EMAGE mouse gene expression spatial database

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EMAGE:31629

Slc25a14 ( MGI:1330823)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004145_01	euxassay_004145_02	euxassay_004145_03	euxassay_004145_04	euxassay_004145_05

euxassay_004145_06	euxassay_004145_07	euxassay_004145_08	euxassay_004145_09	euxassay_004145_10

euxassay_004145_11	euxassay_004145_12	euxassay_004145_13	euxassay_004145_14	euxassay_004145_15

euxassay_004145_16	euxassay_004145_17	euxassay_004145_18	euxassay_004145_19	euxassay_004145_20

euxassay_004145_21	euxassay_004145_22	euxassay_004145_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

dorsal root ganglion

weak

regional

weak expression: see section 06 07 13 14 15

midbrain

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

diencephalon

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 16 17 18 19 20

cervical ganglion

weak

regional

weak expression: see section 07 15

hindbrain

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

cervico-thoracic ganglion

weak

regional

weak expression: see section 14

spinal cord

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15

not examined

regional

not examined expression: see section 06

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7823
Entity Detected:	Slc25a14, ( MGI:1330823)
Sequence:	sense strand is shown >T7823 CGGTGGCTTTACTTCTTCGATTGAGCTCTGCTTCCTCGACCCCCCGGGAGGCCGCCTCCTTCAGGCGCCT CCCTTCTCTCTACGAACTCGCTCTGACAGCCGAGGAACTGGCAAGATCCTGCACCCAGAGGGTGAATGGG TATCTTTCCCGGAATAATCCTAATTTTTCTAAGGGTGAAGTTTGCAACGGCGGCAGTGATTGTAAGCGGA CATCAGAAAAGTTCCACTTTAAGCCATGAGATGTCTGGTCTGAACTGGAAACCTTTTGTGTATGGCGGCC TTGCCTCTATTGTTGCCGAGTTCGGCACTTTCCCTGTGGATCTTACTAAAACACGGCTGCAAGTCCAAGG CCAGAGTATCGATGTTCGTTTCAAAGAAATAAAATATAGAGGGATGTTTCATGCCTTGTTCCGAATCTAT AAAGAAGAAGGGATCTTGGCTCTGTATTCAGGAATTGCCCCTGCGTTACTAAGACAGGCATCATATGGCA CCATCAAAATTGGTATTTATCAAAGCTTGAAGCGATTATTTGTAGAACGTTTGGAAGATGAGACTCTCCT AATTAACATGATCTGTGGGGTAGTGTCAGGAGTGATTTCCTCTACTATTGCCAATCCCACTGATGTTCTA AAGATTCGAATGCAGGCTCAAGGAAGTTTGTTCCAAGGGAGCATGATTGGCAGCTTCATTGACATATACC AGCAAGAAGGTACCAGGGGTCTGTGGAGGGGTGTGGTCCCAACTGCTCAGCGTGCTGCAATCGTTGTGGG AGTAGAGCTGCCCGTTTATGATATTACCAAGAAGCACCTGATAGTTTCAGGAATGCTGGGAGACACAATT TTAACACACTTTGTTTCCAGTTTCACCTGTGGTTTGGCTGGGGCTCTGGCATCTAACCCTGTGGATGTGG TGAGAACTCGAATGATGAATCAGAGGGCAATAGTGGGACATGTGGACCTCTACAAGGGTACTTTGGATGG TATTTTAAAGATGTGGAAGCATGAGGGATTTTTTGCACTCTATAAAGGATTTTGGCCAAACTGGCTTCGA CTTGGACCCTGGAACATCATTTTTTTTATTACCTATGAGCAGCTCAAGAGGCTTCAGATCTAAGACCTGG AAACCCATCAGCCCAGCCCTGCCACCGTTTCTACCGGTCCTCTTTGTGTTCCTGATGTATTTTGACAAAG ATGTTTGTGTTTACAGAAGCAGCGGGCTGAAGAGTTGTAGGGTGACTGAAGGTTGTTTTGCATTTGTGTT GCCTCTTCCCTGAGAGGAAGTATCGACTTTGAGAGTGTGACCCCAGATTGGTGTCTTCTGTGAAGATGGA TACTGGTGGGTGACATTCAAAAGGGCCTTCTTTTCAACTGTGAGTTAAACGTAGTTGGTTTTCCCCAGAC AGGCTACAGCAGCAGGCTACCAGCACAGTTCCTGCTATGTATGCTTTTGACCTTGGTTCTCATTAAAGTA TTTATTGGTTGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6333237 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6333237 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004145 same experiment
	EMAGE:30515 same embryo
	EMAGE:30494 same embryo
	EMAGE:32116 same embryo
	EMAGE:30721 same embryo
	EMAGE:30511 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source