EMAGE mouse gene expression spatial database

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EMAGE:31658

Slc39a3 ( MGI:2147269)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004805_01	euxassay_004805_02	euxassay_004805_03	euxassay_004805_04	euxassay_004805_05

euxassay_004805_06	euxassay_004805_07	euxassay_004805_08	euxassay_004805_09	euxassay_004805_10

euxassay_004805_11	euxassay_004805_12	euxassay_004805_13	euxassay_004805_14	euxassay_004805_15

euxassay_004805_16	euxassay_004805_17	euxassay_004805_18	euxassay_004805_19	euxassay_004805_20

euxassay_004805_21	euxassay_004805_22	euxassay_004805_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

moderate

regional

moderate expression: see section 02 03 04 05 22 23

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 04 05 16 moderate expression: see section 06 14 15

cervico-thoracic ganglion

strong

regional

strong expression: see section 06 12

spinal cord

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11

brain

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 20 21 22 23 moderate expression: see section 01 02 03 04 18 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 04 05 14 15

trigeminal v nerve

strong

regional

strong expression: see section 09 10 15

thoracic ganglion

strong

regional

strong expression: see section 08 09 10

dorsal root ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 11 12 13

facial vii ganglion

strong

regional

strong expression: see section 03 04 05 06 18 19

not examined

regional

not examined expression: see section 02 03 04 05 22 23

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 15 16 17 18 19 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 12 14 16 17 weak expression: see section 10 11 15 18

cervical ganglion

strong

regional

strong expression: see section 06 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6047
Entity Detected:	Slc39a3, ( MGI:2147269)
Sequence:	sense strand is shown >T6047 GCCTGGCCCTGCGACTGGGGCCGGTAGCCCCCACAGCCTTTGGCCTGTGACTCCATTTGTGCTTTGGCCC TGTGTGACCCTGGCGGCCACCATGACGAAGCTTCTGGTGGCCAAGGTCCTCTGCATGGTGGGCGTGTTCT TCTTCATGCTGCTGGGCTCCCTGCTGCCTGTGAAGGTCATCGAGGCTGACTTGGAGAAGGCGCACCGCTC CAAGAAGGTCCTGTCCCTCTGCAACACCTTCGGGGGTGGCGTATTCCTGGCTACATGCTTTAATGCACTG CTGCCTGCAGTGAGGGACAAGCTGCAGCAGGTGCTGAGCCTGGGCCACATCAGCACCGACTACCCGCTGG CGGAGACGCTCATGATGGTGGGTTTCTTCCTCACTGTGTTCGTGGAGCAGCTGGTGCTGACCTTCCGCCG GGAGCGGCCTCCCTTTATAGACCTGGAGACCTTCAACGCCGGCTCAGATGCGGGCAGCGACTCGGAGTAC GAGAGCCCGTTCGTGGGTGTGGGCAACCGTAGCCACAGCCTGTACCCTGAGCCCACAGCGCACACCCATG GCGCGGGGCTGCGGCTGCGGGAACTGGGCCGCCCAGGGCCTCTGCGGCTGCTCAGCCTGGTCTTCGCGCT GTCTGCACACTCTGTGTTCGAGGGCCTGGCGCTGGGGCTGCAGGAGGAGGGTGAGCGTGTGGTCAGCCTG TTTGTTGGCGTGGCCATCCACGAGACGCTGGTGGCCGTGGCCCTGGGCATCAGCATGGCCCGCAGTGCGG TACCCCTGAGGGATGCGGCCAAGTTGGCTGTGACCGTGAGCGCCATGATCCCAGTGGGCATTGGGTTGGG CCTGGGCATCGAGAGTGCCCGCAGCGTGGCCAGCAGTGTGGCATCGGCGCTGCTACAGGGCCTGGCCGGT GGCACCTTCCTGTTTGTCACCTTCCTGGAGATCCTGGCCAAGGAGCTGGAGGAGAGGAGCGAGCAGCTGC TGAAGGTGCTGTTCCTGGTGCTGGGCTACGCCGTTCTGGCCGGAATGGTCTTTCTCAAGTGGTGAGCCCT GAATCCCTGAAGGACACAGAGCACGTCCCTTGACAACCACGCTCATGGGCCATGGTTCACACACAGAGGC CTCCCGGGCTGGTCACCGGTCTCTGCCATCCGCCAGAATCTGGCCCTACAGACACTGCTTTTAAAAACGT CTTAAAGTTCTCTACCAAAGCCTGTGTGGCCATGTCACCTCATATCCCGTCACCCCTCCAGTTTTTGTTG TCATTCCTGAGCCATCAAGAATGGAGCCCAGGGTGGGGACTGGCAGATGGGGATCAAAGTGACCTCAGGG ACCCTGCCATGTTCTCTGTGGAGCCCAGGCCCTCCCTGCCACTGCCACCCGAGTTTTTCTGGTTGCTCCT GTGTGACAGGTGACAGTTTATGAAAGGAACAGTACATGTGGGTCACATGAGCAAGGGGACAGCCCAGACT GTTTGCCTGGCCCTCAGGAACAGCCCTTCTTGTTCTCTTGCTCTGTGGTGTGGCGATCACCAGAGTGGAG GGGCCTCCTCTGCAGAGGGCATAGCAGTGAGACTCCAGGGCCACATCCTGACAGGGACAACTCCCAGGAT GCAGGCAGGAGCAGGGGAAACTCAGCAGCTGGCAACGTTGGTGCATGTCACCATGGTAATGCCAGCATCT TGGGACAGCCTGAGTGAGGACTCTCTGGGGACATAAGCCTGCCACAGCTCACCACCTTCCCTAACCTGGG GTCTGTGTAGCAGGCTTCACCTCAGCACTGCCTTCCCTCCGTTAGCTGAATGTCAGACTCCTCCCTAGTG CCCTGTCGCCATGGCCAGGTGACCAAAATGACTTGTCGAGTGTGCCCCTACCCAGCGACACTCAGATGAT ACAAGGCACAAAGAGACATGGCCTTGGGGACAGCTGGGGCTTGGGCATTGTCCTCAGAGCCCCAATGCCT GGGCTCCCTTAGTGCTGTAATACACCCAGGTTCACACGCCGCTTGGCACACATTCAGAACCCCGGGCTCC CAGTGAAGGGCCCACTCTGCCAGTGGTTGAAGCCTCTGCTAGCAGTTTCTGGGGTTCTTTCCCGAGGAGC CCCATCATGTAGATGTGGTGATGTACCCACAGTGCCACCTGGGCTTACTGTCCTTCTATCCTCTACTTAA CTATGGGTGGGTGGGGGGCATCCAAATGGTTCTCCAGCTTCCCTTGGCCCCAGGTGGGCCCCACGGGGCC ACCATGCCCCTGCAGGTATGGGCTCAGGTGACAAAGACTAGGCCAGGGTAGGAAGGTCCTTAGTCTGGCC ATCCCAACAGGGAGTCTGCACCTCCCACATGGGTGCATACAATGGCATTCTCTCCTTTCCAGCACCAACT GCTCTGCCCCTGGGGACAGCAGCAGCAGTGGATCTGGACCCTCCCCATTAGATCTCTTGAGTCTCTGAGA GTCATCCCAAGTGCCAGCTCACTGGCAACTGGGGGCCTCTGTGTCTCTTACTGAAAGTTCCAGTTTAGCT GACAGCCCTTGCCCCAGCTGCAGGAGACCCTGGGCCATGCTAGGACCTGTGCAGCTGTCAGGAGGAATGG GCATGGAGGAGAAATGGACATGCCGGGATGCTGCAGGGGCCAGGCCACCCCCACCCCACTGCCTCCTGTG GGGGACACAGCAGGAACACAGCTAGACAGGAGGAAAGAGGGCGGTGCGGGTGGCCCAGCTTCACTGCTGA TCTGTGTGTGTAATAACAGCATGTCAGCTTCTCCTATGGCTTGTTTGTGTTCCTGTAATAGAAGTTATTA GTGCAGGCGGGATCCGGCACAGCCAGCTCCTGGCATCCTGCCCTCATTGCACTGCAGGGGCCTGACCGAT CTTTGCCCTGCATCCCACCTGCCCAAGGAAGCTTTTCAGCTTGGTATTCCCCCACGGGGCGAGGGTGTTG TCTGTGGGGCACCCCGGGCTCCACGTGCACCTTCCCCCACTGTTGTCTCTGGAGTCCACCTGCTCTGAGC CAGGAGCTCCATGACATGTGTCCCAGATGTCATGCATAGTCCTCCTGGGGAGGGGTCCCACCTGTAAGTC AGCCCCCAATGATGAGAGGGATGACACCAGAACACCACAGCATCATTAGTGAACGCTCCCACAGAAGGGA AATCCAGGGACGCTGTCGGTGAGGAGCAAGCGTGGCAGACCTGAGTGCGATTCCAGGGGCTCACGTGCTG ACAGAACTGACTCCTGCACTGTCCCGATCTCCACGGGTGCTTAGTGGCACACATGCACACACTTGTGTGT GCTCGTCCCACACCCCCTGATATGTGTAATAACAGTTTTTAAGCTAGCCATGGTTACCCACTCCGTTAAT CCATGCACTCCAGATACAGGTGGATTTCTGTGAGTTCCAGGCCAGCCAGGGTTGCAAGTGGGCCCTTTTG CAAAATAAATAAACAAGTGAAGATTTAAACTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3490154 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3490154 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004805 same experiment
	EMAGE:29946 same embryo
	EMAGE:30250 same embryo
	EMAGE:30232 same embryo
	EMAGE:31655 same embryo
	EMAGE:30213 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source