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EMAGE:31690

Mapkapk3 mitogen-activated protein kinase-activated protein kinase 3 ( MGI:2143163)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31690 EMAGE:31690 EMAGE:31690 EMAGE:31690 EMAGE:31690
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
ventral grey horn
strong strong
regionalstrong expression: see section 13
stomach
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 moderate expression: see section 07 08 09 10 11
kidney calyx
moderate moderate
regionalmoderate expression: see section 07 weak expression: see section 08 18 19
rectum
moderate moderate
regionalmoderate expression: see section 13
urethra of male
moderate moderate
regionalmoderate expression: see section 12 13 14
midgut
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19
bladder
moderate moderate
regionalmoderate expression: see section 12 13 14
esophagus
moderate moderate
regionalmoderate expression: see section 12 13
thymus primordium
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 not examined expression: see section 01 02 03 04 05 06
adrenal gland
moderate moderate
regionalmoderate expression: see section 08 09 10 16 17
vibrissa
strong strong
regionalstrong expression: see section 06 07 22 23 moderate expression: see section 08 21 weak expression: see section 04 05 24
hindgut
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16 17
epidermis
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
naris
moderate moderate
regionalmoderate expression: see section 12 13 14 17 18 19
liver lobe
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 12 13 17 18
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 12 13 17
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1056
Entity Detected:Mapkapk3, mitogen-activated protein kinase-activated protein kinase 3 ( MGI:2143163)
Sequence:sense strand is shown

>T1056
TCGAGNCTGNNTGGCCTACTGGGAATATGCACCACGGCAAGCGCTGTCTCCTCATTGTCATGGAATGCAT
GGAGGGTGGTGAGCTGTTCAGCAGGATTCAGGAGCGTGGTGACCAGGCTTTCACTGAGAGAGAGGCTGCA
GAGATAATGCGGGACATTGGCACTGCCATCCAGTTCTTGCACAGCCGGAACATTGCCCACCGAGATGTCA
AGCCTGAAAACCTACTCTATACATCCAAGGAGAAGGGTGCTGTACTTAAACTCACCGATTTTGGCTTTGC
CAAGGAAACCACCCAAAATGCCCTCCAGACACCCTGCTACACTCCCTATTATGTGGCTCCTGAGGTCCTG
GGTCCAGAGAAGTATGACAAGTCCTGTGATATGTGGTCCCCTGGCGTCATCATGTACATCCTTTTGTGTG
GATTCCCACCCTTCTACTCCAACACCGGCCAGGCCATCTCTCCAGGAATGAAAAGAAGGATTCGCTTGGG
CCAGTATAGCTTCCCTAACCCTGAATGGTTAGATGTCTCTGAGGATGCCAAGCAGCTAATCCGCCTGCTC
CTGAAGACA
Notes:The probe template was PCR amplified from IMAGE:2088451 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2088451 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002875 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS