATAAGAACCAGGGGCAGCAGTGAGAGGGTTTGGCAGTG ACACTTAAGATGAATTACCCAGGAACTAAACCTTAATTGATGAACTTCCACCATGCTTAAAATATTTGAG TGTCTATGTAGAGATGCAAGACTTATAAAGCTTCTAGCAAGAATATAGATTACCTGAGAAACCACTGTTT CTCCTTAAAGGTTACTTCAGCTCTAAATCATGTTAGTGCCTCTGCTGGAGTTTATTGGTTGTATCTTCAT ATAAGATTTCCCCTGGACTAGAAGGACAAAGACATAGAAAGCAAACAGTAAACTTCACTTTACAGAGCCA AGAACAAGAAGATGCCCACAGGGCTGGGATGTGTTCCTCCCTGAGCATGGTTACTACTGCTGTCTTATAC ACCTTGTATGGAGATATTCCGATAAAATGTTTGCTTGTCCGAGGCATGGAGAACCTGAGAATTTGACTGT TATTATACTTGAGCTCTAAGCAAATTCTCTGAGTATACCACAATATTAGAAATGAGGCTATTATACAATC TCAATTAATTAAAAAAACTCAGATGAATGGCTGGGAAGATGGCTCACTGGCTAAGCACACATACTGCTCT TTCAGAGGATCCAAGTTCAGTTCCAAGAACCCCCTTTGAGCAGCTCACAACCACTTCTAACTACAGCTCT AGGAGATCCAATGCATCTGGCCTTCAACTGCGACTGTATTCATATGCCCACACAGGCAGGACCATACATA TTCAAAAACAGAACCAACACACATGAGAAGGAAATTTGCCTAGTGAAACTCAGTTGAATTATTGAATAGT CCTTGTTTGCCTGTGGTTGTTCTTATACTGTGAGTGCTGCTGTGTAGCTCAGGCAGAGCTGTTTCCGTAG CCTGCTTTGCACCTTACCTAGGAAGCCTGAGGGAGGTGGAGTCTTACACATACACATTGAAGTGCTGCTC TTATTAATAATTGAAAAGGAGTAGCCTGGATGTTGTATGCTGTGTTCCACACAGGTTTTCTGTGAAGAGA GAGAAAATGCACATAAAATTGACCTTGGGATTTTGAATATTGTTTTAGTGCTTGAGTAACAGAGATGCAT TGACCATGTGAATAGCCGGGATAGATTCAGTTGTAATTTCACATAAGCCACATTTATCCTTAGTTTGTTT GAACCAGTGCTCTTCACTGGAGGTGTGTCCACCTGTCTGTCCTTTCGCTTGCCGGGCTAGCAGTGCAGCA GTAGTGACATTTTCAGAACATCTTGATGAAATGTGCTCCAGGGAAGTGAGGACAGTGTCTTCAGACTGCT CAGAAGATAGCTTTTGTTCCCAGTAAACATAAGTACTGTGAGAGAGATTTTTTTCTCTCCCTAGTTCTTG TTTCAGTAGCTTCAGCCCTGTGTCAAAACAGGTGTTAACAGTAGTTGTGAGCACTGAAGCCTTGCTAGGG CTTATAGGCATGTGCCACCACGCCTAGCTAAATGATCTCATTATGATTACAATATTTATCTTTTTGTTTA ATAACAGGCTTTACTTTAGTCCTTTCTAATGTTTAGAGGAAATGGACACCATCTCTCTGTATCATCTGTA ACATTTTTACATGTTAACCCTTTATGACCCGTATGACCTGCCTTTATGACCTGTAGCAGCAAACCTTCCA GGCCTGTGTTTTTCCCAATGGACAGCAAAAACATTATTTAACATTTCATTCTCTAGATATCCCTCATTTG GAGTAGACCATCTTTCAGTATCTGTTAGTATAGTTATTGCCTTGTTTGAAACTTGGCTGTACATTCTGTT GTTTGTTACTGATTAGTTGCGAGACTTTCAAAGTGAAGTTCATTAGCTAATTTACAACCCACAACGCATG TATCATTTCTCTGTATTCAGAATCAATTTGGCACAAAGATGTTCCTTGTGGCTTCTTAATCCAAGTAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6853991 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6853991 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005760 same experiment
	EMAGE:30394 same embryo
	EMAGE:30383 same embryo
	EMAGE:30360 same embryo
	EMAGE:30370 same embryo
	EMAGE:31478 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source