EMAGE mouse gene expression spatial database

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EMAGE:31649

Olfr288 ( MGI:3030122)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005242_01	euxassay_005242_02	euxassay_005242_03	euxassay_005242_04	euxassay_005242_05

euxassay_005242_06	euxassay_005242_07	euxassay_005242_08	euxassay_005242_09	euxassay_005242_10

euxassay_005242_11	euxassay_005242_12	euxassay_005242_13	euxassay_005242_14	euxassay_005242_15

euxassay_005242_16	euxassay_005242_17	euxassay_005242_18	euxassay_005242_19	euxassay_005242_20

euxassay_005242_21	euxassay_005242_22	euxassay_005242_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

oral epithelium

strong

spotted

strong expression: see section 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09

mandible

moderate

regional

moderate expression: see section 08 09 10 11 12 15 16 17 18 19 20

lung

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 14 15 17 18 21 22 moderate expression: see section 13 16 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T63
Entity Detected:	Olfr288, ( MGI:3030122)
Sequence:	sense strand is shown >T63 CTGGGGAAACTTCTCCAGACACCCTCCTTCTGGGCAGATCCTGCCTAAGCCAGGCTGCCTCTCCCAAGAG CCCGCCCCATGGCCCCCTTCATTTCCTTGTTGCGGAAGCTGTAGATGACCGGGTTGCACATGGGCATGAT GATGGTATAGAGGAGGGAGAAAGACTTGTCTTTGTCAGGCCCATGTGTACTGCGGGGATTCATGTAAGAA AACATGGCTGAAGTGTACAAAAAGATGACCACAGTCAGGTGGGAGGCACAAGTAGAAAAGGTCTTCCTAT GGCTTGAGGAGGAGGCCCTGTGGAGGATGGAGGCCAGGATGCGGGCATAGGAGGTGATGATGAGCACCAT GGGACTGAGCAACACCACAATGGCATCCACAAAGATCAACTTCATGGTGAACTTGAGGTCCCCGCAGGAG AGAGCGATCACTATGGGAGCCTCACAGAAGAAGTTCTCTATGTGGTTGTCTTTGCAGAAGGGATTCCTGA ATGATATATACTCAAGAAAGATACCATTGACC
Notes:	The probe template was PCR amplified from IMAGE:1123486 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1123486 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005242 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source