EMAGE mouse gene expression spatial database

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EMAGE:31520

5730596K20Rik ( MGI:1924270)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001705_01	euxassay_001705_02	euxassay_001705_03	euxassay_001705_04	euxassay_001705_05

euxassay_001705_06	euxassay_001705_07	euxassay_001705_08	euxassay_001705_09	euxassay_001705_10

euxassay_001705_11	euxassay_001705_12	euxassay_001705_13	euxassay_001705_14	euxassay_001705_15

euxassay_001705_16	euxassay_001705_17	euxassay_001705_18	euxassay_001705_19	euxassay_001705_20

euxassay_001705_21	euxassay_001705_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 10 15 16

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 05 06 07 19 20 weak expression: see section 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1403
Entity Detected:	5730596K20Rik, ( MGI:1924270)
Sequence:	sense strand is shown >T1403 CCTCGAGCCTGTTGGCCTACTGTTGCTCTAAGCACTATGACTAGTTCTGTTCAGATTTACAATTTGTCCC AGAATATTCAGGAAGATGATCTTCAGCAGCTACAGCTCTTCACAGAATATGGCCGCCTGGCAATGGATGA AATTTTCCAAAAACCTTTTCAGACACTGATGTTTTTGATTCGAGATTGGAGTTTCCCTTATGAATATAAC TATGGACTGCAAGGAGGAATGGCATTTTTGGATAAGCGTTTACATGTGAAGGAACATCAACATGAAGAAA TTCAGAATGTGAGAAATCATATTCACTCATGTTTCTCCGATGTCACCTGTTTTCTCTTACCACATCCAGG GCTCCAGGTGGCCACAAGCCCCAACTTTGATGGGAAATTGAAAGATATTGCTAGTGAATTCAAAGAGCAG TTACAGGCTCTAATACCATATGTACTAAATCCATCTAAATTAATGGAGAAGGAGATCAATGGTTCAAAAG TCACCTGCCGGGGATTGTTGGAATATTTTAAGGCATATATTAAAATTTACCAAGGAGAAGATCTGCCTCA CCCGAAGTCCATGCTTCAGGCCACTGC
Notes:	The probe template was PCR amplified from IMAGE:2372832 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2372832 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001705 same experiment
	EMAGE:29573 same embryo
	EMAGE:29572 same embryo
	EMAGE:31513 same embryo
	EMAGE:31974 same embryo
	EMAGE:29811 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source