EMAGE mouse gene expression spatial database

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EMAGE:31663

Slc7a4 solute carrier family 7 (cationic amino acid transporter, y+ system), member 4 ( MGI:2146512)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001779_01	euxassay_001779_02	euxassay_001779_03	euxassay_001779_04	euxassay_001779_05

euxassay_001779_06	euxassay_001779_07	euxassay_001779_08	euxassay_001779_09	euxassay_001779_10

euxassay_001779_11	euxassay_001779_12	euxassay_001779_13	euxassay_001779_14	euxassay_001779_15

euxassay_001779_16	euxassay_001779_17	euxassay_001779_18	euxassay_001779_19	euxassay_001779_20

euxassay_001779_21	euxassay_001779_22	euxassay_001779_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

midbrain

weak

homogeneous

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

telencephalon marginal layer

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

olfactory cortex ventricular layer

weak

regional

weak expression: see section 17

olfactory cortex marginal layer

weak

regional

weak expression: see section 10 11 12 13 15 16

hindbrain

weak

homogeneous

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

spinal cord

weak

homogeneous

weak expression: see section 09 10 11 12 13 14 15 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4367
Entity Detected:	Slc7a4, solute carrier family 7 (cationic amino acid transporter, y+ system), member 4 ( MGI:2146512)
Sequence:	sense strand is shown >T4367 CAGGTCCGCTGCAGGTCTGCTTCTCTCTGCCAGTTCCACTGCCCTGAAAGCTGACAGGAAGGACTTCCAT GCGTTCTATCCCTCTGCCCACAGTCCCTGACACGCGTCCCATTCAAGCCTTCTAAAGTTCCAGAAACTGG TTCAGTACTCCGTCTGCCATGGCCCGGGGACTGCCCAGTACTGCCTGTTTGGCACGCTTCTGCCAGAAAC TGAACCGTCTGAAGCCACTGGAAGAGTCTAGCATGGAGACATCACTGCGGCGCTGCCTGTCCACACTGGA CTTGACTCTACTGGGTGTGGGTGGCATGGTGGGATCTGGGCTCTATGTGCTCACAGGCACAGTGGCCAAG GACATGGCTGGCCCCGCTGTGCTCTTGTCCTTTTTGGTGGCCGCTGTAGCCTCCCTGCTGGCGGCCCTAT GCTATGCAGAGTTTGGAGCCCGTGTGCCCCGTACTGGCTCAGCATACCTATTCACGTACGTGTCCATGGG GGAGATATGGGCATTCCTCATAGGCTGGAATGTGCTTCTAGAATACCTCATTGGAGGTGCCGCTGTAGCC CGTGCCTGGAGTGGCTATTTGGATGCCATCTTTAACCACAGCATTCGCAACTTCACGGAGTCTCACCTGG GCGTCTGGCAGGTGCCCTTCCTAGCTCACTATCCAGATTTTCTGGCCGCTGGCATTTTACTCGTGGCTTC TGCCTTTGTCTCCTGCGGAGCCCGAGTCTCCTCCTGGCTTAATCACACGTTCTCAGCCATCAGTCTGATT GTGATCCTCTTCATCATTGTCCTGGGTTTCATCCTGGCCCGTCCTCACAACTGGAGTGCAGAAGAAGGTG GTTTCGCACCCTTCGGCTTCTCTGGCATCCTGGCTGGCACAGCCACCTGTTTCTATGCCTTCGTGGGATT TGATGTTATTGCTGCCTCCAGTGAGGAGGCCAAAAACCCACGGTGGGCGGTGCCCATGGCCATCGCCATC TCCCTCAGCCTGGCAGCTGGTGCCTATATTCTGGTCTCCACTGTGTTAACCCTCATGGTACCTTGGCACA GCCTAGACCCTGACTCGGCGCTTGCTGATGCTTTCTACAGGCGGGGTTACAGCTGGGCTGGCTTCATTGT GGCAGTTGGCTCTATCTGTGCCATGAACACCGTCCTGCTCAGCAACCTCTTCTCCCTGCCGCGCATTGTC TACGCCATGGCTGCCGATGGGCTCTTCTTCCAGGTGTTTGCCCGTGTACACCCCCGGACACAGCCTCCTG GTCCTGGGGGCTCATCAGCAGCAAAAGAAGCAAGACACTTTCCAGATCCCTTTGGTGCCCCTGACTCCAG CCCTGAGCATCCTTCTCAACACTTGCCTCATGCTGAAGCTGAGCTACCTGACCTGGCTACGCTTTATCTT CTGGCTGCTGGTCGGACTTGTCGTGTATTTTGGCTATGGCATCTGGCACAGCAAGGAGAACCAGAGGGAG CCATTGGAGCTGACTACAGCACACTACGTGGTATTCCCCAGCGGTAGCCTGGAGGAAACTGTGCAAGCTG TGCAGCCCTCTAGTCAATCACCAGTCCGGGAGTCAGGTTGTACTGAGTAGCTGGCCACGGAGGCTTGCTC ATGGAGGTCTCCATCCCCAACATCCAGAGGCCAGAAGAATTGGCAGCTGCCTACTTAAGGAAGCTTGGGT TTGAAGCCTGCCCAGACTGGGCTGGGAGTGTTCTCAGAGTCTCAACACAGGAACGGAGTCTTCGGCTATC ATGGACTAGGCTGAGGCTAGTGCTGGTGTGGACTCGTTGCCCACTGTCTTCCTGTTTATGATTAACTCTG GATACCTGGGCAGGTGGGGCAGAGTGGGCAGGGAAGTGGCTTCAGCCCCTGAGACCCAGAATAATAACTA ATCAGCTAGTGTTGTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4911158 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4911158 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001779 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source