EMAGE mouse gene expression spatial database

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EMAGE:31668

6030423D04Rik ( MGI:2441706)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_009254_01	euxassay_009254_02	euxassay_009254_03	euxassay_009254_04	euxassay_009254_05

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euxassay_009254_11	euxassay_009254_12	euxassay_009254_13	euxassay_009254_14	euxassay_009254_15

euxassay_009254_16	euxassay_009254_17	euxassay_009254_18	euxassay_009254_19	euxassay_009254_20

euxassay_009254_21	euxassay_009254_22	euxassay_009254_23	euxassay_009254_24	euxassay_009254_25

euxassay_009254_26

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 18 19 20 21 22 23 24 25 26 moderate expression: see section 06 07 08 09 10 11 17

foot

strong

regional

strong expression: see section 22 23

leg muscle

strong

regional

strong expression: see section 01 02 03 04 21 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26

thymus primordium

strong

regional

strong expression: see section 11 12 13 14 15

arm muscle

strong

regional

strong expression: see section 01 02 03 04 05 21 22 23 24 25 26

lower leg rest of mesenchyme

strong

regional

strong expression: see section 26

extrinsic ocular muscle

strong

regional

strong expression: see section 18 19 20 21 22 23

tongue

strong

regional

strong expression: see section 11 12 13 14 15 16 17

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 04 26

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T587
Entity Detected:	6030423D04Rik, ( MGI:2441706)
Sequence:	sense strand is shown >T587 TCCTCGAGNCTGTTGGCCTACTGGACTTGCCGCCAGCCAGGGAAGGTGCTGAGGACCCAACACAAGGAAC GGCTTCAGGGAGCTCGGCAGCTACAGTTCCTCAAAAGGAGAAACCTGGAAGAAGAGAAGAAAGGCCAAGC CAGGGAACAAGGGCCCTCCAGCAAGACAGATGGAGGAACAGGCCAAGTGTCTATCCTCAAGGAGTCCTTG CCTGGTGCCAACAAGGCCTCTTTCCCTGGACAGCAGGAAACAGGGATAAGCTCTGAGGTTTTCCCGGCCT TACATCATTCCTCCTCAGGCATCCAGAGGGATCTGGGTGGCCACCATGCCTCCCATGGGAGAGCCTTTCC ACCACAGGACTCTGACATCAAGAAGCCACACAGACAGCACCGGGGCACTCAGACAAAGGCAGAAGAGGCA TTGCCAACAATAAAGAATGATGCCAGTCAGCAAACCAAATGTGGAGTTGCTGTCCTAGACAAGGACATCA TTCAACTCTCGGAATACCTCAAAGAAGCCTTACATAGGGAGCTGATCCTAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1885445 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1885445 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_009254 same experiment
	EMAGE:31703 same embryo
	EMAGE:29651 same embryo
	EMAGE:29654 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source