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EMAGE:31513

Kcnk1 ( MGI:109322)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31513 EMAGE:31513 EMAGE:31513 EMAGE:31513 EMAGE:31513
euxassay_001701_01 euxassay_001701_02 euxassay_001701_03 euxassay_001701_04 euxassay_001701_05
EMAGE:31513 EMAGE:31513 EMAGE:31513 EMAGE:31513 EMAGE:31513
euxassay_001701_06 euxassay_001701_07 euxassay_001701_08 euxassay_001701_09 euxassay_001701_10
EMAGE:31513 EMAGE:31513 EMAGE:31513 EMAGE:31513 EMAGE:31513
euxassay_001701_11 euxassay_001701_12 euxassay_001701_13 euxassay_001701_14 euxassay_001701_15
EMAGE:31513 EMAGE:31513 EMAGE:31513 EMAGE:31513 EMAGE:31513
euxassay_001701_16 euxassay_001701_17 euxassay_001701_18 euxassay_001701_19 euxassay_001701_20
EMAGE:31513 EMAGE:31513 EMAGE:31513 EMAGE:31513
euxassay_001701_21 euxassay_001701_22 euxassay_001701_23 euxassay_001701_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
neural retina
strong strong
regionalstrong expression: see section 24 moderate expression: see section 02 03 04 05 23
metencephalon rest of alar plate mantle layer
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 19 20
ventral grey horn
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14
stomach
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10
kidney calyx
strong strong
regionalstrong expression: see section 04 05 06 07 08 13 14 15 16
rib
moderate moderate
regionalmoderate expression: see section 02 03 04 05 18 19 20
rectum
moderate moderate
regionalmoderate expression: see section 11 12
urethra of male
moderate moderate
regionalmoderate expression: see section 11 12 13
medulla oblongata alar plate mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16 17
pons mantle layer
moderate moderate
regionalmoderate expression: see section 09 10 11 13 14 15 16 17 18
cochlea
strong strong
regionalstrong expression: see section 06 07 08 17 18 19 20
midgut
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15 16 18
bladder
moderate moderate
regionalmoderate expression: see section 11 12 13
pancreas
moderate moderate
regionalmoderate expression: see section 07 08 09 10
cornea stroma
weak weak
regionalweak expression: see section 23 not examined expression: see section 02 03 04 05 24
submandibular gland primordium
strong strong
regionalstrong expression: see section 07 08 09 10 17 18 19 20
cerebral cortex marginal layer
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
trachea
moderate moderate
regionalmoderate expression: see section 13
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20
hindgut
moderate moderate
regionalmoderate expression: see section 12 13 14
upper lip
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 16 17 18
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19
diencephalon lateral wall mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 14 15 16 17 18
lung
moderate moderate
spottedmoderate expression: see section 02 03 04 05 06 07 08 09 12 13 14 15 16 17 18 19 20
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1344
Entity Detected:Kcnk1, ( MGI:109322)
Sequence:sense strand is shown

>T1344
CCTCGAGACTGTTGGCCTACTGGCTCTTGTATTTGCACGAAACCAGGAAAGGGAGCGGCGGCGGGAGGCG
CGGAGCGCGGGCGCCGGGAAGATGCTGCAGTCCCTGGCCGGCAGCTCGTGCGTGCGCCTGGTGGAGCGGC
ACCGTTCGGCCTGGTGCTTCGGCTTCCTGGTGCTGGGCTACCTGCTCTACCTGGTGTTCGGCGCCGTGGT
CTTCTCGTCCGTGGAGCTGCCTTATGAGGACCTGCTGCGCCAGGAGCTGCGCAAGCTGAAGCGGCGCTTC
CTGGAGGAGCACGAGTGCCTGTCGGAGCCGCAGCTGGAGCAGTTCCTGGGCCGCGTGCTGGAGGCCAGCA
ATTATGGAGTGTCGGTGCTCAGCAACGCCTCGGGAAATTGGAATTGGGACTTCACCTCGGCGCTCTTCTT
CGCCAGCACCGTGCTCTCCACCACAGGCTATGGCCACACGGTGCCCCTGTCAGATGGGGGCAAAGCCTTC
TGCATCATCTACTCTGTCATCGGCATCCCGTTCACCCTCCTCTTCCTGACGGCCGTGGTCCAGCGTGTCA
CCGTGCATGTCACCCG
Notes:The probe template was PCR amplified from IMAGE:2286404 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2286404 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_001701 same experiment
 EMAGE:29573 same embryo
 EMAGE:29572 same embryo
 EMAGE:31520 same embryo
 EMAGE:31974 same embryo
 EMAGE:29811 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS