EMAGE mouse gene expression spatial database

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EMAGE:31693

Fam134b family with sequence similarity 134, member B ( MGI:1913520)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003968_01	euxassay_003968_02	euxassay_003968_03	euxassay_003968_04	euxassay_003968_05

euxassay_003968_06	euxassay_003968_07	euxassay_003968_08	euxassay_003968_09	euxassay_003968_10

euxassay_003968_11	euxassay_003968_12	euxassay_003968_13	euxassay_003968_14	euxassay_003968_15

euxassay_003968_16	euxassay_003968_17	euxassay_003968_18	euxassay_003968_19	euxassay_003968_20

euxassay_003968_21	euxassay_003968_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

weak

regional

weak expression: see section 06 07 08 13 14 15 16

facial vii ganglion

weak

regional

weak expression: see section 03 04 19

vagus x ganglion

weak

regional

weak expression: see section 07 16

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 17 18 19

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 weak expression: see section 18 19

heart ventricle

moderate

regional

moderate expression: see section 07 08 09 10 11 12 weak expression: see section 13

stomach

moderate

regional

moderate expression: see section 05 06 07 08 09

kidney calyx

weak

regional

weak expression: see section 05 06 07 14 15 16 17

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 05 06 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4126
Entity Detected:	Fam134b, family with sequence similarity 134, member B ( MGI:1913520)
Sequence:	sense strand is shown >T4126 GGAAAATGCTGGGCGACTGGGCAGCGCTCCTGCCCAGCTGCCTGTCACGGTGCTGGAGTGAGAGAGCCTG TGCTGTGGACAAGACAGCATTCTCTGGCCATCAGCACAAGCAGAGGCGGCTCAAGTTCTAATTCTCAAAG GGCAGAAATGCCTGCAGGCGGCGGCTGTGGACCAGGCAGAAGCTGGGAAGTCATCAATTCCAAGCCAGAT GAAAGAGCCAGACTGAGCCAGTGCATTGCAGAATCATGGATGAATTTCAGCATGTTTCTTCAAGAAATGT CTCTTTTTAAACAGCAGAGTCCTGGCAAGTTTTGCCTCCTGGTCTGCAGTGTGTGCACATTTTTTACAAT CTTGGGAAGTTACATTCCTGGGGTCATACTCAGCTACCTTCTGTTACTGTTTGCTTTTTTGTGTCCACTG TTTAAATGTAATGATATTGGACAAAAAATATACAGCAAAGTCAAGTCCATTCTATTAAAACTAGATTTTG GAATTGGAGAATATATTAACCAGAAGAAACGTGAGAGATCTGAAGCAGATAAAGAAAAAAGTCACAAGGA TGACAGTGAATTAGACTTTTCAGCTCTTTGTCCTAAGATTAGCCTCACAGTTGCTGCCAAAGAGTTATCT GTGTCAGACACAGATGTGTCAGAAGTCTCTTGGACTGACAATGGGACCTTCAACCTTTCAGAGGGGTACA CTCCGCAGACAGACACTTCTGACGATCTTGACCGGCCCAGTGAGGAAGTTTTCTCTCGAGATCTTTCAGA TTTTCCATCTCTGGAAAATGGGACGGGAACAAATGATGAAGATGAATTAAGCCTGGGCTTACCCACAGAG CTCAAGAGAAAGAAGCAGCAGTTGGATAGTGCTCACAGACCAAGCAAAGAACGGCAGTCGGCAGCTGGCC TCTCACTTCCTCTCAAGAGCGACCAAGCCCTTCACTTGATGAGCAACCTGGCTGGGGATGTCATCACAGC CGCCATGACGGCTGCCATCAAAGACCAGCTAGAAGGAGCCCGGCAGGCACTCACTCAGGTGGCGCCCACT GCGGGAGAGGACACAGACACTGAGGAGGGGGATGACTTTGAACTACTTGACCAGGCAGAGCTAGATCAAA TTGAGAGTGAGCTGGGACTAACACAAGACCAAGGGGCAGAAGCCCAGCAAAGTAAGAAATCCTCAGGCTT CCTCTCCAATCTGCTTGGAGGCCATTAACCGGGAATCACCTGGCAGCCAGCGAAGCACAGATAAACCACC CAAAAAGTTCAAACAAGCAGGGGGAAAAAATGGAAAAAAAATGGAATTGTAAGCTTTAATTACTTTAGTT TTTTTTTCTTTTGTTTTGTTTTCTTTTCCCTTCTATAGTGAGTTGGATGTGTATCAGTTGATAATGATAG ACTGACATTTCTGATAGTTATTTTTCTGTAATAAGCATGGAAATGAACTTTATAGTTACATATATACATA CTGTGGTGTCCTAAGTGCTTAGGGGCTGTTTGTAAAGTGAAGAGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4460992 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4460992 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003968 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source