EMAGE mouse gene expression spatial database

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EMAGE:31590

Rbm15b ( MGI:1923598)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_004160_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7821
Entity Detected:	Rbm15b, ( MGI:1923598)
Sequence:	sense strand is shown >T7821 GGCGGCGCCGGGGGCGCTGCCTCCTGGGCCGCCGCCGCCGCCGCTCTGAGGGACCCGCGGGCCGCCCGCC CGCCCGCCGAACCGGCGCGATGAAGCGGCAGAGCGAGCGAGACTCCAGCCCGAGCGGGCGTGGCTCGTCA TCGTCCGCCCGTACGGCTACCAGGCCGTGTGCGAGGAGGACCTGATGCCGGAGGATGATCAGAGAGCCAC TCGAAACCTCTTCATCGGCAACCTGGACCACAGTGTTTCTGAGGTGGAGCTTCGACGGGCCTTCGAGAAG TACGGCATCATCGAGGAGGTGGTCATCAAGAGGCCTGCCCGCGGCCAGGGTGGTGCCTATGCCTTCCTCA AGTTTCAGAATCTGGACATGGCACACAGGGCGAAGGTGGCTATGTCTGGCCGGGTGATTGGCAGAAACCC CATAAAGATAGGCTACGGCAAGGCTAACCCCACCACCCGCCTCTGGGTGGGTGGTCTCGGACCTAACACC TCGCTGGCGGCCCTGGCCAGGGAATTCGATCGCTTCGGGAGCATTCGGACCATCGATCACGTCAAAGGAG ACAGCTTTGCCTACATCCAGTACGAGAGCCTGGACGCAGCCCAGGCTGCCTGTGCTAAAATGAGGGGCTT CCCCTTGGGTGGTCCAGATCGCAGGCTCCGGGTGGATTTTGCCAAAGCCGAGGAGACTCGCTATCCCCAG CAGTACCAGCCCTCACCTCTCCCTGTGCACTATGAGCTGCTCACTGACGGATATACCCGGCATCGGAACT TGGATGCTGACCTTAGGGTGCGGGATAGGACCCCTCCACACCTTCTGTATTCAGACCGAGACCGGACCTT TTTGGAAGGGGACTGGACCAGCCTCAGTAAAAGTTCAGACCGCAGAAACAGCCTGGAGGGCTATAGCCGC TCAGTTCGCAGCCGGAGTGGTGAGCGCTGGGGGGGTGATGGGGACCGGAGCATAGCCAAGCCCTGGGAAG AGAGACGCAAGCGGAGGAGCCTCTCCAGTGACCGTGGGAGGACAACTCACTCCCCTTACGAGGAACGCAG CAGGACCAAGGGTGGGGGGCAGCAGTCTGAGCGAGGCTCAGATCGCACCCCTGAGCGTAGCCGCAAGGAG AACCACTCCAGTGAAGGGACCAAGGAGTCAGGCAGCAACTCCCTCAGCAACAGCAGACATGGTGCTGAGG AGAGGAGCCACCATCACCACCACCATGAGGCTCCAGACTCTTCCCATGGGAAAAAGACTAGAGAGAGTGA ACGCAATCATCGGACCACTGAGGCAGAGCCCAAGACTCTTGAAGAGCCAAAACACGAGACCAAAAAGCTA AAGACTCTGTCAGAATATGCCCAGACACTGCAGCTGGGTTGGAATGGGCTTCTGGTGTTGAAAAACAGCT GCTTTCCGACATCTATGCACATCCTTGAGGGGGACCAGGGAGTTATCAGTGGCCTCCTCAAAGACCATCC TTCTGGCAGCAAGCTGACCCAGCTAAAGATTGCCCAGCGCCTTCGACTGGATCAGCCCAAGCTGGACGAG GTCACCCGGCGAATCAAGCAGGGGAGCCCTAATGGCTATGCTGTGCTCCTGGCCATCCAGTCAACCCCCA GCGGGCCTGGCGCCGAGGGCATGCCCGTGGTGGAGCCAGGCCTACAGAGGCGGCTTCTCAGGAACCTAGT CTCCTACTTGAAACAGAAGCAGGCTGCAGGGGTGATCAGCTTGCCGGTGGGTGGGTCTAAGGGCAGAGAC AACACAGGCATGCTCTATGCCTTCCCGCCCTGCGACTTTTCACAGCAGTACCTCCAGTCAGCGCTGCGGA CATTGGGCAAGTTAGAGGAAGAACACATGGTGATAGTTATAGTAAGGGACACCGCCTAGCCCGCACCTGT GCTTTCCAGCTGTGTTTGTGTCACAGAAGCAGTTATTTTAAAGATCTGCTCCTTCTCCACCTACCTTCTC CCTTGGTTTGAATTCTCTGCTGGGTTTTTGTGGTTCATTTGGTGGCATCCTGTCTACTGCTAAAACTAAG TTCTTAGATTTGGGGGGAATTTTTGTTTTTTTATAAGAAGAAACTTATGTTTCCCGTGTATGAGATTCTG TCTGCTGTATTCTGTATTTTTTTATATTTTATTTTATTTTTTTGACTAACTGTATGGAAAATTGTCAGTA AAGCCTTTGTCAGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6332977 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6332977 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004160 same experiment
	EMAGE:32248 same embryo
	EMAGE:31652 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source