EMAGE mouse gene expression spatial database

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EMAGE:31576

Klhl20 ( MGI:2444855)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005048_01	euxassay_005048_02	euxassay_005048_03	euxassay_005048_04	euxassay_005048_05

euxassay_005048_06	euxassay_005048_07	euxassay_005048_08	euxassay_005048_09	euxassay_005048_10

euxassay_005048_11	euxassay_005048_12	euxassay_005048_13	euxassay_005048_14	euxassay_005048_15

euxassay_005048_16	euxassay_005048_17	euxassay_005048_18	euxassay_005048_19	euxassay_005048_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 11 12 13 14

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 13 14 weak expression: see section 07 08

facial vii ganglion

moderate

regional

moderate expression: see section 03

vagus x ganglion

moderate

regional

moderate expression: see section 07

thymus primordium

moderate

regional

moderate expression: see section 09 10 11 12 13

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 06 07 08 15 16 17 18 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 15

pons mantle layer

moderate

regional

moderate expression: see section 15 weak expression: see section 06

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7450
Entity Detected:	Klhl20, ( MGI:2444855)
Sequence:	sense strand is shown >T7450 ATCCTGCCTGCGCTAGCGGCTCCAGAGGTGGAGGCAGAGAGGGCGGAGAAGGCAGAGAGGGTGCCGCAGA GAGAAAAGTGTCAAAGCCAGTACGGCTTTAGAACACGGTGAAGGATACTTTTCATGGTGCAGGGAAGGAA AGCCAATGCGCAGGTGTACCAACATTCGACCAGGAGAGACTGGAATGGATGTAACAAGCCGCTGCACCCT TGGAGACCCCAACAAGCTGCCAGAAGGGGTTCCCCAACCTGCTCGCATGCCCTATATTTCAGACAAGCAC CCTCGGCAAACCTTGGAAGTGATCAACCTTCTGAGAAAGCACCGGGAGCTATGTGATGTGGTGCTAGTTG TGGGCGCTAAGAAGATATATGCCCACCGCGTCATCCTGTCAGCCTGCAGCCCCTACTTCCGAGCAATGTT TACAGGGGAGCTGGCAGAAAGCCGGCAGACAGAAGTAGTGATCCGAGACATAGATGAGAGGGCCATGGAG CTCCTGATTGACTTTGCATATACCTCCCAGATCACTGTGGAAGAGGGCAATGTTCAGACTCTCTTGCCTG CTGCTTGCCTCCTCCAGCTGGCAGAAATACAGGAAGCCTGCTGTGAATTCTTAAAGAGACAATTAGATCC TTCTAATTGTCTGGGCATTCGGGCTTTTGCTGACACACATTCTTGCCGTGAGTTGCTAAGGATTGCAGAC AAGTTCACCCAACATAACTTTCAAGAGGTAATGGAGAGTGAAGAATTCATGTTACTTCCGGCCAACCAAC TCATTGACATAATATCTAGTGATGAGCTCAATGTTCGGAGTGAAGAACAAGTGTTCAATGCAGTGATGGC CTGGGTCAAATACAGTATTCAGGAAAGACGGCCTCAGTTACCCCAGGTGCTGCAACATGTTCGCCTGCCT TTGCTTAGTCCCAAGTTCCTGGTGGGCACCGTAGGCTCTGACCCCCTCATTAAAAGTGATGAAGAATGCA GAGACTTGGTAGATGAGGCTAAAAACTACCTCCTGTTGCCTCAAGAACGACCACTAATGCAAGGGCCAAG GACAAGACCACGGAAACCTATCCGATGCGGAGAAGTACTCTTTGCAGTTGGTGGTTGGTGCAGTGGAGAT GCCATTTCCAGTGTTGAACGCTATGACCCCCAGACCAATGAGTGGCGAATGGTAGCTTCAATGAGCAAAA GGCGATGTGGAGTCGGGGTCAGTGTTCTTGATGACCTGTTATATGCAGTAGGCGGCCATGATGGATCCTC ATATCTCAATAGTGTTGAAAGATATGACCCCAAAACTAACCAATGGAGCAGCGATGTGGCCCCTACAAGC ACCTGCAGGACAAGCGTTGGTGTGGCAGTCCTTGGAGGTTTTCTCTATGCTGTGGGTGGACAGGATGGTG TCTCTTGCCTCAATATAGTTGAGAGGTATGATCCAAAGGAGAACAAGTGGACCCGGGTGGCTTCAATGAG CACTAGAAGGCTAGGGGTGGCTGTCGCCGTGTTGGGAGGGTTCTTATACGCTGTTGGTGGCTCAGATGGA ACATCTCCACTTAACACAGTGGAGCGCTACAATCCTCAGGAAAACAGATGGCACACCATAGCGCCTATGG GGACCCGAAGAAAACACCTAGGCTGTGCGGTGTACCAGGACATGATCTATGCAGTGGGAGGGAGAGACGA CACTACAGAGCTTAGCAGTGCTGAGAGATACAACCCCAGAACTAACCAGTGGTCTCCCGTGGTGGCCATG ACATCCCGCAGGAGTGGAGTCGGCCTGGCCGTGGTCAATGGACAGCTTATGGCAGTGGGGGGCTTTGATG GCACAACATACTTGAAGACCATTGAAGTTTTTGACCCAGATGCCAATACGTGGAGGTTATACGGAGGGAT GAATTACCGTCGGCTAGGAGGTGGTGTAGGAGTTATTAAAATGACACATTGTGAATCCCATATATGGTGA ACACAGGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4235202 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4235202 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005048 same experiment
	EMAGE:30450 same embryo
	EMAGE:31583 same embryo
	EMAGE:31585 same embryo
	EMAGE:30522 same embryo
	EMAGE:30467 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source