EMAGE mouse gene expression spatial database

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EMAGE:31655

Phyh ( MGI:891978)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004803_01	euxassay_004803_02	euxassay_004803_03	euxassay_004803_04	euxassay_004803_05

euxassay_004803_06	euxassay_004803_07	euxassay_004803_08	euxassay_004803_09	euxassay_004803_10

euxassay_004803_11	euxassay_004803_12	euxassay_004803_13	euxassay_004803_14	euxassay_004803_15

euxassay_004803_16	euxassay_004803_17	euxassay_004803_18	euxassay_004803_19	euxassay_004803_20

euxassay_004803_21	euxassay_004803_22	euxassay_004803_23	euxassay_004803_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

heart ventricle

weak

homogeneous

weak expression: see section 06 07 08 09 10 11 12 13 14 15

liver right lobe

moderate

regional

moderate expression: see section 14 15 16 17 18 19 20 21 22 23 24

right lung

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21

stomach

weak

regional

weak expression: see section 02 03 04 05 06 07

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 05 16

left lung

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

midgut loop

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12

dorsal root ganglion

moderate

regional

moderate expression: see section 05 12 13 14 weak expression: see section 03 04

adenohypophysis

weak

regional

weak expression: see section 09 10 13 14

submandibular gland primordium

strong

regional

strong expression: see section 06 07 08 09

vagus x ganglion

moderate

regional

moderate expression: see section 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6045
Entity Detected:	Phyh, ( MGI:891978)
Sequence:	sense strand is shown >T6045 CGTAAGTTAAGTGTGACCTGGGCGCAGCAGTTTCGTCTGCTGGCCCCCACCATGAATCTTACGCGCGCCG GCGCCCGGCTGCAGGTCCTTCTGGGACACCTCGGCCGACCCTCGGCCCCAACGATTGTAGCCCAGCCTGT ATCAGGGTTGGCTTCCCCTGCCAGTTTCCAGCCTGAACAATTCCAGTATACTCTGGATAATAATGTCCTC ACCCTGGAGCAGAGAAAATTTTATGAAGAAAATGGGTTTCTCGTTATTAAGAATCTGGTATCTGATGATG ACATTCAACGTTTTCGAGCAGAGTTTGAAAGAATCTGCAGAGAGGAGGTGAAACCACCAGGGATCGTTAT AATGAGAGATGTGGCCCTTGCAAAACAGGATTATATGCCAAGTGATAGGATGGTTTCAAAGATCCAGGAT TTCCAAGAAGATGAGGAGCTCTTCAGATACTGCCTTCTCCCCGAGATTCTGAAGTATGTGGAGTGTTTCA CTGGACCCAATATTATGGCTCTGCATGGGATGCTGATCAACAAGCCTCCAGATGTTGGCAAGAAGACATC CCGGCATCCCTTGCATCAGGATCTGCACTATTTCCCCTTCCGACCTAGCAACCTAATTGTTTGTGCTTGG ACAGCCATGGAGCACATTGACAGAAACAACGGTTGTCTGGTTGTGCTCCCAGGTACCCACAAAGGCACTC TGAAGCCACATGATTACCCCAAGTGGGAGGGAGGTGTTAACAAAATGTACCATGGCATCCAAGACTATGA CCCCAACAGCCCCCGGGTCCACCTAGTGATGGAAAAGGGCGATACTGTTTTCTTCCACCCTCTGCTCATC CATGGATCTGGTCGGAACAAAACTCAAGGCTTCCGGAAAGCAATTTCCTGCCACTTCGGCAGCTCTGACT GCCAGTGTATCGATGTGAGCGGCACCAGTCAAGAAAATATTGCAAGGGAAGTTGTCGAGATGGCAGAGAA AAAGTATGGATTCCAAGGAGTCATGGACTTTAAGGACACTTGGATATTTCGAAGCCGACTTGTGAAAGGA GAAAGAATAAACATTTGAAACAGCCCTTCAAGAGTGAGGTACTGGGATGATAGTCTGAGACAGGAGCATC GAGAGTTCGAGTACAGCCTGAGCTACACAGAAGGATGCTATCTCCTCCTCTTTCCCCCATAAACAAAATG AAAAGAGTTAGCTCTTCCTTATAATTCCTTTAAAGAGGACATCAAAGCAAAGAAAGACATCTAAGGAAAA AAATTTTTAACTGAGATGATGTTCTCTTTTTATCACCTGTTAACAAGATCAAGATGCACGTATTGGGGAT TCATGAATTTAATTAATGTGGTACTATTGTCTTAATGGAGTGAAATATTACTGTTTTAAGAAAAACTAAC CTAGGGTGTAACTAATAAAGGTGACTGAGTATAATGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3489939 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3489939 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004803 same experiment
	EMAGE:29946 same embryo
	EMAGE:31658 same embryo
	EMAGE:30250 same embryo
	EMAGE:30232 same embryo
	EMAGE:30213 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source