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EMAGE:31720

Srp14 ( MGI:107169)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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euxassay_001753_01 euxassay_001753_02 euxassay_001753_03 euxassay_001753_04 euxassay_001753_05
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euxassay_001753_06 euxassay_001753_07 euxassay_001753_08 euxassay_001753_09 euxassay_001753_10
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euxassay_001753_11 euxassay_001753_12 euxassay_001753_13 euxassay_001753_14 euxassay_001753_15
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euxassay_001753_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 13 14 15 16
thymus primordium
weak weak
regionalweak expression: see section 08 09 10 11 12
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 16 17 18 19 20
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 05
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 05 16
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1402
Entity Detected:Srp14, ( MGI:107169)
Sequence:sense strand is shown

>T1402
TTCCTCGAGCCTGTTGGCCTACTGGAGTTTTCCGGCTGTAACCGCTGGAGGCTTCTGCTGACGGCGTCGG
ACCTGCGGAGCCCAGGATGGTGTTGCTCGAGAGCGAGCAGTTCCTGACGGAGCTGACCAGGCTCTTCCAG
AAGTGCCGCTCGTCGGGCAGCGTGTTCATCACCCTCAAGAAATATGACGGTCGCACCAAACCTATCCCGA
GGAAGAGTTCTGTGGAGGGCCTCGAGCCCGCAGAAAACAAGTGTCTGTTGAGAGCCACGGATGGGAAAAG
GAAGATCAGCACCGTGGTGAGCTCCAAAGAAGTGAACAAGTTTCAGATGGCCTATTCAAATCTACTGAGA
GCCAACATGGACGGGCTGAAGAAGAGGGACAAGAAGAACAAGAGTAAGAAGAGCAAACCAGCACAGTGAC
AGGCGTGGCTGCTACCAACCAGCTGCACAAGTGCATTTTTCCTCTGTTTGCTGCTTTCAGCACCTCTGTA
TGTAACTGTTTCCACGGAAGGGTCCTTTAAGAGAGAAGGACTGGGATGGGCTTGGGCTAGTTGTGGTAAG
ACGCCAGTTTTTGATTTG
Notes:The probe template was PCR amplified from IMAGE:2372828 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2372828 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_001753 same experiment
 EMAGE:31774 same embryo
 EMAGE:29574 same embryo
 EMAGE:29568 same embryo
 EMAGE:29721 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS