EMAGE mouse gene expression spatial database

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EMAGE:31591

4933409I22 ( MGI:3606076)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006340_01	euxassay_006340_02	euxassay_006340_03	euxassay_006340_04	euxassay_006340_05

euxassay_006340_06	euxassay_006340_07	euxassay_006340_08	euxassay_006340_09	euxassay_006340_10

euxassay_006340_11	euxassay_006340_12	euxassay_006340_13	euxassay_006340_14	euxassay_006340_15

euxassay_006340_16	euxassay_006340_17	euxassay_006340_18	euxassay_006340_19	euxassay_006340_20

euxassay_006340_21	euxassay_006340_22	euxassay_006340_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

choroid invagination

strong

regional

strong expression: see section 05 06 12 13 17 18 moderate expression: see section 07 08 09 10 11 14 15 16

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 05 06 13 17 18 moderate expression: see section 01 02 03 04 07 08 09 10 11 12 14 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9696
Entity Detected:	4933409I22, ( MGI:3606076)
Sequence:	sense strand is shown >T9696 GCCTCAGGGTCGCGGCCGAAGTGAAGCGGGACAGCTTACTGAAGGCTCTCAAAGCACCCTAGGCTGAGTG ATGTCTGAGGAAGACTCGGACAGCGTGATCACTGACTCCCAGGCGGAGGAGGAAGGCGAGCTGCCAGTCA TCCAACTGTGCGGGCTGGTAGAGGAGCTCAGCTATGGAAACTCTGCTCTCAAAACAGAAACAGAGATGTT CGAGAAATATTACTCTAGACTGGAACCCAGGGACCACCGAATGACACGGTTGTCAGATATTAAAATAACA GCAGCAGAATTTTCACAGTTACGATCCAGGCGTAAATCCAAGGCCCGTGGAGGTCTGGACCGTACGCTAG GCCTCACTGTAGAGCAAAAGCTCGAGCTTGTGCAGAAGGAGCTGGAAGACACGAGAGATGAAATACGGCA CATGCGGGCTAATGCGGAACGGGACCTGCAGCATCACGAGGCTATCATCGAGGAAGCCGACATTCGGTGG AATGAAGTTCAGAAAGCAGTGCACGAATTTGAAAAAGATATTCTGAAAACCATATCCAAGAAGAAAGGGA GCATTTTGGCCACTCAGAAAGTGATGAAATACATCGAGGACATGAACCGCCGCAGGGATAATATAAAGGA TAAATTGTGTTTGAAAAATGTTTCTCTCAAAGTTCAAAGGAAAAAGATGCTTTCACAATTGAGACAGAAG GAGGAGGTAGGCGAGGCCCTGCACGACGTGGACTTTCAGCAGCTGAAAATCGAGAACGCTCAGTTTTTGG AGACGATTGAAGCAAGGAACAAAGAACTGATCCAGCTGAAGCTGGCCTCTGGGAACACGCTGCAGGTTCT CAACACCTACAAAAACAAGCTTCATCGGGCAATGGAGATCTACGTCAATCTGGACAAGGAGATCTTGCTG AGAAACGAGCTACTTGGGAAAATTGAAAAAGAAACCATACAAGCAGAGGAGGACAGGGCCAAAGCCCATA TCTTGAACGATAAGTTACGGAAGCAGCTGGCTGAGTTCCGGGCCCCACAGGTAATGATGTACGTCAAGGA GAAGATCCTGAATGGGGAGCTAGAGAAGACCATCAAGATGTGGGAGCGGAAGGTGGAGATCGCAGAGATG TCTCTAAAAGGGTACCGGAAGGCCTGGAATAAGATGAAGACTTCGGATGAGCAGTTGCAGATAATCCGGC CCCCTGGGAAATAGTCAGGAGCAGCCGGCAGCTTATAGACCAAGAAGTGACCCATTAAAGTTCTCTATTA TTTTCCGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6705542 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6705542 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006340 same experiment
	EMAGE:31529 same embryo
	EMAGE:30120 same embryo
	EMAGE:30480 same embryo
	EMAGE:29565 same embryo
	EMAGE:30477 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source